Effects of Dietary Vitamin K3 Supplemental Level on Tibia Development, Immune Organ Index and Antioxidant Capacity of Wulong Geese

XU Yanhong, YIN Taiyue, WANG Baowei, WANG Xiaowei, LI Wenli

doi:10.3969/j.issn.1006-267x.2019.06.024

2019 , Vol. 31 >Issue 6: 2642 - 2650

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.06.024

Molecular Nutrition

Effects of Dietary Vitamin K₃ Supplemental Level on Tibia Development, Immune Organ Index and Antioxidant Capacity of Wulong Geese

Expand

1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
2. Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China;
3. Laiyang Bureau of Animal Husbandry in Shandong Province, Laiyang 265200, China

Received date: 2018-11-21

Online published: 2019-06-17

Fold

Abstract

This experiment was conducted to study the effects of dietary vitamin K₃ supplemental level on tibia development, immune organ index and antioxidant capacity of Wulong geese. This experiment was divided into two stages, in 1 to 4 weeks of age stage, a total of 360 one-day-old Wulong geese were selected and randomly divided into 6 groups with 6 replicates in each group and 10 geese in each replicate. Geese in group Ⅰ (control group) were fed a basal diet (vitamin K₃ content was 1.23 mg/kg); and the others in groups Ⅱ to Ⅵ were fed the basal diets supplemented with 1, 2, 4, 8 and 16 mg/kg vitamin K₃, respectively. In 5 to 16 weeks of age stage, a total of 288 twenty-eight-day-old Wulong geese were selected and randomly divided into 6 groups with 6 replicates in each group and 8 geese in each replicate. Geese in group Ⅰ (control group) were fed a basal diet (vitamin K₃ content was 1.18 mg/kg); and the others in groups Ⅱ to Ⅵ were fed the basal diets supplemented with 2, 4, 8, 16 and 32 mg/kg vitamin K₃, respectively. The experiment lasted for 16 weeks. The results showed as follows:1) compared with the control group, in 1 to 4 weeks of age stage, dietary supplemented with 4 mg/kg vitamin K₃ significantly increased the crude ash content, bone mineral density, bone strength and bone weight in tibia (P<0.05), and significantly increased the contents of calcium and phosphorus in tibia (P<0.01); in 5 to 16 weeks of age stage, dietary supplemented with 8 mg/kg vitamin K₃ significantly increased the crude ash, calcium and phosphorus contents and bone mineral density, bone strength in tibia (P<0.01), and the bone trabecula and osteoblast developed better. 2) Dietary supplemented with vitamin K₃ had no significant effects on the thymus index, spleen index and bursa of Fabricius index (P>0.05). 3) Compared with the control group, in 1 to 4 weeks of age stage, dietary supplemented with 4 mg/kg vitamin K₃ significantly increased the serum total superoxide dismutase (T-SOD) activity, significantly decreased the serum malondialdehyde (MDA) content (P<0.05), and significantly increased the serum total antioxidant capacity (T-AOC) (P<0.01); in 5 to 16 weeks of age stage, dietary supplemented with 8 mg/kg vitamin K₃ significantly increased the serum T-SOD and catalase (CAT) activities, significantly decreased the serum MDA content (P<0.05), and significantly increased the serum T-AOC (P<0.01). In conclusion, the supplementation of 4 and 8 mg/kg vitamin K₃ in the diet of Wulong geese during 1 to 4 weeks of age and 5 to 16 weeks of age can increase the crude ash, calcium and phosphorus contents and bone mineral density, bone strength of tibia, and improve the development of tibia. It also can increase the T-SOD activity and T-AOC in serum, reduce the serum MDA content, and improve the antioxidant capacity of Wulong geese.

Key words： vitamin K₃; Wulong geese; tibia development; immune organ index; antioxidant capacity

Cite this article

XU Yanhong, YIN Taiyue, WANG Baowei, WANG Xiaowei, LI Wenli . Effects of Dietary Vitamin K₃ Supplemental Level on Tibia Development, Immune Organ Index and Antioxidant Capacity of Wulong Geese[J]. Chinese Journal of Animal Nutrition, 2019 , 31(6) : 2642 -2650 . DOI: 10.3969/j.issn.1006-267x.2019.06.024

References

[1] 党晓鹏.畜禽维生素K的非凝血功能[J].江西饲料, 2015, 6(3):6-8.

[2] YANG T T C, KOO M W L.Inhibitory effect of Chinese green tea on endothelial cell-induced LDL oxidation[J].Atherosclerosis, 2000, 148(1):67-73.

