Molecular Nutrition

Effects of Fructus Corni Fruit Core on Lactation Performance, Milk Fatty Acid Composition and Expression of Lipid Metabolism Related Genes in Mammary Gland of Dairy Goats

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  • 1. College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Henan Key Laboratory of Innovative Utilization for Unconventional Feed Resources, International Joint Laboratory of Ruminant Nutrition and Feed Resources Development in Henan Province, Zhengzhou 450046, China;
    2. College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling 712100, China

Received date: 2018-10-30

  Online published: 2019-05-15

Abstract

The aim of this study was to investigate the effects of adding fructus corni fruit core on lactation performance, milk fatty acid composition and expression of lipid metabolism related genes in mammary gland of dairy goats. Fifty dairy goats with two parities, similar lactation days[(20±7) days]and similar body weight were selected and randomly divided into two groups, and the goats in the two groups were fed a basal diet (control group) and the basal diet+1% fructus corni fruit core (trial group), respectively. Each group had five replicates with five goats per replicate. The duration of preliminary experiment and formal experiment were 10 days and 45 days, respectively. Milk yield was measured during the experiment. At the end of the experiment, blood samples were collected from jugular vein for serum biochemical indexes analysis. Milk samples were collected for measuring milk composition. In addition, milk and mammary gland samples were collected, and the fatty acid composition in goat milk and the expression of lipid metabolism related genes in the mammary gland were determined by gas chromatography and real time quantitative PCR, respectively. The results showed as follows: 1) the aspartate aminotransferase (AST) activity, total protein (TP)and albumin (ALB) contents in serum of the trial group were significantly increased compared with the control group (P<0.05 or P<0.01), but there were no significant changes in other biochemical indexes in serum (P>0.05). 2) The milk yield of the trial group was significantly higher than that of the control group (P<0.05). Compared with the control group, the milk fat percentage and milk solids content of the trial group were significantly decreased (P<0.01), while the milk protein percentage and lactose percentage did not significantly change (P>0.05). 3) The contents of polyunsaturated fatty acid C18:3n6, short and medium-chain fatty acids C4:0, C6:0, C8:0 and C10:0, and saturated fatty acids C14:0, C16:0 and C20:0 in goat milk of the trial group were significantly higher than those of the control group (P<0.05), while the content of monounsaturated fatty acid C15:1 was significantly lower than that of the control group (P<0.05). 4) Compared with the control group, the addition of fructus corni fruit core significantly up-regulated the relative expression levels of acetyl-CoA carboxylase (ACC) and adipose triglyceride lipase (ATGL) in the mammary gland (P<0.05), and significantly down-regulated the relative expression levels of stearoyl-CoA desaturase 1 (SCD1) (P<0.05), but had no significant effects on the relative expression levels of diacylglycerol acyltransferase 2 (DGAT2), fatty acid synthase (FASN), sterol regulatory element-binding protein 1 (SREBP1) and elongase of very long chain fatty acid 6 (ELOVL6) (P>0.05). In conclusion, the supplementation of fructus corni fruit core can increase milk yield of dairy goats, but it significantly inhibits the milk fat percentage. Fructus corni can regulate the expression of ACC, SCD1 and ATGL genes in the mammary gland and improve the milk quality of dairy goats by promoting the synthesis of short and medium-chain fatty acids in milk.

Cite this article

LI Jun, ZHAO Jinyan, HASI Tonglaga, LIU Taiyu, YANG Wenzhuo, WU Jiao, QUAN Kai . Effects of Fructus Corni Fruit Core on Lactation Performance, Milk Fatty Acid Composition and Expression of Lipid Metabolism Related Genes in Mammary Gland of Dairy Goats[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2303 -2312 . DOI: 10.3969/j.issn.1006-267x.2019.05.037

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