Effects of Meal Frequency on Growth Performance and Muscle Protein Synthesis in Suckling Piglets

LIU Jingbo, CAO Shanchuan, YANG Yong, ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2019.07.015

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 7: 3049 - 3057

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.015

Molecular Nutrition

Effects of Meal Frequency on Growth Performance and Muscle Protein Synthesis in Suckling Piglets

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1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2019-01-02

Online published: 2019-07-19

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Abstract

The present study was conducted to determine the effects of meal frequency on growth performance, carcass composition and muscular protein synthesis-related genes expression in suckling piglets. Sixteen piglets of a crossbred genotype[Duroc×(Landrace×Large White)] were obtained from 4 litters in 4 successive replicates and grouped into 8 pairs according to sex and body weight. At 4 days of age, piglets within each pair were offered the same amount of feed allowance either in 6 (M6 group, n=8) or 12 (M12 group, n=8) meals per day during a 3-week interventional period. The results showed as follows:1) compared with M12 group, the final body weight and average daily body weight at week 2, week 3 and during the whole trial in M6 group significantly increased (P<0.05), and the feed/gain at week 2, week 3 and during the whole trial significantly decreased (P<0.05). 2) Compared with M12 group, the carcass lean percentage and weight of longissimus muscle and semitendinosus muscle in M6 group significantly increased (P<0.05). 3) Compared with M12 group, the mRNA expression of insulin-like growth factor 1 (IGF1R) in M6 group had an increasing trend (P=0.05); compared with M12 group, the mRNA expression of insulin-like growth factor 1 receptor (IGF1R), mammalian target of rapamcin (mTOR), eukaryotic cells translate initiation factor 4E (EIF4E) and ribosomal protein S6 (RPS6) in M6 group significantly increased (P<0.05), and the mRNA expression of Ⅱ type B activated receptor (ACVR2B) and type Ⅰ activated kinase receptor (ALK5) in M6 group significantly decreased (P<0.05); compared with M12 group, the mRNA expression of myostatin (MSTN) in M6 group had no significant change (P>0.05). Collectively, reduced meal frequency results in accelerated carcass lean deposition and muscle hypotrophy, which may be mediated by activated IGF1-IGF1R-mTOR pathway and down-regulated expression of MSTN receptors.

Key words： meal frequency; growth performance; skeletal muscle; protein synthesis; piglets

Cite this article

LIU Jingbo, CAO Shanchuan, YANG Yong, ZHANG Hongfu . Effects of Meal Frequency on Growth Performance and Muscle Protein Synthesis in Suckling Piglets[J]. Chinese Journal of Animal Nutrition, 2019 , 31(7) : 3049 -3057 . DOI: 10.3969/j.issn.1006-267x.2019.07.015

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