Effects of Bacillus subtilis Copper on Growth and Development, Organ Indexes, Copper Deposition and Serum Biochemical and Antioxidant Indexes of Offspring Rats with Congenital Copper Deficiency

LIU Guodong, WANG Baowei, HUANG Yanping, GE Wenhua, ZHANG Ming, YUE Bin, LI Kailing, KONG Min

doi:10.3969/j.issn.1006-267x.2019.03.044

2019 , Vol. 31 >Issue 3: 1367 - 1375

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.03.044

Molecular Nutrition

Effects of Bacillus subtilis Copper on Growth and Development, Organ Indexes, Copper Deposition and Serum Biochemical and Antioxidant Indexes of Offspring Rats with Congenital Copper Deficiency

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1. Food Science and Engineering of Qingdao Agricultural University, Qingdao 266109, China;
2. National Waterfowl Industrial Technology System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2018-08-22

Online published: 2019-03-18

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Abstract

This experiment was conducted to study the effects of Bacillus subtilis copper on growth and development, organ indexes, copper deposition and serum biochemical and antioxidant indexes of offspring rats with congenital copper deficiency. A total of 108 clean grade pregnant SD rats were selected and randomly divided into 6 groups with 18 replicates per group and 1 rat per replicate. Groups Ⅰ to Ⅲ were copper sulfate groups, and the dietary total copper contents were 1.5, 3.0 and 6.0 mg/kg, respectively; groups Ⅳ to Ⅵ were Bacillus subtilis copper groups, and the dietary total copper contents were 1.5, 3.0 and 6.0 mg/kg, respectively. The experiment lasted for 35 days. The results showed as follows:1) the body weight of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05), the body weight and body length of group Ⅵ were significantly higher than those of group Ⅳ (P<0.05), and there were no significant differences in body weight, body length and survival rate among other groups (P>0.05). 2) There were no significant differences in heart index, liver index, spleen index, kidney index and lung index among all groups (P>0.05). 3) The copper deposition in brain of groups Ⅱ and Ⅲ was significantly higher than that of group Ⅰ (P<0.05), the copper deposition in liver of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05). The copper deposition in brain of group Ⅵ was significantly higher than that of group Ⅴ (P<0.05), the copper deposition in liver of group Ⅵ was significantly higher than that of group Ⅳ (P<0.05). The copper deposition in brain of group Ⅳ was significantly higher than that of group Ⅰ (P<0.05). 4) The serum ceruloplasmin (CP) and alkaline phosphatase (AKP) activities of group Ⅲ were significantly higher than those of groups Ⅰ and Ⅱ (P<0.05 or P<0.01), the serum CP activity of groups Ⅴ and Ⅵ was significantly higher than that of group Ⅳ (P<0.05), and the serum CP activity of groups Ⅳ and Ⅴ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05), respectively. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities of group Ⅲ were significantly higher than those of group Ⅰ (P<0.01), the serum ALT activity of group Ⅵ was significantly higher than that of group Ⅳ (P<0.05), and the serum ALT activity of group Ⅳ was significantly higher than that of group Ⅰ (P<0.05). 5) The serum glutathione peroxidase (GSH-Px) activity of group Ⅲ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05), the serum copper-zinc superoxide dismutase (CuZn-SOD) activity of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05); the serum CuZn-SOD activity of group Ⅵ was significantly higher than that of group Ⅳ (P<0.05). In conclusion, the appropriate amount of Bacillus Subtilis copper has a rapid repair effect on the growth and development and antioxidant capacity of offspring rats with congenital copper deficiency, and its effect is better than copper sulfate.

Key words： Bacillus subtilis copper; copper sulfate; pregnancy and lactation rats; growth and development; antioxidant capacity

Cite this article

LIU Guodong, WANG Baowei, HUANG Yanping, GE Wenhua, ZHANG Ming, YUE Bin, LI Kailing, KONG Min . Effects of Bacillus subtilis Copper on Growth and Development, Organ Indexes, Copper Deposition and Serum Biochemical and Antioxidant Indexes of Offspring Rats with Congenital Copper Deficiency[J]. Chinese Journal of Animal Nutrition, 2019 , 31(3) : 1367 -1375 . DOI: 10.3969/j.issn.1006-267x.2019.03.044

References

[1] GAETKE L M,CHOW C K.Copper toxicity,oxidative stress,and antioxidant nutrients[J].Toxicology,2003,189(1/2):147-163.

[2] KIM B E,NEVITT T,THIELE D J.Mechanisms for copper acquisition,distribution and regulation[J].Nature Chemical Biology,2008,4(3):176-185.

[3] JU C F,YU B,ZHU D,et al.The effect of dietary sulphate copper supplementation on growth performance,intestinal digestive enzymes and absorptive transporters in weanling pigs[J].Journal of Animal Science and Biotechnology,2010,1(1):35-43.

[4] KUMAR V,KALITA J,BORA H K,et al.Temporal kinetics of organ damage in copper toxicity:a histopathological correlation in rat model[J].Regulatory Toxicology and Pharmacology,2016,81:372-380.

[5] SOETAN K O,OLAIYA C O,OYEWOLE O E.The importance of mineral elements for humans,domestic animals and plants:a review[J].African Journal of Food Science,2010,4(5):200-222.

