Effects of Dietary Oregano Essential Oil on Complex Stomach Development, Digestive Enzyme Activities and Ruminal Microflora of Sheep

ZHOU Rui, LANG Xia, WANG Cailian, LIU Lishan, WEI Sheng, SONG Shuzhen, WU Jianping

doi:10.3969/j.issn.1006-267x.2019.04.051

2019 , Vol. 31 >Issue 4: 1910 - 1918

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.04.051

Molecular Nutrition

Effects of Dietary Oregano Essential Oil on Complex Stomach Development, Digestive Enzyme Activities and Ruminal Microflora of Sheep

Expand

1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Animal Husbandry, Pasture and Green Agriculture Institute, Gansu Academy of Agricultural Sciences, Key Laboratory for Sheep, Gat and Cattle Germplasm and Straw Feed in Gansu Province, Lanzhou 730070, China

Received date: 2018-11-05

Online published: 2019-04-16

Fold

Abstract

This study aimed to investigate the effects of dietary oregano essential oil on complex stomach development, digestive enzyme activities and ruminal microflora of sheep. Eighteen 3-month-old crossbred (Suffolk×small tail Han sheep) F1 male lambs with similar body weight[(20.30±1.27) kg] and good body condition were randomly divided into 3 groups with 6 lambs per group. Sheep in the control group (CON group) were fed a basal diet, and those in the oregano essential oil groups were fed the basal diet supplemented with 4 (EO4 group) or 7 g/d (EO7 group) oregano essential oil. The experiment lasted for 72 days after 10 days adaption. The result showed as follows:1) the volume of rumen was significantly increased with the dietary oregano essential oil supplemental level increasing (P<0.05). Compared with the CON group, the volume index of rumen in EO7 group was increased by 2.09% (P<0.05), while the volume index of abmasum in EO7 group was decreased by 1.61% (P<0.05). 2) Compared with the CON group, the cellulase and pepsase activities of rumen in EO4 group were significantly increased (P<0.05), and the cellulase and β-glucanase activities of abomasum in EO4 group were significantly increased (P<0.05); the cellulase, β-glucanase and α-amylase activities of rumen and reticulum in EO7 group were significantly increased (P<0.05), and the cellulase activity of omasum and the pepsase activity of abomasum in EO7 group were significantly increased (P<0.05). 3) The population of protozao was significantly decreased by diet supplemented with 4 and 7 g/d oregano essential oil (P<0.05). Compared with the CON group, the population of rumen Ruminococcus flavefaciens in EO4 group was significantly increased (P<0.05); the populations of rumen fungi, Ruminococcus flavefaciens, Ruminococcus albus and Fibrobacter succinogenes in EO7 group were significantly increased (P<0.05). In conclusion, dietary oregano essential oil can advance the development of rumen and the proliferation of rumen fungi and fibrinolytic bacteria, and improve the digestive enzyme activities, while inhibit the growth of rumen protozoa. The effect is better when oregano essential oil supplemental level is 7 g/d.

Key words： sheep; oregano essential oil; complex stomach development; digestive enzyme activities; microflora

Cite this article

ZHOU Rui, LANG Xia, WANG Cailian, LIU Lishan, WEI Sheng, SONG Shuzhen, WU Jianping . Effects of Dietary Oregano Essential Oil on Complex Stomach Development, Digestive Enzyme Activities and Ruminal Microflora of Sheep[J]. Chinese Journal of Animal Nutrition, 2019 , 31(4) : 1910 -1918 . DOI: 10.3969/j.issn.1006-267x.2019.04.051

References

[1] PATRA A K,YU Z T.Essential oils affect populations of some rumen bacteria in vitro as revealed by microarray (Rumen Bact Array) analysis[J].Frontiers in Microbiology,2015,6:297.

[2] LANG G,BUCHBAUER G.A review on recent research results (2008-2010) on essential oils as antimicrobials and antifungals.A review[J].Flavour and Fragrance Journal,2012,27(1):13-39.

[3] BASER K H C.The turkish origanum species[M]//KINTZIOS S E.The genera origanum and lippia.New York:Taylor & Francis,2002:109-126.

[4] AMORATI R,FOTI M C,VALGIMIGLI L.Antioxidant activity of essential oils[J].Journal of Agricultural and Food Chemistry,2013,61(46):10835-10847.

[5] SIMITZIS P E.Enrichment of animal diets with essential oils-a great perspective on improving animal performance and quality characteristics of the derived products[J].Medicines,2017,4(2):35.

[6] SOULTOS N,TZIKAS Z,CHRISTAKI E,et al.The effect of dietary oregano essential oil on microbial growth of rabbit carcasses during refrigerated storage[J].Meat Science,2009,81(3):474-478.

[7] 梁建勇.不同添加剂对青贮饲料品质、纤维结构及绵羊瘤胃发酵的影响[D].硕士学位论文.兰州:甘肃农业大学,2016.

[8] 王娟,吴建平,焦婷,等.添加牛至精油对绵羊瘤胃发酵特征及青贮、黄贮玉米秸秆瘤胃降解率的影响[J].甘肃农业大学学报,2018,53(4):8-14.

[9] MUYZER G,DE WAAL E C,UITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and Environmental Microbiology,1993,59(3):695-700.

[10] 周瑞,赵生国,刘立山,等.饲粮中燕麦干草含量对绵羊瘤胃液pH及微生物区系的影响[J].动物营养学报,2016,28(5):1589-1597.

