Dynamic Effects of Antibiotic-Induced Intestinal Dysbacteriosis on Intestinal Mucosa Barrier and Liver Function

LI Na, ZHAO Yumin, CHEN Zhengyuan, WANG Bijun, LUO Xia, WANG Qing, HE Guolin, XIONG Tianqin, SANG Chuanlan

doi:10.3969/j.issn.1006-267x.2019.03.033

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 3: 1278 - 1287

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.03.033

Molecular Nutrition

Dynamic Effects of Antibiotic-Induced Intestinal Dysbacteriosis on Intestinal Mucosa Barrier and Liver Function

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1. Guangzhou University of Chinese Medicine, Guangzhou 510006, China;
2. Dengzhou Vocational and Technical College, Dengzhou 474150, China

Received date: 2018-08-31

Online published: 2019-03-18

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Abstract

In order to observe the dynamic changes of intestinal mucosa barrier and liver function for different degrees of intestinal dysbacteriosis models, mouse models were made which had intestinal dysbacteriosis caused by ceftriaxone sodium. After administration of ceftriaxone sodium for 3, 6 and 10 days, the intestinal contents were sampled to measure 16S rDNA gene, the histopathological changes of the ileum tissues were observed by hematoxylin-eosin (HE) staining, the tight junction conditions of ileum tissues were observed by transmission electron microscope (TEM), the positive expression condition of zonula occluden-1 (ZO-1) of ileum tissues was observed by immunohistochemical (IHC) method, the contents of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in in intestinal homogenate were assessed by enzyme-linked immunosorbent assay (ELISA) method, the liver function indexes such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in liver homogenate and histopathological changes of liver tissue were also evaluated. The results showed as follows:compared with the normal group, the relative abundances of Chryseobacterium, Prevotella and Pseudomonas increased gradually, and the relative abundances of Bacillus, Lotus and Staphylococcus gradually decreased in model group after administration of ceftriaxone sodium for 3, 6 or 10 days. The positive expression of ZO-1 in ileum tissues was gradually decreased and the tight junction among the intestinal villus epithelial cells was gradually disappeared with the extension of ceftriaxone sodium treatment. Compared with the normal group, the contents of TNF-α and IL-1β in intestinal homogenate of model group had no changes at pre-dysbacteriosis (after administration of ceftriaxone sodium for 3 and 6 days), but increased significantly after the continued dysbacteriosis (after administration of ceftriaxone sodium for 10 days) (P<0.01). Meanwhile, with the ongoing imbalance of intestinal flora, it could affect the liver, which showed the activities of ALT and AST in liver homogenate in model group were significantly higher than those in normal group (P<0.01), and hepatocyte swelling and disordered structure of hepatic lobule were obviously found. It is concluded that long-term intestinal dysbacteriosis can destroy the intestinal microecological balance, which is reflected in the contents of intestinal proinflammatory cytokines are gradually increased, and the liver function is also affected.

Key words： ceftriaxone sodium; intestinal dysbacteriosis; intestinal mucosa barrier; tight junction; liver damage

Cite this article

LI Na, ZHAO Yumin, CHEN Zhengyuan, WANG Bijun, LUO Xia, WANG Qing, HE Guolin, XIONG Tianqin, SANG Chuanlan . Dynamic Effects of Antibiotic-Induced Intestinal Dysbacteriosis on Intestinal Mucosa Barrier and Liver Function[J]. Chinese Journal of Animal Nutrition, 2019 , 31(3) : 1278 -1287 . DOI: 10.3969/j.issn.1006-267x.2019.03.033

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