Effects of Dietary Sea Buckthorn Pomace on Rumen Fermentation Parameters in Vitro and Effective Degradability of Nutrients

DIAO Xiaogao, HAO Xiaoyan, ZHAO Junxing, DING Na, YU Shengchen, XIANG Binwei, ZHANG Wenjia, ZAHNG Jianxin

doi:10.3969/j.issn.1006-267x.2019.05.050

2019 , Vol. 31 >Issue 5: 2423 - 2430

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.05.050

Molecular Nutrition

Effects of Dietary Sea Buckthorn Pomace on Rumen Fermentation Parameters in Vitro and Effective Degradability of Nutrients

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1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China;
2. Animal Husbandry Bureau of Youyu County, Youyu 037200, China

Received date: 2018-11-21

Online published: 2019-05-15

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Abstract

The purpose of this experiment was to investigate the effects of dietary sea buckthorn pomace (SBP) on rumen fermentation parameters and effective degradability of nutrients in vitro. Six healthy, 1.5-year-old, weight of 30 to 45 kg Dorper×thin-tailed Han crossbred Capricorn meat sheep F₁ with installed rumen fistulas were selected. In vitro gas production method and nylon bag method were used to determine the 72 h gas production, rumen fluid pH, ammoniacal nitrogen (NH₃-N), volatile fatty acids (VFA), microbe protein (MCP) concentrations and the effective degradability of nutrients of total mixed ration with SBP at different supplemental levels of 0 (control group), 8% (8SBP group), 16% (16SBP group) and 24% (24SBP group), respectively. The results showed as follows: 1) 72 h gas production and potential gas production in 16SBP and 24SBP groups were significantly higher than those in the control group (P<0.05), but adding SBP had no significant effect on the gas production rate (P>0.05). 2) The pH decreased with the increase of SBP supplement level, and the 24SBP group was the lowest (P<0.05). Compared with the control group, the NH₃-N and MCP concentrations in experimental groups had an increased trend, but the difference was not significant (P>0.05); methane (CH₄) and CO₂ production were affected, but there were no significant differences among groups (P>0.05). The concentrations of acetic acid and total VFA in experimental groups were significanlty higher than those in the control group (P<0.05). 3) Compared with the control group, the effective degradability of dry matter and acid detergent fiber in 16SBP and 24SBP groups significantly increased (P<0.05), and the effective degradability of crude protein in 8SBP and 16SBP groups significantly increased (P<0.05). It is concluded that the addition of SBP to the diet can improve in vitro fermentation and the degradation rate of nutrients, but the optimal supplemental level of SBP in the diet needs to be further determined by feeding experiments.

Key words： sea buckthorn pomace; in vitro gas production method; nylon bag method; gas production; effective degradability

Cite this article

DIAO Xiaogao, HAO Xiaoyan, ZHAO Junxing, DING Na, YU Shengchen, XIANG Binwei, ZHANG Wenjia, ZAHNG Jianxin . Effects of Dietary Sea Buckthorn Pomace on Rumen Fermentation Parameters in Vitro and Effective Degradability of Nutrients[J]. Chinese Journal of Animal Nutrition, 2019 , 31(5) : 2423 -2430 . DOI: 10.3969/j.issn.1006-267x.2019.05.050

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