[11] KOIKE S,KOBAYASHI Y.Development and use of competitive PCR assays for the rumen cellulolytic bacteria:Fibrobacter succinogenes,Ruminococcus albus and Ruminococcus flavefaciens[J].FEMS Microbiology Letters,2001,204(2):361-366.

[12] KUMAR S,DAGAR S S,SIROHI S K,et al.Microbial profiles,in vitro gas production and dry matter digestibility based on various ratios of roughage to concentrate[J].Annals of Microbiology,2013,63(2):541-545.

[13] ANDERSON K L,NAGARAJA T G,MORRILL J L,et al.Ruminal microbial development in conventionally or early-weaned calves[J].Journal of Animal Science,1987,64(4):1215-1226.

[14] FLATT W P,WARNER R G,LOOSLI J K.Influence of purified materials on the development of the ruminant stomach[J].Journal of Dairy Science,1958,41(11):1593-1600.

[15] YANG B,HE B,WANG S S,et al.Early supplementation of starter pellets with alfalfa improves the performance of pre-and postweaning Hu lambs[J].Journal of Animal Science,2015,93(10):4984-4994.

[16] 陈昊,刘婷,吴建平,等.牛至精油对新生犊牛生长发育和血液免疫的影响[J].草业科学,2017,34(10):2141-2148.

[17] 姚喜喜,吴建平,刘婷,等.全混合日粮中添加牛至精油对泌乳期荷斯坦奶牛生产性能和蹄病发生率的影响[J].草业科学,2016,33(2):299-304.

[18] NOROUZIAN M A,VALIZADEH R.Effect of forage inclusion and particle size in diets of neonatal lambs on performance and rumen development[J].Journal of Animal Physiology and Animal Nutrition,2014,98(6):1095-1101.

[19] 郎侠.欧拉型藏羊消化系统发育和肌肉H-FABP基因表达规律的研究[D].博士学位论文.兰州:甘肃农业大学,2011.

[20] 王桂秋.营养水平对羔羊物质消化的影响及羔羊早期断奶时间的研究[D].硕士学位论文.北京:中国农业科学院,2005.

[21] 祁敏丽,刁其玉,张乃锋.羔羊瘤胃发育及其影响因素研究进展[J].中国畜牧杂志,2015,51(9):77-81.

[22] ?ITNAN R,BOMBA A,SOMMER A,et al.Development of rumen metabolism and ruminal epithelium in lambs[J].Archiv für Tierernaehrung,1993,44(3):227-233.

[23] 张宁.不同日龄小尾寒羊与滩羊消化器官及其胃消化酶活性的比较研究[D].硕士学位论文.杨凌:西北农林科技大学,2008.

[24] MOHARRERY A,DAS T K.Correlation between microbial enzyme activities in the rumen fluid of sheep under different treatments[J].Reproduction,Nutrition,Development,2001,41(6):513-529.

[25] KOIKE S,KOBAYASHI Y.Fibrolytic rumen bacteria:their ecology and functions[J].Asian-Australasian Journal of Animal Sciences,2009,22(1):131-138.

[26] 王雅倩,俞路,闫寒寒.瘤胃真菌对纤维降解的研究进展[J].饲料工业,2008,29(5):41-43.

[27] AGARWAL N,SHEKHAR C,KUMAR R,et al.Effect of peppermint (Mentha piperita) oil on in vitro methanogenesis and fermentation of feed with buffalo rumen liquor[J].Animal Feed Science and Technology,2009,148(2/3/4):321-327.

[28] PATRA A K,YU Z T.Effects of vanillin,quillaja saponin,and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen[J].Applied Microbiology and Biotechnology,2014,98(2):897-905.

[29] LIN B,LU Y,SALEM A Z M,et al.Effects of essential oil combinations on sheep ruminal fermentation and digestibility of a diet with fumarate included[J].Animal Feed Science and Technology,2013,184(1/2/3/4):24-32.

[30] MCINTOSH F M,WILLIAMS P,LOSA R,et al.Effects of essential oils on ruminal microorganisms and their protein metabolism[J].Applied and Environmental Microbiology,2003,69(8):5011-5014.

[31] THAO N T,WANAPAT M,CHERDTHONG A,et al.Effects of eucalyptus crude oils supplementation on rumen fermentation,microorganism and nutrient digestibility in swamp buffaloes[J].Asian-Australasian Journal of Animal Sciences,2014,27(1):46-54.

[32] FACIOLA A P,BRODERICK G A.Effects of feeding lauric acid or coconut oil on ruminal protozoa numbers,fermentation pattern,digestion,omasal nutrient flow,and milk production in dairy cows[J].Journal of Dairy Science,2014,97(8):5088-5100.

[33] NEWBOLD C J,MCINTOSH F M,WILLIAMS P,et al.Effects of a specific blend of essential oil compounds on rumen fermentation[J].Animal Feed Science and Technology,2004,114(1/2/3/4):105-112.

[34] PATRA A K,YU Z T.Effects of essential oils on methane production and fermentation by,and abundance and diversity of,rumen microbial populations[J].Applied and Environmental Microbiology,2012,78(12):4271-4280.

[35] ASH R W.Abomasal secretion and emptying in suckled calves[J].The Journal of Physiology,1964,172(3):425-438.

[36] WILLIAMS V J,ROY J H,GILLIES C M.Milk-substitute diet composition and abomasal secretion in the calf[J].British Journal of Nutrition,1976,36(3):317-335.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References