Effects of Attapulgite Nano Zinc Oxide on Liver Immune Function and Lipid Metabolism of Weaned Piglets

ZHONG Zhaoxin, DONG Li, MAO Junzhou, ZHANG Yongsheng, WANG Tianlong, YU Lihuai

doi:10.3969/j.issn.1006-267x.2019.06.020

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 6: 2605 - 2613

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.06.020

Molecular Nutrition

Effects of Attapulgite Nano Zinc Oxide on Liver Immune Function and Lipid Metabolism of Weaned Piglets

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2018-12-11

Online published: 2019-06-17

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Abstract

The experiment was conducted to explore the effects of attapulgite nano zinc oxide on liver immune function and lipid metabolism of weaned piglets. A total of 210 21-day-old piglets with similar body weight of (6.30±0.51) kg were randomly allocated into 7 groups with 6 replicates each. The groups were control group (CON group, fed a basal diet), antibiotic group (ANT group, fed the basal diet supplemented with 100 mg/kg 50% olaquindox ethanol+150 mg/kg 15% aureomycin +50 mg/kg 10% colistin sulfate), zinc oxide group (ZO group, fed the basal diet supplemented with 3 000 mg/kg zinc oxide), nano zinc oxide group (NZO group, fed the basal diet supplemented with 800 mg/kg nano zinc oxide), low attapulgite nano zinc oxide group (LA-ZO group, fed the basal diet supplemented with 700 mg/kg attapulgite nano zinc oxide), medium attapulgite nano zinc oxide group (MA-ZO group, fed the basal diet supplemented with 1 000 mg/kg attapulgite nano zinc oxide), and high attapulgite nano zinc oxide group (HA-ZO group, fed the basal diet supplemented with 1 300 mg/kg attapulgite nano zinc oxide, respectively. The results showed as follows:1) the tumor necrosis factor-α (TNF-α) content in liver of piglets in LA-ZO group was significantly lower than those in ZO, MA-ZO and CON groups (P<0.05). Compared with CON and NZO groups, interleukin-4 content in liver of piglets in LA-ZO and MA-ZO groups was significantly increased (P<0.05). The cytokine content in liver of piglets in HA-ZO was not significantly different from that in CON group (P>0.05). 2) The expression of TNF-α in liver of piglets in NZO and LA-ZO groups was significantly lower than that in CON group (P<0.05). There was no significant difference in the expression of cytokine-related genes in liver of piglets in HA-ZO group compared with CON group (P>0.05). 3) The low density lipoprotein cholesterin content in liver of piglets in LA-ZO and MA-ZO groups was significantly lower than those in CON and ANT groups (P<0.05). The total cholesterol content in liver of piglets in LA-ZO and NZO groups were significantly decreased compared with CON, ANT and ZO groups (P<0.05). The lipid metabolism-related product contents in liver of piglets in HA-ZO group were not significantly different from those in CON group (P>0.05). 4) The fatty acid synthetase mRNA expression in liver of piglets in LA-ZO, ANT and MA-ZO groups was significantly lower than that in CON, ZO and NZO groups (P<0.05); the hormone-sensitive lipase mRNA expression in liver of piglets in LA-ZO group was significantly higher than that in CON, ANT and ZO groups (P<0.05). There was no significant difference in the expression of lipid metabolism related genes in liver of piglets in HA-ZO group compared with CON group (P>0.05). In summary, compared with CON group, the supplementation of attapulgite nano zinc oxide can improve the liver immune function and lipid metabolism of weaned piglets in some extent. This experiment results show that the addition of 700 mg/kg attapulgite nano zinc oxide is suitable.

Key words： weaned piglets; attapulgite nano zinc oxide; liver; immune function; lipid metabolism

Cite this article

ZHONG Zhaoxin, DONG Li, MAO Junzhou, ZHANG Yongsheng, WANG Tianlong, YU Lihuai . Effects of Attapulgite Nano Zinc Oxide on Liver Immune Function and Lipid Metabolism of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2019 , 31(6) : 2605 -2613 . DOI: 10.3969/j.issn.1006-267x.2019.06.020

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