Molecular Nutrition

Determination of Crude Fiber Content in Feeds Using Polyester Mesh Bag Method

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  • Key Laboratory of Animal Biotechnology and Disease Control & Prevention of Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2018-11-23

  Online published: 2019-06-17

Abstract

This study aimed to explore a rapid method of using polyester mesh bag to determine crude fiber (CF) content in feeds, and to evaluate the accuracy and precision of this method. The CF contents in 18 kinds of feeds such as soybean meal, wheat bran, rice bran, alfalfa and peanut vine and so on were analyzed using the polyester mesh bag method, traditional method(GB/T 6434-2006) and Ankom bag fiber determinator method. The CF contents in 8 kinds of feeds such as wheat bran, rice bran, peanut vine, swine compound feed, rabbit compound feed, alfalfa, peanut vine and rice hull were analyzed in 10 different laboratories using the polyester mesh bag method for assessing repeatability limit and reproducibility limit of this method. The results showed that there were no significant difference between the polyester mesh bag method and traditional method (P>0.05), indicating that the polyester mesh bag method had higher accuracy. The repeatability variability coefficients of 8 kinds of feeds used in this study, ranged from 1.45% to 3.64%, and the reproducibility variation coefficient was between 1.79% and 5.64%. The repeatability limit was between 0.57% and 1.99%, and the reproducibility limit was between 0.88% and 3.47%, indicating that the polyester mesh bag method had higher precision. In conclusion, the polyester mesh bag method for determining CF contents has the advantages of lower cost, shorter analysis time, higher efficiency and higher accuracy and precision; therefore, this method can determine CF contents of large quantities of samples.

Cite this article

ZHANG Chen, LI Zemin, ZHANG Jie, ZHANG Guiguo, ZHANG Chongyu . Determination of Crude Fiber Content in Feeds Using Polyester Mesh Bag Method[J]. Chinese Journal of Animal Nutrition, 2019 , 31(6) : 2875 -2881 . DOI: 10.3969/j.issn.1006-267x.2019.06.048

References

[1] 刘磊, 李福昌, 杨鹏程, 等.饲料粗纤维、中性洗涤纤维和酸性洗涤纤维残渣中各成分的研究[J].动物营养学报, 2018, 30(3):1044-1051.

[2] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 6434-2006饲料中粗纤维的含量测定过滤法[S].北京:中国标准出版社, 2006.

[3] VOGEL K P, PEDERSEN J F, MASTERSON S D, et al.Evaluation of a filter bag system for NDF, ADF, and IVDMD forage analysis[J].Crop Science, 1999, 39(1):276-279.  

[4] KOMAREK A R.A filter bag procedure for improved efficiency of fiber analysis[J].Journal of Dairy Science, 1993, 76(Suppl.1):250-254.

[5] KOMARK A R, ROBERTSON J B, VAN SOEST P J.A comparison of techniques for techniques for determining ADF using the filter bag technique versus conventional filtration[J].Journal of Dairy Science, 1994, 77(Suppl.1):114-123.

[6] 王晓娜, 徐春城, 温定英, 等.不同测定方法对青贮饲料中NDF和ADF含量的影响[J].草业科学, 2012, 29(1):144-149.

[7] 薛红枫, 孟庆翔.不同方法测定反刍动物饲料NDF、ADF和木质素含量的比较[J].中国畜牧杂志, 2006, 42(19):41-45.

[8] 闫贵龙, 程成, 曹春梅, 等.煮沸时间对滤袋法测定青贮玉米NDF和ADF含量的影响[J].畜牧兽医学报, 2012, 43(3):404-409.

[9] 朱立涛, 范志勇, 杨丹丹, 等.用滤袋测定饲料粗纤维含量的试验研究[J].饲料工业, 2012, 33(7):56.

[10] 张森, 张媛媛, 苏宇辰, 等.饲料粒度及分布对国标法和滤袋法测定粗纤维的影响及两种方法的差异比较[J].粮食与饲料工业, 2015(9):62-66.

[11] 魏涛, 蒲俊华, 王文强, 等.Ankom滤袋法测定粗纤维含量过程中样品脱脂与不脱脂处理的比较[J].黑龙江畜牧兽医, 2017(8):275-277.

[12] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 14014-2008合成纤维筛网[S].北京:中国标准出版社, 2009.

[13] 张崇玉, 张桂国, 高志标, 等.聚酯筛网袋法测定饲料中CF和NDF含量的精密度研究[J].山东畜牧兽医, 2016, 37(8):1-3.

[14] 张崇玉, 王保哲, 张桂国, 等.饲料中的粗纤维、NDF、ADF和ADL含量的快速测定方法[J].山东畜牧兽医, 2015, 36(9):20-22.

[15] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 6379.2-2004测量方法与结果的准确度(正确度与精密度)第2部分确定标准测量方法重复性与再现性的基本方法[S].北京:中国标准出版社, 2005.

[16] 田雨佳, 曹志军, 李胜利, 等.不同粉碎粒度的饲料对滤袋法测定纤维物质含量的影响[J].动物营养学报, 2013, 25(2):350-355.

[17] 姜慧新, 柏杉杉, 刘栋, 等.不同方法测定饲草中NDF与ADF含量的比较[J].山东畜牧兽医, 2017, 38(8):6-7.

[18] 刘胜男, 孙燕.滤袋法测定饲料粗纤维的操作流程及注意要点[J].饲料与畜牧, 2017(15):55-56.

[19] 王仁华, 刘小兰, 敖小兰, 等.样品粒度对粗纤维测定值的影晌[J].江西畜牧兽医杂志, 2000(5):36.

[20] 张桂英.改进饲料中粗纤维的测定方法[J].民营科技, 2018(5):10.

[21] 魏时来, 蔺淑琴, 李典芬, 等.饲粮样品粉碎粒度对ADF和NDF尼龙滤袋法测定值的影响[J].畜牧兽医杂志, 2008, 27(4):3-5.

[22] International Organization for Standardization.ISO 6865:2000 animal feeding stuffs-determination of crude fibre content-method with intermediate filtration[S].Switzerland:International Organization for Standardization, 2000.
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