Molecular Nutrition

Effects of Dietary Pectic Oligosaccharide on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus

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  • Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-08-14

  Online published: 2019-03-18

Abstract

This experiment was conducted to determine the effects of dietary pectic oligosaccharide (POS) on the ileal barrier function of weaned pigs challenged by rotavirus (RV). Twenty-eight healthy crossbred (Duroc×Landrace×Yorkshire) piglets weaned at 21 days of age were used in a 2×2 factorial design. The trial factors included POS treatment (control diet or supplementing 200 mg/kg POS diet) and RV challenge (oral gavage with RV or sterilized medium). On day 15, RV challenge was carried out. The trial duration was 18 days. The results showed that RV infection increased the diarrhea rate and positive rate of rotavirus non-structural protein 4 (NSP4) in ileal mucosa, significantly decreased the villus height and villus height to crypt depth ratio of ileal mucosa (P<0.05), significantly reduced the expressions of zonula occludens-1 (ZO-1), occludin, mucin 1 and mucin 2 in ileal mucosa (P<0.05), significantly enhanced the malondialdehyde content in ileal mucosa and E. coli population in ileal digesta (P<0.05), and significantly decreased total antioxidant capacity in ileal mucosa and the population of Lactobacillus and total bacteria in ileal digesta of weaned pigs (P<0.05). Dietary POS reduced the diarrhea rate and positive rate of rotavirus NSP4 in ileal mucosa of the RV-infected weaned piglets, and significantly alleviated the effects of RV challenge on ileal mucosa morphology, microbial population in ileal digesta, antioxidant capacity and expressions of occludin, mucin 1 and mucin 2 in ileal mucosa of weaned piglets (P<0.05). These results suggest that dietary POS can prevent the RV-induced diarrhea, which is associated with the improvement of ileal barrier function of weaned pigs.

Cite this article

MAO Xiangbing, CHEN Hao, CHEN Daiwen, YU Bing, HE Jun, ZHENG Ping, YU Jie, LUO Junqiu, LUO Yuheng . Effects of Dietary Pectic Oligosaccharide on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus[J]. Chinese Journal of Animal Nutrition, 2019 , 31(3) : 1288 -1294 . DOI: 10.3969/j.issn.1006-267x.2019.03.034

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