Molecular Nutrition

Regulation of γ-Aminobutyric Acid on Hypothalamic-Pituitary-Adrenal Axis of Heat-Stressed Chickens In Summer

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  • 1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
    2. Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei 230031, China

Received date: 2018-11-14

  Online published: 2019-06-17

Abstract

The aim of this study was to investigate the regulation of γ-aminobutyric acid (GABA) on the hypothalamic-pituitary-adrenal (HPA) axis of heat-stressed chickens in summer and its mechanism. One hundred and twenty 28-day-old Huainan partridge female chickens were randomly divided into 4 groups with 5 replicates per group and 6 chickens per replicate. Chickens in group Ⅰ (control group) were fed a basal diet, and others in groups Ⅱ, Ⅲ and Ⅳ were fed the basal diets supplemented with 50, 100 and 150 mg/kg GABA, respectively. The pre-experimental period lasted for 7 days, and the experimental period lasted for 30 days. The temperature and relative humidity of the house and the feed consumption of chickens in each group were recorded daily. On day 10, 20 and 30, five chickens were randomly selected from each group, after weighing, serum was collected to detect the contents of corticotropin-releasing hormone (CRH), adreno-cortico-tropic-hormone (ACTH), corticotropin (Cor), heat shock protein 70 (HSP70) and GABA; the brain was taken from chickens of groups Ⅰ and Ⅲ to detect the content of GABA in brain tissue. The results showed as follows:1) on day 10, 20 and 30, the rectal temperature of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05 or P<0.01). 2) During day 21 to 30, the average daily feed intake of groups Ⅱ and Ⅲ was significantly higher than that of group Ⅰ (P<0.05). During day 1 to 10, day 11 to 20 and day 21 to 30, the average daily gain of groups Ⅲ and Ⅳ was significantly higher than that of group Ⅰ (P<0.05 or P<0.01). 3) On day 10, the serum CRH content of groups Ⅱ and Ⅲ was significantly lower than that of group Ⅰ (P<0.05 or P<0.01); on day 30, the serum CRH content of groups Ⅲ and Ⅳ was significantly lower than that of group Ⅰ (P<0.05). On day 10 and 30, the serum ACTH content of groups Ⅱ and Ⅲ was significantly lower than that of group Ⅰ (P<0.05 or P<0.01); on day 20, the serum ACTH content of group Ⅲ was significantly lower than that of group Ⅰ (P<0.05). On day 10, the serum Cor content of group Ⅲ was significantly lower than that of group Ⅰ (P<0.05); on day 30, the serum Cor content of groups Ⅱ, Ⅲ and Ⅳ was significantly lower than that of group Ⅰ (P<0.01). 4) On day 10 and 30, the serum HSP70 content of groups Ⅱ and Ⅲ was significantly lower than that of group Ⅰ (P<0.05 or P<0.01); on day 20, the serum HSP70 content of group Ⅲ was significantly lower than that of group Ⅰ (P<0.01). 5) On day 10, the serum GABA content of groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that of group Ⅰ (P<0.05 or P<0.01); on day 20 and 30, the serum GABA content of group Ⅳ was significantly higher than that of group Ⅰ (P<0.01). 6) On day 10 and 30, the brain tissue GABA content of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05). In conclusion, dietary GABA reduce the rectal temperature and contents of CRH, ACTH, Cor and HSP70 in serum of chickens, and group Ⅲ has the best effect. GABA has the function of inhibiting the secretion of HPA axis hormones of heat-stressed chickens in summer.

Cite this article

QIAN Shoufa, JIANG Dan, MA Yuying, LI Xinlu, WEI Jing, CHEN Jinxia, YANG Xiaoxiao, WU Jinjie, ZHAN Kai, LI Jinchun . Regulation of γ-Aminobutyric Acid on Hypothalamic-Pituitary-Adrenal Axis of Heat-Stressed Chickens In Summer[J]. Chinese Journal of Animal Nutrition, 2019 , 31(6) : 2625 -2633 . DOI: 10.3969/j.issn.1006-267x.2019.06.022

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