Effects of Dietary Different Non-Fiber Carbohydrate/Neutral Detergent Fiber on Nitrogen Metabolism, Rumen Environment and Volatile Fatty Acid Absorption of Fine Wool Sheep

YAN Cheng, JIN Lu, SUN Haizhou, SANG Dan, LI Shengli, ZHANG Chunhua, ZHANG Chongzhi, REN Xiaoping, SHAN Dan, LING Shuli

doi:10.3969/j.issn.1006-267x.2019.04.026

2019 , Vol. 31 >Issue 4: 1685 - 1694

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.026

Molecular Nutrition

Effects of Dietary Different Non-Fiber Carbohydrate/Neutral Detergent Fiber on Nitrogen Metabolism, Rumen Environment and Volatile Fatty Acid Absorption of Fine Wool Sheep

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Animal Nutrition and Feed Research Institution of Inner Mongolia Academy of Agriculture and Animal Husbandry Academy, Hohhot 010031, China

Received date: 2018-09-26

Online published: 2019-04-16

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Abstract

This experiment was conducted to investigate the effects of different non-fiber carbohydrate/neutral detergent fiber (NFC/NDF) on the nitrogen metabolism, rumen environment and volatile fatty acid (VFA) absorption. Nine Inner Mongolia Ordos of sheep with permanent rumen fistula were randomly divided into three groups on the basis of body weight. The NFC/NDF in diets of groups 1, 2, 3 were 0.35, 0.66 and 1.14, respectively, and the crude protein contents were all 13.50%. The results showed as follows:1) the NFC/NDF had no significant differences in nitrogen intake, fecal nitrogen and nitrogen deposition (P﹥0.05), but the urea nitrogen content in urine in group 1 was significantly higher than that in group 2 (P<0.05), and significantly higher than that in group 3 (P<0.01), and group 2 was significantly higher than group 3 (P<0.05). 2) After feeding 1.5 and 3.0 h, NH₃-N content in group 1 was significantly higher than that in group 3 (P<0.05), and after feeding 4.5 h, there was no significant difference among groups (P﹥0.05). The pH was 6.0 to 6.8, and pH in group 1 was significantly higher than that in group 3 (P<0.05). 3) After feeding 1.5 h, the acetic acid content in group 1 was significantly higher than that in group 2 (P<0.05), and significantly higher than that in group 3 (P<0.01). After feeding 1.5, 3.5 and 4.0 h, the propionic acid content in groups 1 and 2 was significantly lower than that in group 3 (P<0.05). Before feeding and after feeding, the butyric acid content in group 1 was significantly higher than that in group 3 (P<0.05). 4) The expression levels of Na⁺/H⁺ exchange protein 3 (NHE3)and down-regulated adenoma vector (DRA)genes in groups 2 and 3 were significantly higher than those in group 1 (P<0.05). The expression level of monocarboxylate transporter 4 (MCT4)gene in group 1 was significantly higher than that in groups 2 and 3 (P<0.05). The expression levels of Na⁺/H⁺ exchange protein 1 (NHE1) and Na⁺/K⁺ ATPase pump (Na⁺/K⁺ATPase) in group 2 were significantly higher than those in groups 1 and 3 (P<0.05). In conclusion, the rumen environment and the expressions of genes related with VFA absorption in fine wool sheep are significantly affected when dietary NFC/NDF is 0.66.

Key words： fine wool sheep; nitrogen metabolism; rumen environment; VFA absorption

Cite this article

YAN Cheng, JIN Lu, SUN Haizhou, SANG Dan, LI Shengli, ZHANG Chunhua, ZHANG Chongzhi, REN Xiaoping, SHAN Dan, LING Shuli . Effects of Dietary Different Non-Fiber Carbohydrate/Neutral Detergent Fiber on Nitrogen Metabolism, Rumen Environment and Volatile Fatty Acid Absorption of Fine Wool Sheep[J]. Chinese Journal of Animal Nutrition, 2019 , 31(4) : 1685 -1694 . DOI: 10.3969/j.issn.1006-267x.2019.04.026

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