Effects of Dietary Fermented Soybean Meal on Diversity of Faecal Microbiota of Weaned Pigs

HE Min, DANG Wenqing, LIANG Xionglong, YANG Jinqing, ZHANG Jie, LIANG Jiyu, YU Muhang

doi:10.3969/j.issn.1006-267x.2019.06.015

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 6: 2560 - 2571

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.06.015

Molecular Nutrition

Effects of Dietary Fermented Soybean Meal on Diversity of Faecal Microbiota of Weaned Pigs

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Institute of Animal Husbandry & Veterinary, Sciences of Shanxi Agricultural Academy, Taiyuan 030032, China

Received date: 2018-10-15

Online published: 2019-06-17

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Abstract

This study was conducted to study the effects of dietary fermented soybean meal (FSM) on the fecal microbiota diversity of weaned piglets using Illumina high-throughput sequencing technology. A total of 80 piglets were randomly divided into A, B, C and D groups. As the control, the piglets of group D were fed a basal diet. The piglets of groups A,B and C were fed the basal diet added 5%, 10% and 15% FSM, respectively. Feces of four groups were collected after the experiment. The V3 to V4 region of the 16S rRNA gene of bacteria in faeces was sequenced using Illumina high-throughput sequencing technology. The results showed as follows:1) in all of the samples, the average effective sequence number of bacteria was 47 218 and 3 581 operational taxonomic unit (OTUs) were produced at a similar level of 97% and 12 phylum, 21 classes, 33 orders, 64 families, 111 genera and 137 species were detected. 2) The alpha diversity indexes of fecal microbiota showed that there was no significant difference among groups (P>0.05), but the Shannon index of group B was the highest, indicating that the diversity of this group was the best. The beta analysis of diversity showed that the grouping effect was better, and there were significant differences between groups (P<0.05). 3) At the phylum level, the dominant bacteria in feces was Firmicutes. The relative abundance of Firmicutes of group B was significantly higher than that of group D (P<0.05). Both the family and genus levels, the dominant bacteria was Lactobacillus and Lactobacillus, respectively. The relative abundance of Lactobacillus of group B was significantly higher than that of groups A and D (P<0.05), but no significant differences between group B and group C (P>0.05). 4) It was predicted that the bacteria were mainly involved in metabolic pathways of carbohydrate, amino acid, energy, nucleic acid, and cofactors and vitamins. In addition to the nucleic acid metabolism, the relative abundance of bacteria of group B was the greatest and followed by groups C, A and D. In conclusion, adding 10% FSM to the piglet diets can increase the diversity of the intestinal bacteria, significantly increase the relative abundance of beneficial bacteria such as Firmicutes, Lactobacillus and Lactobacillus, maintain the healthy development and promote the metabolism of nutrients.

Key words： fermented soybean meal; weaned piglets; faeces; microbiota diversity; Illumina MiSeq sequencing

Cite this article

HE Min, DANG Wenqing, LIANG Xionglong, YANG Jinqing, ZHANG Jie, LIANG Jiyu, YU Muhang . Effects of Dietary Fermented Soybean Meal on Diversity of Faecal Microbiota of Weaned Pigs[J]. Chinese Journal of Animal Nutrition, 2019 , 31(6) : 2560 -2571 . DOI: 10.3969/j.issn.1006-267x.2019.06.015

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