[6] LEI X G,KU P K,MILLER E R,et al.Supplemental microbial phytase improves bioavailability of dietary zinc to weanling pigs[J].The Journal of Nutrition,1993,123(6):1117-1123.

[7] ARTHINGTON J D,PATE F M,SPEARS J W.Effect of copper source and level on performance and copper status of cattle consuming molasses-based supplements[J].Journal of Animal Science,2003,81(6):1357-1362.

[8] HUANG Y,YOO J S,KIM H J,et al.The effects of different copper (inorganic and organic) and energy (tallow and glycerol) sources on growth performance,nutrient digestibility,and fecal excretion profiles in growing pigs[J].Asian-Australasian Journal of Animal Sciences,2010,23(5):573-579.

[9] 刘冬,黄守敏,毕璋友,等.益生菌混合培养富集锌条件优化研究[J].安徽农业科学,2015,43(20):259-262.

[10] REEVES P G,NIELSEN F H,FAHEY G C,Jr.AIN-93 purified diets for laboratory rodents:final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J].The Journal of Nutrition,1993,123(11):1939-1951.

[11] WANG J G,ZHU X Y,GUO Y Z,et al.Influence of dietary copper on serum growth-related hormone levels and growth performance of weanling pigs[J].Biological Trace Element Research,2016,172(1):134-139.

[12] SIERPINSKA T,KONSTANTYNOWICZ J,ORYWAL K,et al.Copper deficit as a potential pathogenic factor of reduced bone mineral density and severe tooth wear[J].Osteoporosis International,2014,25(2):447-454.

[13] SMITH M S.Responses of chicks to dietary supplements of copper sulphate[J].British Poultry Science,1969,10(2):97-108.

[14] 潘娜,朱风华,王友令,等.日粮添加高水平硫酸锌和纳米氧化铜对鸡脏器组织锌铜沉积的影响[J].中国农业科学,2011,44(23):4874-4881.

[15] O'DONOHUE J,REID M A,VARGHESE A,et al.Micronodular cirrhosis and acute liver failure due to chronic copper self-intoxication[J].European Journal of Gastroenterology & Hepatology,1993,5(7):561-562.

[16] ARAYA M,OLIVARES M,PIZARRO F,et al.Copper exposure and potential biomarkers of copper metabolism[J].Biometals,2003,16(1):199-204.

[17] CHOLEWI ?SKA E,JU ? KIEWICZ J,OGNIK K.Comparison of the effect of dietary copper nanoparticles and one copper (Ⅱ) salt on the metabolic and immune status in a rat model[J].Journal of Trace Elements in Medicine and Biology,2018,48:111-117.

[18] MEGAHED M A,HASSANIN K M A,YOUSSEF I M I,et al.Alterations in plasma lipids,glutathione and homocysteine in relation to dietary copper in rats[J].Journal of Investigational Biochemistry,2014,3(1):21-25.

[19] OZKUL H,KIRKPINAR F,MERT S,et al.Effects of high levels of dietary copper sulfate and copper proteinate on growth performance,retention for copper and zinc of rats[J].Journal of Animal and Veterinary Advances,2011,10(11):1373-1377.

[20] ENGLE T E,SPEARS J W.Effects of dietary copper concentration and source on performance and copper status of growing and finishing steers[J].Journal of Animal Science,2000,78(9):2446-2451.

[21] 韩丽,孔祥峰,赵越,等.枯草芽孢杆菌对围产期母猪繁殖性能和子代生长的影响[J].动物营养学报,2017,29(12):4440-4446.

[22] 霍仕霞,凯赛尔·阿不都克热木,彭晓明,等.驱白巴布期对缺铜、缺锌大鼠血清中微量元素及免疫球蛋白水平的影响[J].中国现代应用药学,2011,28(8):689-694.

[23] 赵春雨,张天芮,刘博,等.日粮铜来源及水平对猪血液生化指标及含铜酶活性的影响[J].中国畜牧杂志,2015,51(21):39-44.

[24] 钱剑,刘国文,王哲,等.铜对软骨细胞碱性磷酸酶活性影响的实验观察[J].中国兽医杂志,2005,41(3):21-22.

[25] 林立,曾晓立,张璟.丙溴磷对家兔肝组织脂质过氧化及肝功能的影响(英文)[J].中国临床康复,2004,8(21):4380-4381.

[26] 张娇娇,朱风华,陈甫,等.纳米氧化铜对鸡肝细胞CP mRNA表达及培养基质中肝酶活性的影响[J].中国兽医学报,2012,32(9):1357-1361.

[27] 张寿,莫重辉,常兰,等.狼毒草对小白鼠组织中的超氧化物歧化酶、谷胱甘肽过氧化物酶、丙二醛的影响[J].黑龙江畜牧兽医,2014(9):105-107.

[28] HARRIS E D.Menkes' disease:perspective and update on a fatal copper disorder[J].Nutrition Reviews,1993,51(8):235-238.

[29] REZAIE A,PARKER R D,ABDOLLAHI M.Oxidative stress and pathogenesis of inflammatory bowel disease:an epiphenomenon or the cause?[J].Digestive Diseases and Sciences,2007,52(9):2015-2021.

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