Difference in Recoveries of Digestive Enzyme Activities during Purification Process for Jejunal Fluid of Pigs in Different Growth Phases and Diet Types

WANG Yuming, WANG Ya, DANG Fangkun, ZHANG Hu, GAO Qingtao, ZHAO Feng

doi:10.3969/j.issn.1006-267x.2019.07.017

2019 , Vol. 31 >Issue 7: 3067 - 3073

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.017

Molecular Nutrition

Difference in Recoveries of Digestive Enzyme Activities during Purification Process for Jejunal Fluid of Pigs in Different Growth Phases and Diet Types

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agriculture Science, Beijing 100193, China;
2. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Chinese Agricultural University, Beijing 100193, China

Received date: 2018-12-19

Online published: 2019-07-19

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Abstract

This study was conducted to investigate the difference in the recovery of digestive enzyme activity of different jejunal fluid sources during purification process in pigs, to provide reference for the preparation of digestive enzyme in vitro. Two growth phases[growing period (60 to 100 days of age) or fattening period (101 to 140 days of age)] and two diets[corn-soybean meal diet or corn-corn distillers dried grains with soluble (DDGS)-rice bran meal diet] were used in a 2×2 factorial arrangement in a completely randomized design. Eight growing crossbred barrows (Yorkshire×Landrace) surgically fitted with permanent T-cannula in the upper jejunum were randomly divided into 2 groups in a 2×2 change-over design with 4 pigs per group. Each phase contained 2 experimental periods and 2 diets were cross feeding with 8 pigs per treatment. Each experimental period lasted for 10 d acclimatization and 3 d jejunal fluid collection. The jejunal fluid was pooled of each pig after collection, and then purified by anhydrous ethanol precipitated. The loss and recovery of digestive enzyme activity were determined during the purification process. The results showed as follows:1) there was no significant difference in the recovery of digestive enzyme activity among different sources of jejunal fluid after ice ethanol precipitated-redissolved process (P>0.05), and the recovery of enzyme activity was more than 70%. 2) No significant difference was observed in the recovery of digestive enzyme activity among different sources of jejunal fluid after ice ethanol precipitated-redissolved-freeze dried process (P>0.05), and the recovery of enzyme activity was more than 60%. 3) The loss rate of digestive enzyme activity in the precipitated-redissolved process was significantly higher than that in the freeze-dried process (P<0.01). In conclusion, no significant difference in the recovery of digestive enzyme activity in the purification process of jejunal fluid observed from pigs under different growing phases and diet types, and satisfactory recovery can be obtained using anhydrous ethanol precipitation.

Key words： jejunal fluid; purified; digestive enzyme activity; recovery; growing pigs

Cite this article

WANG Yuming, WANG Ya, DANG Fangkun, ZHANG Hu, GAO Qingtao, ZHAO Feng . Difference in Recoveries of Digestive Enzyme Activities during Purification Process for Jejunal Fluid of Pigs in Different Growth Phases and Diet Types[J]. Chinese Journal of Animal Nutrition, 2019 , 31(7) : 3067 -3073 . DOI: 10.3969/j.issn.1006-267x.2019.07.017

References

[1] YEGANI M,KORVER D R.Effects of corn source and exogenous enzymes on growth performance and nutrient digestibility in broiler chickens[J].Poultry Science,2013,92(5):1208-1220.

[2] GIUBERTI G,GALLO A,CERIOLI C,et al.In vitro starch digestion and predicted glycemic index of cereal grains commonly utilized in pig nutrition[J].Animal Feed Science and Technology,2012,174(3/4):163-173.

[3] FURUYA S,SAKAMOTO K,TAKAHASHI S.A new in vitro method for the estimation of digestibility using the intestinal fluid of the pig[J].British Journal of Nutrition,1979,41(3):511-520.

[4] 张子仪,聂光达.猪饲料消化能值的离体测定方法及其生物学试验根据[J].中国畜牧杂志,1986(1):6-10.

[5] 胡光源.生长猪小肠仿生消化试剂设计依据的研究[D].硕士学位论文.北京:中国农业科学院,2010.

[6] 严峰.酶的组成及储存条件对模拟鸭肠液消化能力的影响[D].硕士学位论文.武汉:华中农业大学,2012.

[7] 张莉.基于鸭源性消化酶粉剂的模拟鸭肠液制备方法的研究[D].硕士学位论文.北京:中国农业科学院,2014.

[8] 刘成玲,赵峰,左建军,等.生长猪回肠末端瘘管中食糜养分流量及指示剂回收率的变异[J].动物营养学报,2014,26(11):3404-3413.

[9] NRC.Nutrient requirements of swine[S].Washington,D.C.:National Academies Press,2012.

[10] DAHLQVIST A.A method for the determination of amylase in intestinal content[J].Scandinavian Journal of Clinical and Laboratory Investigation,1962,14(2):145-151.

[11] WIRNT R.Trypsin,measurement with N-α-p-tolue-nesulfony-L-arginine methyl ester as substrate[M]//BERGMEYER H U.Methods of enzymatic analysis.Weinheinm,Germany:Verlag Chemie,1974:1021-1024.

[12] WIRNT R.Chymotrypsin,measurements with N-benzoyl-L-tyrosin methyl ester as substrate[M]//BERGMEYER H U.Methods of enzymatic analysis.Weinheinm:Verlag Chemie,1974:1009-1012.

[13] 刘婧.猪血超氧化物歧化酶的提取、性质及其化学修饰研究[D].硕士学位论文.长春:吉林大学,2011.

[14] 吴晓英,张聚宝,林影,等.胰酶制备新工艺的研究[J].广东药学院学报,2005,21(1):64-67.

[15] 安正仑,高学军,孙福忠.猪胰酶制备工艺研究[J].黑龙江畜牧兽医,2006(11):74-75.

[16] 郭兆斌,韩玲,刘亮亮,等.猪胰脏中胰酶的提取工艺优化研究[J].食品科学,2009,30(22):162-164.

[17] ZHA S H,ZHAO Q S,CHEN J J,et al.Extraction,purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii)[J].Carbohydrate Polymers,2014,111:584-587.

[18] TRENTINI M M S,TONIAZZO G,ZENI J,et al.Purification of pectinases from Aspergillus niger ATCC 9642 by ethanol precipitation[J].Biocatalysis and Agricultural Biotechnology,2015,4(3):315-320.

[19] WANG L H,HARTMAN P A.Purification and some properties of an extracellular maltase from Bacillus subtilis[J].Applied and Environmental Microbiology,1976,31(1):108-118.

[20] 郑先哲,汪春,贾富国.农产品干燥理论与技术[M].北京:中国轻工业出版社,2009.

[21] 张琪,朱庆锋,崔百元,等.喷雾干燥法制备饲用植酸酶制剂[J].吉林农业大学学报,2016,38(6):750-753.

[22] 薛菲,王凤山.蛋白质的冻干保护剂及其保护机制研究进展[J].中国药学杂志,2018,53(10):765-770.

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