Effects of Bacillus subtilis-Zinc on Immunity Function, Antioxidant Capacity and Reproduction Organ Development of Congenital Zinc Deficiency Rats

HUANG Yanping, WANG Baowei, LIU Guodong, GE Wenhua, ZHANG Mingai, YUE Bin, WANG Wenjie

doi:10.3969/j.issn.1006-267x.2019.05.031

2019 , Vol. 31 >Issue 5: 2243 - 2253

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.031

Molecular Nutrition

Effects of Bacillus subtilis-Zinc on Immunity Function, Antioxidant Capacity and Reproduction Organ Development of Congenital Zinc Deficiency Rats

Expand

1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
2. National Waterfowl Industrial Technology System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2018-10-31

Online published: 2019-05-15

Fold

Abstract

Bacillus subtilis-zinc was used as a material in this study, and the effects of it on immunity function, antioxidant capacity and reproductive organ development of congenital zinc deficiency rats were investigated. The trial was divided into two stages: establishment of congenital zinc deficiency model and the intervention stage of zinc-deficient rats. Model establishment stage: a total of 72 SD rats in the same late pregnancy period were selected and divided into zinc deficiency group and control group with 6 replicates per group and 6 rats per replicate. The rats in the zinc deficiency group were fed a low-zinc diet (zinc content was 13 mg/kg) from 10 days after pregnancy, and those in the control group were fed a normal diet (zinc content was 38 mg/kg), until the offspring rats were 24 days of age. After that, intervention trial stage: a total of 90 rats with congenital zinc deficiency were randomly divided into 5 groups: zinc deficiency group, ZnSO₄ group and low, medium and high doses of Bacillus subtilis-zinc groups, all rats were fed low-zinc diets; 18 offspring rats from the control group were randomly selected and used as a control group to feed the normal diet. Each group had 3 replicates, 6 rats in each replicate. The experiment lasted for 5 weeks. The results showed as follows: 1) the medium and high dose of Bacillus subtilis-zinc could promote the development the kidney and ovary of congenital zinc deficiency rats, increase the contents of immunoglobulin M (IgM), monocyte chemoattractant protein-1 (MCP-1) and the activites of zinc superoxide dismutase (Cu-Zn SOD), glutathione peroxidase (GSH-Px) in serum, contribute to the maturation of follicles, the secretion of sex hormones such as estradiol (E₂), follicle-stimulating hormone (FSH) and luteinizing hormone (LH). 2) The medium-dose of Bacillus subtilis-zinc was superior to inorganic zinc in promoting liver and ovary organ development, improving immune function and antioxidant capacity in rats with congenital zinc deficiency. Bacillus subtilis-zinc can promote the development of liver, ovary and other organs in rats with congenital zinc deficiency, improve immune function and antioxidant capacity, thereby promoting the repair of physiological functions of zinc-deficient rats, and its intervention effect is better than the equivalent zinc content of ZnSO₄, and can reduce the amount of zinc added to the diet.

Key words： Bacillus subtilis-zinc; rats; congenital zinc deficiency; immunity function; antioxidant capacity; reproductive organ development

Cite this article

HUANG Yanping, WANG Baowei, LIU Guodong, GE Wenhua, ZHANG Mingai, YUE Bin, WANG Wenjie . Effects of Bacillus subtilis-Zinc on Immunity Function, Antioxidant Capacity and Reproduction Organ Development of Congenital Zinc Deficiency Rats[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2243 -2253 . DOI: 10.3969/j.issn.1006-267x.2019.05.031

References

[1] CHIMIENTI F,AOUFFEN M,FAVIER A,et al.Zinc homeostasis-regulating proteins:new drug targets for triggering cell fate[J].Current Drug Targets,2003,4(4):323-338.

[2] 安利峰,胜利,范桂香.锌与生殖健康[J].国外医学(医学地理分册),2004,25(1):14-16.

[3] 刘浩,成小松,崔美芝,等.缺锌对大鼠生殖功能影响的实验研究[J].中国地方病学杂志,2003,22(4):383.

[4] 赵佳英,康德灿,高永峰,等.微生物富集有益元素食品的研发概况与展望[J].食品研究与开发,2017,38(12):206-210.

[5] LI W F,ZHOU X X,LU P.Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis[J].Research in Microbiology,2004,155(8):605-610.

[6] WHITE C L.The effect of zinc deficiency on the body composition of rats[J].Biological Trace Element Research,1988,17(1):175-187.

[7] 钱立群,金艳凤,刘凤莲,等.锌对大鼠生长发育和抗氧化作用的影响[J].环境与健康杂志,2006,23(3):217-220.

[8] 郭美荣,金艳凤,钱立群.锌对大鼠生长发育影响的研究[J].旅行医学科学,2007,13(1):5-9.

[9] 陈丽敏,王玉.维生素A和锌过量摄入对雌性大鼠生殖系统的影响[J].现代预防医学,2010,37(12):2309-2311.

