Effects of Synbiotics Substitution of Antibiotics on Growth Performance, Intestinal Mucosal Morphology, Immune Performance and Colonic Microbial Ecosystem of Weaned Piglets

WEN Xiaolu, WANG Li, YANG Xuefen, GAO Kaiguo, JIANG Zongyong, HU Youjun

doi:10.3969/j.issn.1006-267x.2019.05.015

2019 , Vol. 31 >Issue 5: 2088 - 2097

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.015

Molecular Nutrition

Effects of Synbiotics Substitution of Antibiotics on Growth Performance, Intestinal Mucosal Morphology, Immune Performance and Colonic Microbial Ecosystem of Weaned Piglets

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Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2018-10-15

Online published: 2019-05-15

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Abstract

The present study was conducted to investigate the effects of synbiotics substitution of antibiotics on growth performance, intestinal mucosal morphology, jejunal mucosal immune immunological cytokine contents and colon contents microbial composition of weaned piglets. A total of 72 Duroc×Landrace×Yorkshire weaned piglets were allotted randomly to 3 groups with 6 replicates and 4 piglets per replicate. The groups consisted of antibiotic group received diet supplemental antibiotics, and experiment groups were fed the diet supplemented with 500 (0.05%) and 1 000 mg/kg (0.10%) synbiotics, respectively. The whole trial lasted for 28 days, which was divided into two stages (days 1 to 14 and days 15 to 28). The results showed as follows: 1) there were no significant differences in average daily weight gain (ADG), average daily feed intake (ADFI), feed/gain (F/G) and diarrhea frequency in each stage among all groups (P>0.05), but there was an increase trend for ADG on days 1 to 14 of piglets in 0.05% synbiotics group compared with antibiotic group (P=0.07). 2) The villus height of jejunum in 0.05% and 0.10% synbiotics groups was significantly higher than that in antibiotic group (P<0.05), but there was no significant difference in crypt depth of duodenum, jejunum and ileum among all groups (P>0.05). 3) Compared with antibiotic group, the interleukin-4 (IL-4) content in jejunum mucosa of piglets in 0.05% and 0.10% synbiotics groups was significantly increased (P<0.05). The contents of tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in jejunum mucosa of piglets were not significantly different among all groups (P>0.05). 4) Compared with antibiotic group, the populations of Lactobacilli and Bifidobacterium in colonic contents of piglets in 0.05% synbiotics group were significantly increased (P<0.05), the population of Bifidobacterium in colonic contents of piglets in 0.10% synbiotics group was significantly increased (P<0.05). There were no significant differences in the populations of total bacteria and Escherichia coli in colonic contents of piglets among all groups (P>0.05). In conclusion, dietary synbiotics substitution of antibiotic not only does not decrease the growth performance of piglets, but also can control the diarrhea, increase the jejunal villus height, improve the immunity performance, and maintain the intestinal microbial ecosystem balance; the suitable dosage is 0.05%.

Key words： synbiotics; weaned piglets; growth performance; intestinal mucosal immune; intestinal mucosal morphology; intestinal microbial ecosystem

Cite this article

WEN Xiaolu, WANG Li, YANG Xuefen, GAO Kaiguo, JIANG Zongyong, HU Youjun . Effects of Synbiotics Substitution of Antibiotics on Growth Performance, Intestinal Mucosal Morphology, Immune Performance and Colonic Microbial Ecosystem of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2088 -2097 . DOI: 10.3969/j.issn.1006-267x.2019.05.015

References

[1] CAMPBELL J M,CRENSHAW J D,POLO J.The biological stress of early weaned piglets[J].Journal of Animal Science and Biotechnology,2013,4(1):19.

[2] WU Y P,JIANG Z Y,ZHENG C T,et al.Effects of protein sources and levels in antibiotic-free diets on diarrhea,intestinal morphology,and expression of tight junctions in weaned piglets[J].Animal Nutrition,2015,1(3):170-176.

[3] HEO J M,OPAPEJU F O,PLUSKE J R,et al.Gastrointestinal health and function in weaned pigs:a review of feeding strategies to control post-weaning diarrhea without using in-feed antimicrobial compounds[J].Journal of Animal Physiology and Animal Nutrition,2013,97(2):207-237.

[4] 李丽,刘哲.合生素对断奶仔猪生产性能、免疫功能和肠道菌群的影响[J].饲料研究,2013(8):57-60.