[3] 陶天遵, 杨小清, 陶树清, 等.维生素K与骨代谢[J].国外医学(内分泌学分册), 2005, 25(5):298-301.

[4] KIM M, NA W, SOHN C.Vitamin K₁ (phylloquinone) and K₂ (menaquinone-4) supplementation improves bone formation in a high-fat diet-induced obese mice[J].Journal of Clinical Biochemistry and Nutrition, 2013, 53(2):108-113.

[5] BOUCKAERT J H, SAID A H.Fracture healing by vitamin K[J].Nature, 1960, 18(5):849-850.

[6] FANG YZ, YANG S, WU G Y.Freeradicals, antioxidants, and nutrition[J].Nutrition, 2002, 18(10):872-879.

[7] YUAN J, FENG L, JIANG W D, et al.Effects of dietary vitamin K levels on growth performance, enzyme activities and antioxidant status in the hepatopancreas and intestine of juvenile Jian carp (Cyprinuscarpio var.Jian)[J].Aquaculture Nutrition, 2016, 22(2):352-366.

[8] FU J H, XU W, MAI K S, et al.Effects of dietary menadione on the activity of antioxidant enzymes in abalone, Haliotis discus hannaniino[J].Chinese Journal of Oceanology and Limnology, 2012, 30(1):118-123.

[9] SOPER R J, OGUZ C, EMERY R, et al.Vitamin K catabolite inhibition of ovariectomy-induced bone loss:structure-activity relationship considerations[J].Molecular Nutrition &Food Research, 2014, 58(8):1658-1666.

[10] 谢煜, 姚珍薇, 杨帆.雌激素和维生素K抗去势成年雌性大鼠骨质疏松的实验研究[J].中华妇幼临床医学杂志(电子版), 2009, 5(3):226-231.

[11] YAMAUCHI M, YAMAGUCHI T, NAWATA K, et al.Relationships between undercarboxylated osteocalcin and vitamin K intakes, boneturnover, and bone mineral density in healthy women[J].Clinical Nutrition, 2010, 29(6):761-765.

[12] 罗林枝, 徐苓.维生素K与骨质疏松[J].中国医学科学院学报, 2003, 25(3):346-349.

[13] 赵新华, 赵熙和, 崔伟, 等.维生素K对幼鼠骨量及骨强度影响的实验研究[J].中国骨质疏松杂志, 1999, 5(1):22-27.

[14] 高志.维生素K₂对去卵巢大鼠骨质疏松症的影响[D].硕士学位论文.成都:四川大学, 2007.

[15] COCKAYNE S, ADAMSON J, LANHAM-NEWS, et al.Vitamin K and the prevention of fractures:systematic review and meta-analysis of randomized controlled trials[J].Archives of Internal Medicine, 2006, 166(12):1256-1261.

[16] SHIRAKI M, SHIRAKI Y, AOKI C, et al.Vitamin K₂ (menatetrenone) effectively prevents fractures and sustains lumbar bone mineral density in osteoporosis[J].Journal of Bone and Mineral Research, 2000, 15(3):515-521.

[17] 元江.维生素K对幼建鲤消化吸收功能、免疫功能和抗氧化状态的影响[D].硕士学位论文.雅安:四川农业大学, 2013.

[18] 郭丹苗.人血免疫球蛋白联合维生素K₃治疗难治性哮喘的临床疗效研究[J].中外医学研究, 2013, 11(22):33-34.

[19] 常连胜, 冯陶, 李菊香, 等.维生素D₃和维生素K₃对实验性肾结石的影响[J].中华泌尿外科杂志, 2001, 22(1):20-22.

[20] 吴亚锋, 陈静, 倪金娣, 等.3种免疫增强剂对异育银鲫非特异性免疫功能的影响[J].水产养殖, 2018, 39(10):23-27.

[21] CANFIELD L M, DAVY L A, THOMAS G L.Anti-oxidant/pro-oxidant reactions of vitamin K[J].Biochemical and Biophysical Research Communications, 1985, 128(1):211-219.

[22] 潘春芳, 徐三荣.维生素K对去卵巢大鼠肝氧化应激及炎症因子的影响[J].江苏大学学报(医学版), 2014, 24(1):31-35.

[23] 李国富, 何一凡, 吴清坛, 等.血清SOD、GSH-Px、T-AOC及MDA的测定对肺炎诊断的临床意义[J].现代临床医学生物工程学杂志, 2003, 9(3):196-197, 200.

[24] 王连生, 许红, 王洋, 等.维生素K₃对西伯利亚鲟生长和抗氧化功能的影响[J].大连海洋大学学报, 2015, 30(6):641-646.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References