Effects of Ginseng Leaf Polysaccharide on Immune Performance of Yuehuang Chickens during 1 to 28 Days of Age

WEI Xiaochen, CHEN Ting, SUN Jiajie, LUO Junyi, NI Yuechun, SHU Gang, WANG Songbo, WANG Lina, JIANG Qingyan, ZHANG Yongliang, XI Qianyun

doi:10.3969/j.issn.1006-267x.2019.05.040

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 5: 2330 - 2339

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.040

Molecular Nutrition

Effects of Ginseng Leaf Polysaccharide on Immune Performance of Yuehuang Chickens during 1 to 28 Days of Age

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1. Guangdong Province Key Laboratory of Animal Nutritional Regulation, Guangzhou 510642, China;
2. National Engineering Research Center for Breeding Swine Industry, Guangzhou 510642, China;
3. College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2018-10-15

Online published: 2019-05-15

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Abstract

This experiment was conducted to study the effects of ginseng leaf polysaccharide on immune performance of Yuehuang chickens during 1 to 28 days of age. A total of 600 one-day-old Yuehuang chickens (hens) were randomly divided into 5 groups with 8 replicates per group and 15 chickens per replicate. Chickens in the group A (blank control group) were fed a basal diet, chickens in the group B (positive control group) were supplemented with 0.020% Astragalus polysaccharide, and others in groups C, D and E (ginseng leaf polysaccharide groups) were supplemented with 0.005%, 0.010%, 0.020% ginseng leaf polysaccharide, respectively. Polysaccharides were was added into drinking water. The experiment lasted for 28 days. The results showed as follows: 1) on 14 days of age, the thymus index of group E was significantly higher than that of group A (P<0.05). On 14 days of age, the spleen index of group D was significantly higher than that of group A (P<0.05); on 28 days of age, the spleen index of group B was significantly higher than that of group A (P<0.05). On 14 days of age, the bursa of Fabricius index of group E was significantly higher than that of other groups (P<0.05); on 28 days of age, the bursa of Fabricius index of group B was significantly higher than that of group A (P<0.05). 2) There were no significant differences in total protein, albumin, globulin creatinine, urea nitrogen contents and aminotransferase, aspartate aminotransferase activities in serum between ginseng leaf polysaccharide groups and group A (P>0.05). 3) On 21 days of age, the serum interleukin-2 (IL-2) content of groups B, C, D and E was significantly higher than that of group A (P<0.05); on 28 days of age, the serum IL-2 content of groups D and E was significantly higher than that of group A (P<0.05). On 14 days of age, the serum interleukin-12 (IL-12) content of groups C, D and E was significantly higher than that of group A (P<0.05); on 21 days of age, the serum IL-12 content of group A was significantly higher than that of other groups (P<0.05); on 28 days of age, the serum IL-12 content of group E was significantly lower than that of group A (P<0.05). On 14 days of age, the serum tumor necrosis factor-α (TNF-α) content of groups C and E was significantly higher than that of group A (P<0.05); on 21 days of age, the serum TNF-α content of group D was significantly higher than that of group A (P<0.05); on 28 days of age, the serum TNF-α content of groups B, C, D and E was significantly higher than that of group A (P<0.05). On 14 days of age, the serum γ-interferon (IFN-γ) content of groups C, D and E was significantly higher than that of groups A and B (P<0.05); on 21 days of age, the serum IFN-γ content of group C was significantly lower than that of other groups (P<0.05); on 28 days of age, the serum IFN-γ content of groups D and E was significantly higher than that of other groups (P<0.05). On 14 days of age, the serum natural killer (NK) cell content of group D was significantly higher than that of other groups (P<0.05); on 28 days of age, the serum NK cell content of group E was significantly higher than that of other groups (P<0.05). In summary, ginseng leaf polysaccharide can enhance the immune performance of Yuehuang chickens by increasing their immune organ index and the contents of IL-2, IL-12, TNF-α, IFN-γ and NK cells in serum.

Key words： ginseng leaf polysaccharides; Yuehuang chickens; immune performance; organ index; cytokine

Cite this article

WEI Xiaochen, CHEN Ting, SUN Jiajie, LUO Junyi, NI Yuechun, SHU Gang, WANG Songbo, WANG Lina, JIANG Qingyan, ZHANG Yongliang, XI Qianyun . Effects of Ginseng Leaf Polysaccharide on Immune Performance of Yuehuang Chickens during 1 to 28 Days of Age[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2330 -2339 . DOI: 10.3969/j.issn.1006-267x.2019.05.040

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