[5] GIBSON G R,ROBERFROID M B.Dietary modulation of the human colonic microbiota:introducing the concept of prebiotics[J].The Journal of Nutrition,1995,125(6):1401-1412.

[6] 谢全喜,亓秀晔,陈振,等.复合微生态制剂对断奶仔猪生长性能、腹泻率、免疫性能和肠道菌群的影响[J].动物营养学报,2017,29(3):850-858.

[7] 焦金真,王芃芃,汤少勋,等.浏阳黑山羊胃肠道不同部位重要功能微生物的数量分布特征研究[J].畜牧兽医学报,2013,44(10):1590-1599.

[8] 潘宝海,张建东,石现瑞,等.仔猪日粮中合生素替代抗生素的研究[J].饲料研究,2007(3):58-60.

[9] LIANG H Z,XIA S S,LIU C X,et al.Effects of synbiotics and antibiotics on nutrient digestibility and serum antioxidant function of weaned piglets[J].Animal Husbandry and Feed Science,2017,9(1):12-14,43.

[10] 张仕安,靳宇田.合生素在断奶仔猪上的饲喂效果[J].中国饲料,2015(4):31-34.

[11] SU Y T,CHEN X J,LIU M,et al.Effect of three lactobacilli with strain-specific activities on the growth performance,faecal microbiota and ileum mucosa proteomics of piglets[J].Journal of Animal Science and Biotechnology,2017,8:52.

[12] YI H B,WANG L,XIONG Y X,et al.Effects of Lactobacillus reuteri LR1 on the growth performance,intestinal morphology,and intestinal barrier function in weaned pigs[J].Journal of Animal Science,2018,96(6):2342-2351.

[13] LEE J S,AWJI E G,LEE S J,et al.Effect of Lactobacillus plantarum CJLP243 on the growth performance and cytokine response of weaning pigs challenged with enterotoxigenic Escherichia coli[J].Journal of Animal Science,2012,90(11):3709-3717.

[14] LIU H,JI H F,ZHANG D Y,et al.Effects of Lactobacillus brevis preparation on growth performance,fecal microflora and serum profile in weaned pigs[J].Livestock Science,2015,178:251-254.

[15] CHANG Y H,KIM J K,KIM H J,et al.Selection of a potential probiotic Lactobacillus strain and subsequent in vivo studies[J].Antonie Van Leeuwenhoek,2001,80(2):193-199.

[16] SHIM S B,VERSTEGEN M W A,KIM I H,et al.Effects of feeding antibiotic-free creep feed supplemented with oligofructose,probiotics or synbiotics to suckling piglets increases the preweaning weight gain and composition of intestinal microbiota[J].Archives of Animal Nutrition,2005,59(6):419-427.

[17] SWANSON K S,GRIESHOP C M,FLICKINGER E A,et al.Supplemental fructooligosaccharides and mannanoligosaccharides influence immune function,ileal and total tract nutrient digestibilities,microbial populations and concentrations of protein catabolites in the large bowel of dogs[J].The Journal of Nutrition,2002,132(5):980-989.

[18] OSAWA R,MATSUMOTO K.Digestion of staphylococcal enterotoxin by Bacillus natto[J]. Antonie van Leeuwenhoek, 1997, 71(4):307-311.

[19] 程群,姜淑贞,黄丽波,等.不同水平玉米赤霉烯酮对断奶母仔猪血清酶、代谢产物和肠道形态的影响[J].动物营养学报,2017,29(12):4578-4587.

[20] KERNÉIS S,BOGDANOVA A,KRAEHENBUHL J P,et al.Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria[J].Science,1997,277(5328):949-952.

[21] 陈凌锋,蔡旭滨,檀新珠,等.太子参茎叶多糖对断奶仔猪肠道免疫功能、肠黏膜形态结构及盲肠内容物菌群的影响[J].动物营养学报,2017,29(3):1012-1020.

[22] 赵峰,王波,夏中生,等.果寡糖和益生素制剂对断奶仔猪肠道健康的影响[J].黑龙江畜牧兽医,2018(6):137-141.

[23] GUERRA-ORDAZ A A,GONZÃLEZ-ORTIZ G,LA RAGIONE R M,et al.Lactulose and Lactobacillus plantarum,a potential complementary synbiotic to control postweaning colibacillosis in piglets[J].Applied and Environmental Microbiology,2014,80(16):4879-4886.

[24] SABATER-MOLINA M, LARQUÉ L,TORRELLA F,et al.Effects of fructooligosaccharides on cecum polyamine concentration and gut maturation in early-weaned piglets[J].Journal of Clinical Biochemistry and Nutrition,2011,48(3):230-236.

