This experiment was conducted to investigate the dietary lysine requirement for Chinese yellow-feather broilers from 1 to 63 days of age in different growth stages. A 2 (gender)×5 (lysine level) factor test design was adopted in the experiment, and the fast Lingnan yellow chickens of 1, 22 and 43 days of age (slaughtered at 63 days of age) were selected for feeding with three stages of 21 days each. In experiment 1, a total of 750 male and 750 female 1-day-old chickens were selected, and divided into 5 male groups and 5 female groups with 3 replicates in each group and 50 chickens in each replicate. In addition, 120 males and 120 females were selected and divided into 5 male groups and 5 female groups with 3 replicates in each group and 8 chickens in each replicate for the nitrogen metabolism test. In experiment 2, a total of 800 male and 800 female 22-day-old broilers were selected, and divided into 5 male groups and 5 female groups with 4 replicates in each group and 40 broilers in each replicate. In addition, 120 males and 120 females were selected and divided into 5 male groups and 5 female groups with 4 replicates in each group and 6 broilers in each replicate for the nitrogen metabolism test. In experiment 3, a total of 800 male and 800 female 43-day-old broilers were selected and grouped with the same of experiment 2. The dietary lysine levels were set as 0.93%, 1.03%, 1.13%, 1.23% and 1.33% for 1 to 21 days of age, 0.80%, 0.90%, 1.00%, 1.10% and 1.20% for 22 to 42 days of age, and 0.65%, 0.75%, 0.85%, 0.95% and 1.05% for 43 to 63 days of age. The effects of dietary lysine levels on growth performance, carcass quality, nitrogen metabolism rate and serum biochemical indices of broilers were measured to determine the dietary lysine requirement for Chinese yellow-feather broilers with different genders and ages. The results showed that the average daily weight gain of both male and female broilers from 1 to 21 days was the highest, and feed to gain ratio and serum uric acid content were the lowest in 1.13% lysine level group. From 22 to 42 days of age, the feed to gain ratio of male broilers in 1.00% lysine level group was relatively low, and the average daily weight of both female and male broilers showed a regular trend of increasing first and then decreasing with the dietary lysine level increasing, and the female broilers performed the best and had the highest breast muscle rate when the dietary lysine level was 1.00%. From 43 to 63 days of age, the average daily gain of both male and female broilers was the highest, the feed to gain ratio was lower, and the breast muscle rate of female broilers was the highest in 0.85% lysine level group. In conclusion, under the conditions of this experiment, the optimal dietary lysine levels of Chinese yellow-feather broilers at the ages of 1 to 21 days, 22 to 42 days and 43 to 63 days are 1.13%, 1.00% and 0.85%, respectively.