Effects of Orally Pichia anonmala AR2016 on Growth Performance and Safety of Kunming Mice

MA Yajun, LU Changwen, CHEN Qingju, TANG Zhiru

doi:10.3969/j.issn.1006-267x.2019.03.054

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 3: 1452 - 1462

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.03.054

Molecular Nutrition

Effects of Orally Pichia anonmala AR₂₀₁₆ on Growth Performance and Safety of Kunming Mice

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Key Laboratory for Bio-Feed and Animal Nutrition, Southwest University, Chongqing 400715, China

Received date: 2018-08-31

Online published: 2019-03-18

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Abstract

To comprehensively evaluate the bio-safety of Pichia anonmala AR₂₀₁₆ strain, this study was carried out to investigate effects of Pichia anonmala AR₂₀₁₆ strain on growth performance, serum biochemical indexes, hemogram indexes and immune function in Kunming mice. In experiment 1, a total of 20 Kunming mice were randomly assigned to one of two groups with 10 mice in each group (half male and half female). A 7-day oral toxicity test was performed at a dose of 0.2 mL per 10 g body weight; control group supplemented with normal saline, and experimental group supplemented with Pichia anonmala AR₂₀₁₆ strain at the concentration of 2×10⁹ CFU/mL. In experiment 2, a total of 40 Kunming mice were randomly assigned to one of four groups with 10 mice in each group (half male and half female). A 28-day oral toxicity test was performed at a dose of 0.2 mL per 10 g body weight; control group supplemented with normal saline, low dose group, middle dose group and high dose group supplemented with Pichia anonmala AR₂₀₁₆ strain at concentrations of 2×10⁷, 2×10⁸, 2×10⁹ CFU/mL, respectively. The results showed as follows:1) after 7 or 28 d treatment by Pichia anonmala AR₂₀₁₆ strain, no poisoning and death were found in mice, it was a "non-toxic" grade, and the maximum tolerated dose was more than 20 000 mg/kg. 2) Compared with the control group, after 7 d treatment by Pichia anonmala AR₂₀₁₆ strain, average daily feed intake (ADFI) of mice in experimental group significantly decreased (P<0.05); after 28 d treatment by Pichia anonmala AR₂₀₁₆ strain, ADFI of mice in each dosage group significantly increased (P<0.05). 3) Compared with the control group, after 7 d treatment by Pichia anonmala AR₂₀₁₆ strain, the contents of albumin and urea nitrogen in serum of mice in experimental group significantly decreased (P<0.05), and serum creatinine content and aspartate transaminase activity significantly increased (P<0.05); after 28 d treatment by Pichia anonmala AR₂₀₁₆ strain, serum biochemical indexes among groups had no significant difference (P>0.05). 4) After 7 d treatment by Pichia anonmala AR₂₀₁₆ strain, compared with the control group, white blood cell, hematokrit, lymphocytes count of mice in experimental group significantly increased (P<0.05), hemoglobin content of male mice in experimental group was significantly higher than that of male mice in control group (P<0.05), red blood cell count of male mice in experimental group was significantly higher than that of female mice in experimental group (P<0.05), neutral cell rate of female mice in experimental group was significantly lower than that of female mice in control group (P<0.05), and count of eosinophils of female mice in experimental group was significantly higher than that of female mice in control group (P<0.05); after 28 d treatment by Pichia anonmala AR₂₀₁₆ strain, blood routine indexes among groups had no significant difference (P>0.05). 5) Compared with the control group, after 7 d treatment by Pichia anonmala AR₂₀₁₆ strain, the content of interferon-γ (INF-γ) and tumor necrosis factor-α in serum of mice in experimental group significantly increased (P<0.05); after 28 d treatment by Pichia anonmala AR₂₀₁₆ strain, INF-γ content in serum of female mice in high dose group significantly increased (P<0.05). Therefore, the Pichia anomala AR₂₀₁₆ strain is non-toxic and high safety to Kunming mice, can increase ADFI, improve immune function, and having the value for further research and development.

Key words： Pichia anomala AR₂₀₁₆; Kunming mice; acute toxicity; safety

Cite this article

MA Yajun, LU Changwen, CHEN Qingju, TANG Zhiru . Effects of Orally Pichia anonmala AR₂₀₁₆ on Growth Performance and Safety of Kunming Mice[J]. Chinese Journal of Animal Nutrition, 2019 , 31(3) : 1452 -1462 . DOI: 10.3969/j.issn.1006-267x.2019.03.054

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