Effects of Partially Replacing Fish Meal with Brewer's Yeast Meal on Growth, Antioxidant Capacity, and Liver and Intestine Tissue Morphology of Bullfrog[Rana (Lithobates) catesbeiana]

LI Xiao, WANG Ling, ZHANG Chunxiao, LU Kangle, SONG Kai

doi:10.3969/j.issn.1006-267x.2019.04.046

2019 , Vol. 31 >Issue 4: 1864 - 1876

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.046

Molecular Nutrition

Effects of Partially Replacing Fish Meal with Brewer's Yeast Meal on Growth, Antioxidant Capacity, and Liver and Intestine Tissue Morphology of Bullfrog[Rana (Lithobates) catesbeiana]

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Key Laboratory of Healthy Mariculture for the East China Sea of Ministry of Agriculture, Key Laboratory for Feed Quality Testing and Safety Evaluation of Fisheries College, Jimei University, Xiamen 361021, China

Received date: 2018-09-09

Online published: 2019-04-16

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Abstract

An 8-week feeding trial was carried out to evaluate the effects of partially replacing fish meal (FM) with brewer's yeast meal (BYM) on growth performance, body composition, liver antioxidant indexes, serum biochemical indexes, intestine digestive enzyme activities, liver and intestine tissue morphology of bullfrog[Rana (Lithobates) catesbeiana]. A total of 135 bullfrogs with an initial body weight of (34.15±1.10) g were randomly divided into 3 groups with 3 replicates per group and 15 bullfrogs per replicate. Three isonitrogenous and isolipidic diets were formulated. A control diet was formulated to contain 20% FM first, and then two other diets were prepared by substituting 25% (Y25%) or 50% (Y50%) of FM with BYM based on the control diet. The results showed that replacing 25% or 50% of FM with BYM did not significantly influence all growth performance indexes (P>0.05). The whole-body moisture content in two replacing groups was significantly higher than that in control group (P<0.05), and the whole-body crude protein and crude lipid contents had no significant differences compared with control group (P<0.05). The apparent digestibility coefficients of dry matter and crude protein in control group and Y25% group were significantly higher than those in Y50% group (P<0.05). With the replacing level of BYM increasing, the apparent digestibility coefficient of crude lipid was significantly increased (P<0.05), while the apparent digestibility coefficient of phosphorus was significantly decreased (P<0.05). Replacing 25% or 50% of FM with BYM had no significant effects on serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities (P>0.05). The activities of serum alkaline phosphatase (ALP) and lysozyme (LZM) were significantly higher than those in control group (P<0.05). The liver total antioxidant capacity in Y25% and Y50% groups was significantly higher than that in control group (P<0.05). The activities of liver total superoxide dismutase (T-SOD) and catalase (CAT) and the content of liver reduced glutathione (GSH) were significantly increased with the replacing level of BYM increasing (P<0.05). Histological observation showed that there were no obvious abnormal changes in liver and intestinal tissue structure of each group, but fat deposition was reduced in hepatocyte of Y25% and Y50% groups compared with control group. The relative expression level of heat shock protein 70 (HSP70) gene in liver in two replacing groups was significantly up-regulated compared with control group (P<0.05), while the relative expression level of insulin like growth factor-Ⅰ (IGF-Ⅰ) gene in liver was not significantly influenced by replacing FM with 25% or 50% BYM (P>0.05). According to the results, BYM can be used as a good protein source for bullfrog. Replacing FM with less than 50% BYM can keep regular growth performance and better liver health for bullfrog.

Key words： fish meal replacement; brewer's yeast meal; bullfrog[Rana (Lithobates) catesbeiana]; growth; antioxidant capacity

Cite this article

LI Xiao, WANG Ling, ZHANG Chunxiao, LU Kangle, SONG Kai . Effects of Partially Replacing Fish Meal with Brewer's Yeast Meal on Growth, Antioxidant Capacity, and Liver and Intestine Tissue Morphology of Bullfrog[Rana (Lithobates) catesbeiana][J]. Chinese Journal of Animal Nutrition, 2019 , 31(4) : 1864 -1876 . DOI: 10.3969/j.issn.1006-267x.2019.04.046

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