Influences of Bacillus subtilis Copper on Growth and Development, Organ Indexes, Copper Deposition and Serum Biochemical and Antioxidant Indices of Congenital Copper Deficiency Rats

LIU Guodong, WANG Baowei, HUANG Yanping, GE Wenhua, ZHANG Ming'ai, YUE Bin, GAO Kai, KONG Min

doi:10.3969/j.issn.1006-267x.2019.05.030

2019 , Vol. 31 >Issue 5: 2232 - 2242

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.030

Molecular Nutrition

Influences of Bacillus subtilis Copper on Growth and Development, Organ Indexes, Copper Deposition and Serum Biochemical and Antioxidant Indices of Congenital Copper Deficiency Rats

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1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
2. National Waterfowl Industrial Technology System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2018-10-15

Online published: 2019-05-15

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Abstract

This experiment was conducted to study the influences of Bacillus subtilis copper on growth and development, organ indices, copper deposition and serum biochemical and antioxidant parameters of congenital copper deficiency rats. The experiment was divided into model period and intervention period. In the model period, seventy-two pregnant SD rats were divided into 2 groups (model group and control group) with 6 replicates per group and 6 replicates per replicate. The pregnancy rats in the model group were fed a low copper diet (copper content was 0.33 mg/kg), and the others in the control group were fed a normal diet (low copper diets supplemented with 6.0 mg/kg copper sulfate pentahydrate), continue until the end of the lactation period and to verify that the model was successful. In the intervention period, a total of 108 successfully modeled copper deficiency young rats were randomly divided into 6 groups, groups Ⅰ to Ⅲ were copper sulfate groups (low copper diets supplemented with 3.0, 6.0 and 9.0 mg/kg copper sulfate pentahydrate, respectively), and groups Ⅳ to Ⅵ were Bacillus subtilis copper groups (low copper diets supplemented with 3.0, 6.0 and 9.0 mg/kg Bacillus subtilis copper, respectively), each group had 3 replicates and each replicate had 6 rates. The experiment lasted for 35 days. The results showed as follows: 1) under the same copper addition condition, the body weight and activities of glutamic-pyruvic transaminase (ALT) and glutathione peroxidase (GSH-Px) in serum of group Ⅳ were significantly higher than those of group Ⅰ (P < 0.05 or P < 0.01), and the serum glutamic-oxaloacetic transaminase/glutamic-pyruvic transaminase (AST/ALT) of group Ⅳ was significantly lower than that of group Ⅰ (P < 0.05). The activities of ceruloplasmin (CP) and alkaline phosphatase (AKP) in serum of group Ⅴ were significantly higher than those of group Ⅱ (P < 0.05 or P < 0.01), and the serum malonaldehyde (MDA) content of group Ⅴ was significantly lower than that of group Ⅱ (P < 0.05). The serum AKP activity and kidney copper deposition of group Ⅵ were significantly higher than those of group Ⅲ (P < 0.05 or P < 0.01). 2) In copper sulfate groups, the body weight and body length of groups Ⅱ and Ⅲ were significantly higher than those of group Ⅰ (P < 0.05), the serum CP activity of group Ⅲ was significantly lower than that of groups Ⅰ and Ⅱ (P < 0.05 or P < 0.01), the serum AKP activity of group Ⅲ was significantly lower than that of group Ⅱ (P < 0.05), the activities of glutamic-oxaloacetic transaminase (AST), GSH-Px and Cu-Zn superoxide dismutase (CuZn-SOD) in serum and kidney copper deposition of group Ⅲ were significantly higher than those of group Ⅰ (P < 0.05 or P < 0.01), and the serum MDA content of group Ⅲ was significantly lower than that of group Ⅰ (P < 0.05). In Bacillus subtilis copper groups, the body weight, body length and the activities of AST, GSH-Px and CuZn-SOD in serum and the copper deposition in heart, liver and kidney of group Ⅵ were significantly higher than those of group Ⅳ (P < 0.05 or P < 0.01), the activities of CP and AKP in serum of group Ⅵ were significantly higher than those of groups Ⅳ and Ⅴ (P < 0.05 or P < 0.01). In conclusion, the Bacillus subtilis copper can increase the body weight and the activities of CP, AKP and ALT in serum of congenital copper deficiency rats, improve the body antioxidant capacity, increase the copper deposition in liver and kidney, the effects are better than copper sulfate, and reduce the copper addition in the diet.

Key words： Bacillus subtilis copper; rats; congenital copper deficiency; growth and development; serum biochemical parameters; antioxidant; copper deposition

Cite this article

LIU Guodong, WANG Baowei, HUANG Yanping, GE Wenhua, ZHANG Ming'ai, YUE Bin, GAO Kai, KONG Min . Influences of Bacillus subtilis Copper on Growth and Development, Organ Indexes, Copper Deposition and Serum Biochemical and Antioxidant Indices of Congenital Copper Deficiency Rats[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2232 -2242 . DOI: 10.3969/j.issn.1006-267x.2019.05.030

References

[1] GAETKE L M,CHOW C K.Copper toxicity,oxidative stress,and antioxidant nutrients[J].Toxicology,2003,189(1/2):147-163.

[2] KIM B E,NEVITT T,THIELE D J.Mechanisms for copper acquisition,distribution and regulation[J].Nature Chemical Biology,2008,4(3):176-185.

[3] JU C F,YU B,ZHU D,et al.The Effect of dietary sulphate copper supplementation on growth performance,intestinal digestive enzymes and absorptive transporters in weanling pigs[J].Journal of Animal Science and Biotechnology,2010(1):35-43.

[4] KUMAR V,KALITA J,BORA H K,et al.Temporal kinetics of organ damage in copper toxicity:a histopathological correlation in rat model[J].Regulatory Toxicology and Pharmacology,2016,81:372-380.

