Molecular Nutrition

Glycinin Induced Porcine Intestinal Epithelial Cell Damage Through p38 Mitogen Activated Protein Kinase/c-Jun N-Terminal Kinase Signaling Pathway

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  • College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2018-09-09

  Online published: 2019-04-16

Abstract

This experiment was aimed to study the effects of different concentrations of glycinin (11S) on porcine intestinal epithelial cells (IPEC-J2) cultured in vitro. The experiment was divided into six groups:groups A, B, C, D, E and F, group A was the control group, and the other groups were added 1, 5, 10, 5 and 5 mg/mL 11S, respectively, and 1 μmol/L JNK and p38 inhibitors were added to groups E and F, respectively. After 24 h culture, cell viability was measured by CCK-8 method; the contents of nitric oxide (NO), 5-hydroxytryptamine (5-HT), interleukin-6 (IL-6), and interleukin-10 (IL-10) were detected by enzyme linked immunosorbent assay (ELISA) method; phospho-c-Jun N-terminal kinase (p-JNK), phospho-p38 (p-p38) and B lymphocytoma-2 (Bcl-2) protein expression levels were detected by Western blot; the relative expression levels of Bcl-2/Bcl-xL related apoptotic protein (Bad), Bcl-2 related X protein (Bax), Bcl-2 and caspase-3 mRNA were determined by qRT-PCR. The results showed as follows:1) compared with group A, cell viability in groups C and D significantly decreased (P<0.01); compared with group C, cell viability in groups E and F significantly increased (P<0.05 or P<0.01). 2) Compared with group A, the contents of NO, 5-HT, IL-6 and IL-10 significantly increased (P<0.01); compared with group C, the contents of NO, 5-HT, IL-6 and IL-10 significantly decreased (P<0.05 or P<0.01). 3) Compared with group A, the expression levels of p-JNK and p-p38 protein in groups C and D significantly increased (P<0.05 or P<0.01), and the expression level of Bcl-2 protein in groups C and D significantly decreased (P<0.01); compared with group C, the expression levels of p-JNK and p-p38 protein in groups E and F significantly decreased (P<0.05 or P<0.01), and the expression level of Bcl-2 protein in groups E and F significantly increased (P<0.05 or P<0.01). 4) Compared with group A, Bcl-2/Bax in groups B, C and D significantly increased (P<0.01), and Bax/Bcl-xL and the relative expression levels of Bad and caspase-3 mRNA in groups C and D significantly increased (P<0.05 or P<0.01); compared with group C, Bcl-2/Bax in groups E and F significantly increased (P<0.01), Bax/Bcl-xL and the relative expression level of caspase-3 mRNA in groups E and F significantly decreased (P<0.01), and the relative expression level of Bad mRNA significantly decreased (P<0.01). The results indicate that 11S can induce IPEC-J2 damage through the p38 MAPK/JNK signaling pathway.

Cite this article

PENG Chenglu, ZHANG Yu, SHU Yingshuang, HE Mengchu, XIA Xiaodong, FENG Shibin, LI Yu, WANG Xichun, LI Jinchun, WU Jinjie . Glycinin Induced Porcine Intestinal Epithelial Cell Damage Through p38 Mitogen Activated Protein Kinase/c-Jun N-Terminal Kinase Signaling Pathway[J]. Chinese Journal of Animal Nutrition, 2019 , 31(4) : 1718 -1724 . DOI: 10.3969/j.issn.1006-267x.2019.04.029

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