Effectiveness of Methionine Dipeptide in Juvenile Common Carp Diet and Methionine Requirement of Juvenile Common Carp Re-Evaluated by Methionine Dipeptide

YIN Ziyu, HE Juyun, LIANG Xiaofang, XUE Min, ZHENG Yinhua, GU Xu, WU Xiufeng

doi:10.3969/j.issn.1006-267x.2019.06.037

2019 , Vol. 31 >Issue 6: 2765 - 2776

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.06.037

Molecular Nutrition

Effectiveness of Methionine Dipeptide in Juvenile Common Carp Diet and Methionine Requirement of Juvenile Common Carp Re-Evaluated by Methionine Dipeptide

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1. National Aquafeed Safety Assessment Station, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Evonik Degussa(China) Co., Ltd., Branch Company in Guangzhou, Guangzhou 510050, China;
3. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2018-11-21

Online published: 2019-06-17

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Abstract

This experiment was aim to evaluate the effectiveness of methionine dipeptide (Met-Met) in juvenile common carp diet and to determine the methionine (Met) requirement of juvenile common carp (Cyprinus carpio) by adding different levels of Met-Met into the diet. Juvenile common carp with the body weight of (16.70±0.08) g randomly assigned to 7 groups with 6 replicates per group and 30 juvenile fish per replicate. Using pea concentrated protein as protein source, six experimental diets supplemented with 0, 1.5, 2.0, 2.5, 3.0 and 4.0 g/kg Met-Met were prepared based on a low Met diets with 4.8 g/kg Met and 4.4 g/kg cysteine (Cys) to fed fish in 6 groups, which was named as M0, M1.5, M2, M2.5, M3 and M4 groups, respectively. Fish in the last group were fed a diet contained 10% fish meal and supplemented with 3.0 g/kg crystal DL-Met, which was named as FM group (positive control group). The feeding trial lasted for 10 weeks. The results showed as follows:compared with M0 group, the final average body weight, weight gain rate (WGR) and specific growth rate (SGR) were significantly increased and the feed conversion ratio (FCR) was significantly decreased in each Met-Met group (P<0.05). Taking WGR and FCR as assessment indexes, the Met requirements of juvenile common carp were 8.1 and 7.9 g/kg according to monodic quadratic regression model analysis, respectively. Taking protein retention rate (PRR) as assessment index, the Met requirement of juvenile common carp was 7.6 g/kg according to broken-line regression analysis. Diets supplemented with different levels of Met-Met all could significantly increase the contents of Met, Met+Cys, lysine (Lys), tryptophan (Trp), isoleucine (Ile), leucine (Leu), valine (Val) and histidine (His) in whole body, but there were no significant differences between each Met-Met group and FM group (P>0.05). Essential amino-acid retention rate in M0 group was significantly lower than that in other groups (P<0.05). Met retention rate showed a decrease trend with the Met-Met supplemental level increasing, and the Met retention rate in M0, M1.5, M2 and M2.5 groups was significantly higher than that in FM group (P<0.05). Lys retention rate in M0 group was significantly lower than that in Met-Met groups except M1.5 group (P<0.05), and there was no significant difference between each Met-Met group and FM group (P>0.05). Plasma high density lipoprotein cholesterol (HDL-C)/total cholesterol (TC) in M4 group was significantly higher than that in FM and M0 groups (P<0.05). Plasma total antioxidant capacity (T-AOC) in M2 and M4 groups was significantly higher than that in M0 group (P<0.05). Plasma malondialdehyde (MDA) content in M3 group was the lowest, which was significantly lower than that in other groups except M1.5 and M4 groups (P<0.05). In conclusion, suitable level of Met-Met added into a Met deficiency diet with 4.8 g/kg Met can effectively promote the growth and feeding, improve the transport and metabolism of lipid, and increase antioxidant ability of juvenile common carp. Met requirement of juvenile common carp is estimated to be 7.6 to 8.1 g/kg (the supplemental level of Met-Met in the diet is 2.95 to 3.47 g/kg), which account for 2.0% to 2.1% dietary protein, by regression analysis base on WGR, FCR and PRR. The Met supplied by Met-Met has the similar characteristics in digestion, absorption and utilization with the Met from natural protein source. So, it is can be more accurate to evaluate the Met requirement of juvenile common carp using Met-Met.

Key words： juvenile common carp (Cyprinus carpio); Met-Met; Met requirement; growth performance; regression model; re-evaluation

Cite this article

YIN Ziyu, HE Juyun, LIANG Xiaofang, XUE Min, ZHENG Yinhua, GU Xu, WU Xiufeng . Effectiveness of Methionine Dipeptide in Juvenile Common Carp Diet and Methionine Requirement of Juvenile Common Carp Re-Evaluated by Methionine Dipeptide[J]. Chinese Journal of Animal Nutrition, 2019 , 31(6) : 2765 -2776 . DOI: 10.3969/j.issn.1006-267x.2019.06.037

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