[25] 赵天智,周玉照,李灿荣,等.饲粮添加益生菌和低聚果糖对育肥猪生长性能、肠道菌群和肠道形态的影响[J].中国饲料,2018(4):45-49.

[26] 张锦华,王萍,何后军,等.纳豆提取物和纳豆菌培养液对仔猪肠道菌体外抑菌试验[J].江西农业大学学报,2007,29(2):262-265.

[27] PAN X D,CHEN F Q,WU T X,et al.Prebiotic oligosaccharides change the concentrations of short-chain fatty acids and the microbial population of mouse bowel[J].Journal of Zhejiang University, Science B:Biomedicine & Biotechnology,2009,10(4):258-263.

[28] TSUKAHARA T,IWASAKI Y,NAKAYAMA K,et al.Stimulation of butyrate production in the large intestine of weaning piglets by dietary fructooligosaccharides and its influence on the histological variables of the large intestinal mucosa[J].Journal of Nutritional Science and Vitaminology,2003,49(6):414-421.

[29] YANG K M,JIANG Z Y,ZHENG C T,et al.Effect of Lactobacillus plantarum on diarrhea and intestinal barrier function of young piglets challenged with enterotoxigenic Escherichia coli K88[J].Journal of Animal Science,2014,92(4):1496-1503.

[30] 孙丽华,蒋宗勇,林映才,等.早期断奶仔猪肠黏膜免疫功能研究进展[J].东北农业大学学报,2009,40(1):138-144.

[31] COLLART M A,BAEUERLE P,VASSALLI P.Regulation of tumor necrosis factor alpha transcription in macrophages:involvement of four κB-like motifs and of constitutive and inducible forms of NF-κB[J].Molecular and Cellular Biology,1990,10(4):1498-1506.

[32] LIBERMANN T A,BALTIMORE D.Activation of interleukin-6 gene expression through the NF-κB transcription factor[J].Molecular and Cellular Biology,1990,10(5):2327-2334.

[33] AL-SADI R,BOIVIN M,MA T.Mechanism of cytokine modulation of epithelial tight junction barrier[J].Frontiers in Bioscience,2009,14(7):2765-2778.

[34] ZARZAUR B L,WU Y,FUKATSU K,et al.The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice[J].Surgery,2002,131(1):59-65.

[35] DONG X,ZHANG N,ZHOU M,et al.Effects of dietary probiotics on growth performance,faecal microbiota and serum profiles in weaned piglets[J].Animal Production Science,2014,54(5):616-622.

[36] LE BOURGOT C,LE NORMAND L,FORMAL M,et al.Maternal short-chain fructo-oligosaccharide supplementation increases intestinal cytokine secretion,goblet cell number,butyrate concentration and Lawsonia intracellularis humoral vaccine response in weaned pigs[J].British Journal of Nutrition,2017,117(1):83-92.

[37] LÄHTEINEN T,RINTTILÄ T,KOORT J M K,et al.Effect of a multispecies Lactobacillus formulation as a feeding supplement on the performance and immune function of piglets[J].Livestock Science,2015,180:164-171.

[38] FINK L N,FRØKIAER H.Dendritic cells from Peyer's patches and mesenteric lymph nodes differ from spleen dendritic cells in their response to commensal gut bacteria[J].Scandinavian Journal of Immunology,2008,68(3):270-279.

[39] DONG H L,ROWLAND I,YAQOOB P.Comparative effects of six probiotic strains on immune function in vitro[J].British Journal of Nutrition,2012,108(3):459-470.

[40] KIM C H,PARK J,KIM M.Gut microbiota-derived short-chain fatty acids,T cells,and inflammation[J].Immune Network,2014,14(6):277-288.

[41] 金燕飞.饲用纳豆芽孢杆菌微生态制剂的研制与开发[D].硕士学位论文.杭州:浙江大学,2006.

[42] BOMBA A,NEMCOVÁ R,GANCARCÍKOVÁ S,et al.Improvement of the probiotic effect of micro-organisms by their combination with maltodextrins,fructo-oligosaccharides and polyunsaturated fatty acids[J].British Journal of Nutrition,2002,88(Suppl.1):S95-S99.

[43] 杭苏琴,时祺,丁立人,等.果寡糖对断奶前仔猪胃肠道组织形态、消化酶、有机酸及乳酸杆菌菌群的影响[J].草业学报,2014,23(2):260-267.

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