[5] 王聪,董宽虎,刘强,等.蛋氨酸铜对西门塔尔牛日粮养分消化代谢及血液指标的影响[J].中国生态农业学报,2008,16(6):1523-1527.

[6] HUANG Y,YOO J S,KIM H J,et al.The effects of different copper (inorganic and organic) and energy (tallow and glycerol) sources on growth performance,nutrient digestibility,and fecal excretion profiles in growing pigs[J].Asian-Australasian Journal of Animal Sciences,2010,23(5):573-579.

[7] 刘冬,黄守敏,毕璋友,等.益生菌混合培养富集锌条件优化研究[J].安徽农业科学,2015,43(20):259-262.

[8] REEVES P G,NIELSEN F H,FAHEY G C,Jr.AIN-93 purified diets for laboratory rodents:final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J].The Journal of Nutrition,1993,123(11):1939-1951.

[9] 吕咸坤.家畜缺乏微量元素铜、锌、锰的症状及防治[J].饲料博览,2017(5):58.

[10] ERGAZ Z,SHOSHANI-DROR D,GUILLEMIN C,et al.The effect of copper deficiency on fetal growth and liver anti-oxidant capacity in the Cohen diabetic rat model[J].Toxicology and Applied Pharmacology,2012,265(2):209-220.

[11] DZIEZYC K,LITWIN T,SOBA?SKA A,et al.Symptomatic copper deficiency in three wilson's disease patients treated with zinc sulphate[J].Neurologiai Neurochirurgia Polska,2014,48(3):214-218.

[12] 胥彩玉,胥彩霞,刘国华,等.有机与无机微量元素比例对肉仔鸡生长和组织生化指标的影响[J].动物营养学报,2015,27(5):1549-1558.

[13] 赵春雨,张天芮,刘博,等.日粮铜来源及水平对仔猪生长性能及组织铜含量的影响[J].家畜生态学报,2015,36(9):46-50.

[14] 张名爱,杨文娇,张泽楠,等.饲粮添加枯草芽孢杆菌对5-16周龄五龙鹅肠道发育、微生物菌群结构及血清酶活性的影响[J].动物营养学报,2017,29(9):3175-3183.

[15] SMITH M S.Responses of chicks to dietary supplements of copper sulphate[J].British Poultry Science,1969,10(2):97-108.

[16] 田相迪,朱风华,孙金全,等.不同剂量纳米氧化铜对肉仔鸡生长性能的影响[J].饲料研究,2007(7):41-44.

[17] 郑鑫,刘国文,王哲,等.铜对猪原代培养肝细胞中铜蓝蛋白酶活性的影响[J].饲料与畜牧,2006(3):13-16.

[18] 赵春雨,张天芮,刘博,等.日粮铜来源及水平对猪血液生化指标及含铜酶活性的影响[J].中国畜牧杂志,2015,51(21):39-44.

[19] 钱剑,刘国文,王哲,等.铜对软骨细胞碱性磷酸酶活性影响的实验观察[J].中国兽医杂志,2005,41(3):21-22.

[20] BOAL A K,ROSENZWEIG A C.Structural biology of copper trafficking[J].Chemical Reviews,2009,109(10):4760-4779.

[21] YAN Q,XIE S,ZHU X,et al.Dietary methionine requirement for juvenile rockfish,Sebastes schlegeli[J].Aquaculture Nutrition,2007,13(3):163-169.

[22] LIN L,ZENG X L,ZHANG J.Effect of profenofos poisoning on liver lipid peroxidation and liver function in rabbits[J].Chinese Journal of Clinical Rehabilitation,2004,8(21):4380-4381.

[23] 谭志刚,王墙,林滋,等.铜过载对小鼠肝肾的损伤[J].畜牧与兽医,2015,47(7):88-90.

[24] 李万立,罗海吉,查龙应.铜过量小鼠肝损伤模型的建立及各项指标的观察研究[J].热带医学杂志,2008,8(12):1210-1212.

[25] 冯杰,刘欣,吴新民,等.酪蛋白铜对仔猪生长及血清铜蓝蛋白和SOD活性的影响[J].中国畜牧杂志,2005,41(3):14-17.

[26] PAYNTER D I.The role of dietary copper,manganese,selenium,and vitamin E in lipid peroxidation in tissues of the rat[J].Biological Trace Element Research,1980,2(2):121-135.

[27] O'DONOHUE J W,REID M A,VARGHESE A,et al.Micronodular cirrhosis and acute liver failure due to chronic copper self-intoxication[J].European Journal of Gastroenterology & Hepatology,1993,5(7):561-562.

[28] ARAYA M,OLIVARES M,PIZARRO F,et al.Copper exposure and potential biomarkers of copper metabolism[J].Biometals,2003,16(1):199-204.

[29] CHOLEWINSKAÁ E,JUSKIEWICZ J,OGNIK K.Comparison of the effect of dietary copper nanoparticles and one copper (Ⅱ) salt on the metabolic and immune status in a rat model[J].Journal of Trace Elements in Medicine and Biology,2018,48:111-117.

[30] OZKUL H,KIRKPINAR F,MERT S,et al.Effects of high levels of dietary copper sulfate and copper proteinate on growth performance,retention for copper and zinc of rats[J].Journal of Animal and Veterinary Advances,2011,10(11):1373-1377.

[31] ENGLE T E,SPEARS J W.Effects of dietary copper concentration and source on performance and copper status of growing and finishing steers[J].Journal of Animal Science,2000,78(9):2446-2451.

[32] 潘娜,朱风华,王友令,等.日粮添加高水平硫酸锌和纳米氧化铜对鸡脏器组织锌铜沉积的影响[J].中国农业科学,2011,44(23):4874-4881.

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