Effects of Short Chain Fatty Acids on Expression of Inflammatory Factors and G-Protein Coupled Receptor 41 in Bovine Rumen Epithelial Cells

YAN Kang, FENG Baobao, ZHAN Kang, JIANG Maocheng, GONG Xiaoxiao, ZHAO Guoqi, YU Lianqin, LIU Li

doi:10.3969/j.issn.1006-267x.2019.03.031

2019 , Vol. 31 >Issue 3: 1259 - 1265

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.03.031

Molecular Nutrition

Effects of Short Chain Fatty Acids on Expression of Inflammatory Factors and G-Protein Coupled Receptor 41 in Bovine Rumen Epithelial Cells

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1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Station of Animal Husbandry and Veterinary Medicine in Jiangsu Province, Nanjing 210017, China;
3. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China;
4. Animal Disease Prevention and Control Center of Jiangsu Province, Yixing 214206, China;
5. Langfang Polytechnic Institute, Langfang 065001, China

Received date: 2018-08-05

Online published: 2019-03-18

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Abstract

The experiment was aimed to investigate the effects of different concentrations of short chain fatty acids (SCFA) on the expression of inflammatory factors and G-protein coupled receptor 41 (GPR41) in bovine rumen epithelial cells. The experiment was divided into four groups. Every group contained three repetitions. The bovine rumen epithelial cells were incubated with 5, 10, 20 and 40 mmol/L SCFA, respectively. After culture of 24 h, cells were collected to extract the total RNA. The inflammatory factors, chemokine, and GPR41 experiment were analyzed by qRT-PCR. The results showed that the addition of 20 and 40 mmol/L SCFA significantly enhanced the expression of GPR41 compared with the addition of 5 mmol/L SCFA (P<0.05). Compared with 5 and 10 mmol/L SCFA, the addition of 20 and 40 mmol/L SCFA significantly enhanced the expression of interleukin-1β (IL-1β) (P<0.05). In comparison with the addition of 5 mmol/L SCFA, the addition of 10, 20 and 40 mmol/L SCFA significantly increased the tumor necrosis factor-α (TNF-α) expression (P<0.05). In addition, the addition of 5, 10 and 20 mmol/L SCFA did not alter the chemokine 20 (CCL20) expression (P>0.05), but CCL20 expression was significantly elevated by the addition of 40 mmol/L SCFA (P<0.05). Compared with 5 and the addition of 10 mmol/L SCFA, the addition of 20 and 40 mmol/L SCFA significantly enhanced the expression of chemokine 2 (CXCL2) and chemokine 8 (CXCL8) (P<0.05). With the increase of the concentration of SCFA, chemokine 3 (CXCL3) expression was significantly upregulated (P<0.05). In conclusion, SCFA can enhance the expression of GPR41, accordingly mediate the expression of inflammatory factors and chemokines, which mediates rumen epithelial protective immune response.

Key words： short chain fatty acids; inflammatory factor; chemokine; G-protein coupled receptor 41

Cite this article

YAN Kang, FENG Baobao, ZHAN Kang, JIANG Maocheng, GONG Xiaoxiao, ZHAO Guoqi, YU Lianqin, LIU Li . Effects of Short Chain Fatty Acids on Expression of Inflammatory Factors and G-Protein Coupled Receptor 41 in Bovine Rumen Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2019 , 31(3) : 1259 -1265 . DOI: 10.3969/j.issn.1006-267x.2019.03.031

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