Molecular Nutrition

Effects of Bacillus subtilis Supplemented in Low Fish Meal Diets on Growth Performance, Non-Specific Immunity and Disease Resistance of Juvenile Litopenaeus vannamei

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  • 1. Aquatic Animal Nutrition and Feed Laboratory, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
    2. Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang 524074, China

Received date: 2018-10-22

  Online published: 2019-05-15

Abstract

The effects of dietary Bacillus subtilis on growth performance, serum non-specific immune enzyme activities, hepatopancreas non-specific immune enzyme mRNA expression and disease resistance of juvenile Litopeneaus vannamei under low fish meal diet were studied in this experiment. Eight hundred and forty healthy juvenile Litopeneaus vannamei with an average body weight of (0.40±0.01) g were randomly divided into 7 groups with 3 replicates per group and 30 fish per replicate. The shrimp in the 7 group were fed a low fish meal diet (contained 10% fish meal) and supplemented with Bacillus subtilis (the viable count was 1.0×108 CFU/mL) of 0 (control), 0.10%, 0.25%, 0.50%, 0.75%, 1.00% and 1.25%, respectively. The experiment lasted for 8 weeks. The results showed that the weight gain rate (WGR) and specific growth rate (SGR) in 0.10% group was not significantly different compared with control group (P>0.05), but they were in other groups were significantly higher than those in control group (P<0.05). The feed conversion ratio (FCR) reached the lowest at the 0.75% supplemental level, which was significantly lower than that in other groups (P<0.05), but the change tendency of protein efficiency ratio (PER) was contrary to the FCR. The activities of acid phosphatase(ACP), catalase (CAT), phenol oxide (PO) and lysozyme (LZM) in serum increased firstly and then decreased with the Bacillus subtilis supplemental level increasing, and they had the highest values at 1.00%, 1.00%, 0.50% and 0.75% supplemental levels, respectively. The activities of alkaline phosphatase (AKP) and superoxide dismutase (SOD) in serum in supplemental groups were significantly higher than those in control group (P<0.05), and both of them had the highest values in 0.75% group. The mRNA relative expression level of CAT in hepatopancreas in supplemental groups was significantly higher than that in control group (P<0.05), and the mRNA relative expression level of LZM in hepatopancreas in 0.50%, 0.75%, 1.00% and 1.50% groups was significantly higher than that in control group (P<0.05). The cumulative survival rate after 96 h challenged with Vibrio harveyi increased firstly and then trended to stable with the Bacillus subtilis supplemental level increasing, and it in 0.50%, 0.75%, 1.00% and 1.50% groups was significantly higher than that in control group (P<0.05), but it in 0.10% and 0.25% groups had no significant difference compared with control group (P>0.05). It is concluded that the suitable amount of Bacillus subtilis adding in the low fish meal diet can promote the growth of Litopeneaus vannamei and increase its non-specific immunity and disease resistance. On the basis of WGR, according to the broken line model, the growth promoting effect is the best when adding 0.73% Bacillus subtilis (the viable count is 1.0×108 CFU/mL) in the low fish meal diet (contained 10% fish meal).

Cite this article

LI Junliang, YANG Qihui, TAN Beiping, DONG Xiaohui, CHI Shuyan, LIU Hongyu, ZHANG Shuang, ZHANG Haitao . Effects of Bacillus subtilis Supplemented in Low Fish Meal Diets on Growth Performance, Non-Specific Immunity and Disease Resistance of Juvenile Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2212 -2221 . DOI: 10.3969/j.issn.1006-267x.2019.05.028

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