Effects of Curcumin and Inhibitors of Mitogen Activated Protein Kinase Pathways on Jejunal Mucosal Morphology, Permeability and mRNA Relative Expression Levels of Tight Junction Proteins and Inflammatory Cytokines in Weaned Piglets

XUN Wenjuan, CAO Ting, SHI Liguang, HOU Guanyu

doi:10.3969/j.issn.1006-267x.2019.03.049

2019 , Vol. 31 >Issue 3: 1415 - 1421

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.03.049

Molecular Nutrition

Effects of Curcumin and Inhibitors of Mitogen Activated Protein Kinase Pathways on Jejunal Mucosal Morphology, Permeability and mRNA Relative Expression Levels of Tight Junction Proteins and Inflammatory Cytokines in Weaned Piglets

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Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Received date: 2018-08-31

Online published: 2019-03-18

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Abstract

The aim of this study was to investigate the effects of curcumin and inhibitors of mitogen activated protein kinase (MAPK) pathways on jejunal mucosal morphology, permeability and mRNA relative expression levels of tight junction proteins and inflammatory cytokines of weaned piglets. Thirty Duroc×Landrace×Large White healthy piglets with similar parity and body weight were weaned at 21 days of age and randomly allocated into 5 groups with 6 replicates in each group. Piglets in experimental groups received intraperitoneal injection of 2 mg/kg of curcumin (B group), inhibitors of p38 MAPK (C group), Jun N-terminal kinase (JNK, D group) and extracellular signal-regulated kinase1/2 (ERK1/2, E group) at the first day of the experiment, respectively. The same dose was injected every other day, and the same volume of saline was injected into the piglets of control group (A group). The piglets were inoculated with Escherichia coli at the second day of experiment. The experiment lasted for 5 days. The results showed as follows:1) compared with control group and E group, jejunum villus height and villus height/crypt depth in B and C groups significantly increased (P<0.05), and crypt depth significantly decreased (P<0.05); villus height/crypt depth in D group significantly increased (P<0.05). 2) The serum D-lactate and endotoxin contents in B, C and D groups were significantly lower than those in control group and E group (P<0.05), while serum D-lactate content in E group was significantly higher than that in control group (P<0.05). 3) Compared with control group and E group,the mRNA relative expression levels of occludin and zonula occluden-1 (ZO-1) in B, C and D groups significantly increased (P<0.05), the mRNA relative expression levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) significantly decreased (P<0.05), and that of interleukin-10 (IL-10) mRNA significantly increased (P<0.05). It is suggested that injection of curcumin, inhibitors of p38 MAPK and JNK pathways can improve the intestinal mucosal barrier function of piglets infected with E. coli, while there is no improvement in intestinal mucosal barrier damage by ERK1/2 inhibitor injection.

Key words： curcumin; MAPK signaling pathways; weaned piglets; jejumum mucosal morphology; tight junction protein; inflammatory cytokine

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XUN Wenjuan, CAO Ting, SHI Liguang, HOU Guanyu . Effects of Curcumin and Inhibitors of Mitogen Activated Protein Kinase Pathways on Jejunal Mucosal Morphology, Permeability and mRNA Relative Expression Levels of Tight Junction Proteins and Inflammatory Cytokines in Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2019 , 31(3) : 1415 -1421 . DOI: 10.3969/j.issn.1006-267x.2019.03.049

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