Effects of Zearalenone on Uterine Apoptosis and Apoptosis Factors of Mice in Different Periods

WANG Binglei, WANG Fei, ZHOU Lingqiang, CHEN Baojiang, CUI Yali, CUI Jia

doi:10.3969/j.issn.1006-267x.2019.05.046

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 5: 2388 - 2396

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.046

Molecular Nutrition

Effects of Zearalenone on Uterine Apoptosis and Apoptosis Factors of Mice in Different Periods

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1. College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China;
2. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China

Received date: 2018-10-22

Online published: 2019-05-15

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Abstract

The purpose of this study was to investigate the effects of different levels of zearalenone (ZEA) on the apoptosis of uterine cells and related factors in female mice in pre-estrus, estrus and post-partition periods. One hundred and twenty, 4-week-old, female Kunming mice were randomly divided into 4 groups, 30 in each group. Twenty male mice were randomly divided into 4 groups, 5 in each group. After 7 days of pre-trial period, the experiment was divided into two stages. Stage 1: the mice of the four groups were fed the basal diets with 0, 50, 100 and 150 mg/kg ZEA for 20 days. At days 2, 3 and 4 after the feeding test, ten mice in pre-estrus and estrus periods in each group were selected with vaginal smear. The selected mice were sacrificed by cervical delocation, and the uterine horns were harvested for TUNEL staining of apoptotic uterine cells and detection of apoptotic related factors mRNA expression. The remaining females of each group were caged with male mice by 2:1. After giving birth, the samples were collected according to the above method. The results showed as follows: 1) apoptotic cells in pre-estrus period were mainly located in the endometrial stroma and epithelial cells. Compared with the control group, adding 100, 150 mg/kg ZEA significantly increased the optical density of apoptotic cells (P<0.01), and significantly increased mRNA relative expression levels of the caspase-3, caspase-8, caspase-9 and B-cell lymphoma/leukemia-2 (Bcl-2) (P<0.01). 2) In estrous period, apoptosis was observed mainly in endometrial stromal cells, endometrial epithelial cells and uterine gland cells; compared with the control group, with the increasing of ZEA level, apoptotic cells significantly increased (P<0.01), and the mRNA relative expression levels of apoptotic genes significantly increased (P<0.01). 3) The distribution of apoptotic cells were more uniform in the post-partition period; compared with the control group, adding 150 mg/kg ZEA significantly increased apoptosis (P<0.01). Meanwhile, adding 100 mg/kg ZEA significantly up-regulated the mRNA relative expression levels of Bcl-2 and caspase-3 (P<0.05), and adding 150 mg/kg ZEA significantly up-regulated the mRNA relative expression levels of caspase-8 and caspase-9 (P<0.05). In conclusion, ZEA promots the apoptosis of mice uterus in different periods and up-regulats the expression of apoptosis-related genes caspase-3, caspase-8, caspase-9 and Bcl-2.

Key words： zearalenone; apoptosis; caspase-3; caspase-8; caspase-9; Bcl-2

Cite this article

WANG Binglei, WANG Fei, ZHOU Lingqiang, CHEN Baojiang, CUI Yali, CUI Jia . Effects of Zearalenone on Uterine Apoptosis and Apoptosis Factors of Mice in Different Periods[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2388 -2396 . DOI: 10.3969/j.issn.1006-267x.2019.05.046

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