[10] DARDENNE M.Zinc and immune function[J].European Journal of Clinical Nutrition,2002,56:S20-S23.

[11] 周立波,孙淑滨,景慎文.缺锌对孕鼠生长发育及其肝脏、胎盘中谷胱甘肽过氧化物酶及过氧化氢酶活性的影响[J].山东医药,2002,42(36):1-3.

[12] 张伟庆,黄福佳,杨萧华,等.枯草芽孢杆菌工程菌(WB800N-CC31)对大鼠免疫指标的影响[J].黑龙江畜牧兽医,2017(10):184-186.

[13] 边羽芊.浅析枯草芽孢杆菌研究进展与应用[J].科学技术创新,2017(30):33-34.

[14] REEVES P G,NIELSEN F H,FAHEY G C.AIN-93 purified diets for laboratory rodents:final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J].The Journal of Nutrition,1993,123(11):1939-1951.

[15] EL HENDY H A,YOUSEF M I,ABO EL-NAGA N I.Effect of dietary zinc deficiency on hematological and biochemical parameters and concentrations of zinc,copper,and iron in growing rats[J].Toxicology,2001,167(2):163-170.

[16] SUN J Y,JING M Y,WENG X Y,et al.Effects of dietary zinc levels on the activities of enzymes,weights of organs,and the concentrations of zinc and copper in growing rats[J].Biological Trace Element Research,2005,107(2):153-165.

[17] SWINKELS J W G M,KORNEGAY E T,ZHOU W,et al.Effectiveness of a zinc amino acid chelate and zinc sulfate in restoring serum and soft tissue zinc concentrations when fed to zinc-depleted pigs[J].Journal of Animal Science,1996,74(10):2420-2430.

[18] 邝声耀,唐凌,张纯,等.有机锌在动物营养中的研究与应用[J].中国畜牧兽医,2006,33(7):18-21.

[19] YADRICK M K,KENNEY M A,WINTERFELDT E A.Iron,copper,and zinc status:response to supplementation with zinc or zinc and iron in adult females[J].American Journal of Clinical Nutrition,1989,49(1):145-150.

[20] 罗治彬,吴嘉惠,史景泉,等.高锌对大鼠肠黏膜免疫的影响[J].中国药理学与毒理学杂志,2000,14(1):36-39.

[21] 王成贵.生物锌与有机锌对幼儿生长发育影响的研究[J].中国热带医学,2009,9(3):580-581,468.

[22] 郝洋洋,张修修,王玉,等.有机锌和有机锰对蛋种母鸡生产性能、蛋品质、抗氧化能力和免疫功能的影响[J].动物营养学报,2018,30(12):4931-4938.

[23] VERMA P C,GUPTA R P,SADANA J R,et al.Effect of experimental zinc deficiency and repletion on some immunological variables in guinea-pigs[J].British Journal of Nutrition,1988,59(1):149-154.

[24] AHN S H,UM J S,KIM D H,et al.Effects of the sources and levels of supplemental zinc on the performance of weanling pigs[J].Korean Journal of Animal Science,1998,40(1):9-12.

[25] 王福明.氨基酸螯合锌对建昌鸭生长性能和免疫功能的影响[J].湖北农业科学,2014,53(5):1117-1119.

[26] SOUTHERN L L,BAKER D H.Zinc toxicity,zinc deficiency and zinc-copper interrelationship in Eimeria acervulina-infected chicks[J].Journal of Nutrition,1983,113(3):688-696.

[27] 王慧,张霞,辛蕊华,等.酵母锌对肉鸡生长性能及生理功能的影响[J].中国兽医学报,2012,32(3):488-492.

[28] 张莉,池志宏,王月,等.硒酸锌金属自显影技术检测游离锌离子在小鼠卵巢的分布[J].解剖科学进展,2007,13(4):346-347,352.

[29] 刘凤霞,刘幸,齐茜,等.日粮有机锌水平对产蛋后期蛋鸡生产性能、免疫功能和抗氧化指标的影响[J].中国家禽,2017,39(24):28-34.

[30] 辛华玲,韩同喜,龚书明,等.锌与锗对小鼠免疫功能及抗氧化作用的实验研究[J].中华预防医学杂志,1996,30(4):221-224.

[31] EGWURUGWU J N,IFEDI C U,UCHEFUNA R C,et al.Effects of zinc on male sex hormones and semen quality in rats[J].Nigerian Journal of Physiological Sciences,2013,28(1):17-22.

[32] GILABERT E R,RUIZ E,OSORIO C,et al.Effect of dietary zinc deficiency on reproductive function in male rats:biochemical and morphometric parameters[J].The Journal of Nutritional Biochemistry,1996,7(7):403-407.

[33] 梁建光.奶牛的有机锌应用效果研究进展[J].乳业科学与技术,2007,30(6):274-277.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References