Molecular Nutrition

Effects of Different Functional Oligosaccharides on Bacteria Proliferation and Adhesion

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  • College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2018-11-26

  Online published: 2019-05-15

Abstract

This experiment was conducted to study the effects of different oligosaccharides on the proliferation of Lactobacillus, Escherichia coli and Salmonella, and the influence of Escherichia coli and Salmonella on the adhesion of pig jejunal epithelial cells (IPEC-J2).The effects of 8 functional oligosaccharides on the proliferation of Lactobacillus casei, Lactobacillus plantarum, Escherichia coli and Salmonella were determined with 0.25% addition. The effects of different functional oligosaccharides on the adhesion of Escherichia coli and Salmonella to IPEC-J2 were studied by using IPEC-J2 as an in vitro model and colony counting method. The results showed as follows: 1) there were significant differences in the proliferation of bacteria between the 8 functional oligosaccharides. Among them, fructo-oligosaccharides had the best proliferative effect on the two beneficial bacteria, while it had the lowest proliferative effect on pathogenic bacteria and had a trend to inhibit the growth. 2) Eight functional oligosaccharides could significantly reduce the adhesion of Escherichia coli and Salmonella to IPEC-J2 (P<0.05). Among them, manna oligosaccharides had the best inhibitory effect on the adhesion of Salmonella, while fructo-oligosaccharides had the most obvious effect on Escherichia coli. It is concluded that, in the experiments, functional oligosaccharides can not only promote the proliferation of beneficial bacteria, but also harmful bacteria, and the effects of different functional oligosaccharides on bacteria are significantly different; in addition, 8 functional oligosaccharides can significantly inhibit the adhesion of Escherichia coli and Salmonella to intestinal epithelial cells.

Cite this article

DU Chenhong, YANG Dongji, ZHU Suiliang, LIU Qian, ZHANG Jingjing, ZHANG Xiaotu, WANG Zhixiang . Effects of Different Functional Oligosaccharides on Bacteria Proliferation and Adhesion[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2378 -2387 . DOI: 10.3969/j.issn.1006-267x.2019.05.045

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