Effects of Folate Supplemental Level on Expression and Methylation of Lipid Metabolism Related Genes in Liver of Broilers

XING Jinyi, ZHANG Yujie, ZHANG Ningbo, JING Wenqian, LI Zhu, WANG Xinzheng, XU Yunchao

doi:10.3969/j.issn.1006-267x.2019.05.016

2019 , Vol. 31 >Issue 5: 2098 - 2106

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.016

Molecular Nutrition

Effects of Folate Supplemental Level on Expression and Methylation of Lipid Metabolism Related Genes in Liver of Broilers

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1. School of Life Sciences, Linyi University, Linyi 276005, China;
2. College of Agriculture and Forestry Sciences, Linyi 276005, China

Received date: 2018-10-28

Online published: 2019-05-15

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Abstract

The aims of this study were to investigate the effects of different folate supplemental levels on the activities of lipoprotein lipase (LPL), fatty acid synthase (FAS) and peroxisome proliferator-activated receptor gamma (PPARγ) content in serum and the expression and methylation of lipid metabolism related genes in liver of broilers. A total of 300 one-day-old Arbor Acres (AA) broilers were randomly allotted to 3 groups with 5 replicates of 20 broilers each. Broilers in control group were fed a basal diet, and broilers in trial groups were fed the basal diet supplemented with 5 and 10 mg/kg folate, respectively. The experiment lasted for 42 days. The results showed as follows: 1) compared with the control group, diet supplemented with 10 mg/kg folate significantly reduced the activity of LPL in serum of 21-day-old broilers (P<0.05); diet supplemented with 5 and 10 mg/kg folate significantly reduced the activity of LPL in serum of 42-day-old broilers (P<0.01), significantly increased the content of PPARγ in serum of 21- and 42-day-old broilers (P<0.01), and significantly reduced the activity of FAS in serum of 21- and 42-day-old broilers (P<0.01). 2) The results of real-time quantitative PCR indicated that the relative expression levels of LPL and PPARγ genes in liver of 42-day-old broilers were not significantly affected by diet supplemented with 5 and 10 mg/kg folate (P>0.05). Compared with the control group, the relative expression levels of FAS gene in liver of 42-day-old broilers was significantly decreased by diet supplemented with 5 and 10 mg/kg folate (P<0.05). 3) The results of methylation analysis showed that the methylation ratios of LPL and PPARγ genes in liver of 42-day-old broilers were not significantly affected by diet supplemented with 5 and 10 mg/kg folate (P>0.05). Compared with the control group, diet supplemented with 5 mg/kg folate significantly improved the methylation ratio of FAS gene in liver of 42-day-old broilers (P<0.01). In conclusion, diet supplemented with 5 and 10 mg/kg folate can significantly decrease serum LPL and FAS activities, significantly increase serum PPARγ content, significantly reduce the expression of FAS gene in liver of 42-day-old broilers. Moreover, diets supplemented with 5 mg/kg folate can also significantly increase the methylation level of FAS gene in liver of 42-day-old broilers.

Key words： broilers; folate; methylation; lipoprotein lipase; peroxisome proliferator-activated receptor gamma; fatty acid synthase

Cite this article

XING Jinyi, ZHANG Yujie, ZHANG Ningbo, JING Wenqian, LI Zhu, WANG Xinzheng, XU Yunchao . Effects of Folate Supplemental Level on Expression and Methylation of Lipid Metabolism Related Genes in Liver of Broilers[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2098 -2106 . DOI: 10.3969/j.issn.1006-267x.2019.05.016

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