Effects of Selenium Sources and Selenium Levels on Growth Performance, Immune Enzyme activities and Selenium Contents of Whole Fish and Vertebrae for Juvenile Grouper (Epinephelus coioide)

LIANG Dazhi, MA Haoyong, YANG Qihui, TAN Beiping, DONG Xiaohui, CHI Shuyan, LIU Hongyu, ZHANG Shuang, ZHANG Haitao

doi:10.3969/j.issn.1006-267x.2019.06.038

2019 , Vol. 31 >Issue 6: 2777 - 2787

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.06.038

Molecular Nutrition

Effects of Selenium Sources and Selenium Levels on Growth Performance, Immune Enzyme activities and Selenium Contents of Whole Fish and Vertebrae for Juvenile Grouper (Epinephelus coioide)

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1. Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University, Zhanjiang 524088, China;
2. Zhanjiang Aohua Aquatic Feed Co., Ltd., Zhanjiang 524047, China;
3. Accurate Nutrition and High Efficiency Feed Engineering Research Center for Aquatic Animals in Guangdong province, Zhanjiang 524074, China

Received date: 2018-10-22

Online published: 2019-06-17

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Abstract

This experiment was conducted to study the effects of selenium sources and selenium levels on growth performance, immune enzyme activities and selenium contents of whole fish and vertebrae for juvenile grouper (Epinephelus coioide), and to determine the optimal selenium requirement in diets and relative bioavailability. A total of 810 juvenile grouper with an initial average weight of (8.99±0.03) g were randomly assigned into 9 groups with 3 replicates per group.Selenite sodium (Se-S) and selenite methionine (Se-Met) were used as selenite sources, and five selenium supplemental levels were 0, 0.3, 0.6, 0.9 and 1.2 mg/kg, respectively. Selenium level in a basal diet was 0.48 mg/kg; selenium levels in diets of Se-S groups were 0.82, 1.15, 1.32 and 1.79 mg/kg; selenium levels in diets of Se-Met groups were 0.81, 1.18, 1.51 and 1.94 mg/kg, respectively. Raising period was 10 weeks. The results showed as follows:1) dietary selenium supplemental levels had significant effects on weight gain rate (WGR) and specific growth rate (SGR) of juvenile grouper (P<0.05), and had no significant effects on survival rate (SR) and feed conversion ratio (FCR) of juvenile grouper (P>0.05). When Se-S used as the selenium source, the diet supplemented with 1.15 mg/kg selenium had the highest WGR and SGR, and when Se-Met used as the selenium source, the diet supplemented with 1.18 mg/kg selenium had the highest WGR and SGR. 2) The different dietary selenium levels significantly affected the activities of serum alkaline phosphatase (AKP) and liver total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the content of liver malondialdehyde (MDA) (P<0.05). 3) The selenium contents in the whole fish and vertebrae were linear increased with the increased selenium levels, and vertebral selenium content was much lower than that of the whole fish. In conclusion, with the SGR as an evaluation index and the Se-S and Se-Met as the selenium sources, the optimal supplemental levels of selenium in juvenile grouper diets are 0.98 and 1.01 mg/kg, respectively. Under this experimental condition, the bioavailability of Se-Met is higher than that of Se-S, which is 112.5% of that of Se-S.

Key words： Epinephelus coioide; selenium; growth performance; immune enzyme activity; selenium content

Cite this article

LIANG Dazhi, MA Haoyong, YANG Qihui, TAN Beiping, DONG Xiaohui, CHI Shuyan, LIU Hongyu, ZHANG Shuang, ZHANG Haitao . Effects of Selenium Sources and Selenium Levels on Growth Performance, Immune Enzyme activities and Selenium Contents of Whole Fish and Vertebrae for Juvenile Grouper (Epinephelus coioide)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(6) : 2777 -2787 . DOI: 10.3969/j.issn.1006-267x.2019.06.038

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