Effects of Alanyl-Glutamine Dipeptide Supplementation on Growth Performance, Antioxidant Status, Immune Response and Stress Resistance of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

LI Xue, ZHANG Muzi, LI Ming, ZHANG Qian, WANG Rixin, JIANG Haibo

doi:10.3969/j.issn.1006-267x.2019.07.031

2019 , Vol. 31 >Issue 7: 3197 - 3206

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.031

Molecular Nutrition

Effects of Alanyl-Glutamine Dipeptide Supplementation on Growth Performance, Antioxidant Status, Immune Response and Stress Resistance of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

Expand

1. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. College of Animal Sciences, Guizhou University, Guiyang 550025, China;
3. Key Laboratory for Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region of Ministry of Education, Guizhou University, Guiyang 550025, China

Received date: 2018-12-14

Online published: 2019-07-19

Fold

Abstract

A 56-day feeding trial was conducted to determine the alanyl-glutamine dipeptide (AGD) supplementation on growth performance, antioxidant status, immune response and stress resistance of juvenile yellow catfish (Pelteobagrus fulvidraco). Juvenile yellow catfish with an average body weight of (1.98±0.01) g were randomly assigned to 5 groups with 3 replicates per group and 30 juvenile fish per replicate. Juvenile fish in the 5 groups were fed 5 experimental diets supplemented with 0 (control) 0.25%, 0.50%, 0.75% and 1.00% AGD, and the measured AGD contents in the 5 experimental diets were 0.08%, 0.23%, 0.56%, 0.69% and 1.09%, respectively. The results showed that the weight gain, specific growth rate, serum total protein, albumin, globulin, complement 3, complement 4 contents and superoxide dismutase, catalase, glutathione, lysozyme, alkaline phosphatase, acid phosphatase activities were firstly increased and then increased with dietary AGD supplemental level increasing, and above indexes in 0.50% and 0.75%AGD groups were significantly higher than those in control group (P<0.05). Diet supplemented different levels of AGD could significantly increase the serum total antioxidant capacity (P<0.05), but significantly decrease the serum malondialdehyde content (P<0.05). After 96 h post-challenge on ammonia exposure, the cumulative mortality, brain ammonia and glutamate contents in control group were significantly higher than those in each AGD supplementation groups (P<0.05). In conclusion, suitable level of AGD supplemented into diets can increase the growth performance, antioxidant status, immune response and stress resistance of juvenile yellow catfish. The optimal dietary AGD supplemental level is estimated to be 0.56% in the diet of juvenile yellow catfish by quadratic regression analysis base on specific growth rate. By a comprehensive consideration of growth performance, antioxidant status, immune response and stress resistance, the supplemental level of 0.50% to 0.75% AGD is recommended in diets of juvenile yellow catfish.

Key words： alanyl-glutamine dipeptide; juvenile yellow catfish; growth performance; antioxidant status; immune response; stress resistance

Cite this article

LI Xue, ZHANG Muzi, LI Ming, ZHANG Qian, WANG Rixin, JIANG Haibo . Effects of Alanyl-Glutamine Dipeptide Supplementation on Growth Performance, Antioxidant Status, Immune Response and Stress Resistance of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(7) : 3197 -3206 . DOI: 10.3969/j.issn.1006-267x.2019.07.031

References

[1] FELIG P.Amino acid metabolism in man[J].Annual Review of Biochemistry,1975,44:933-955.

[2] XU H,ZHU Q,WANG C A,et al.Effect of dietary alanyl-glutamine supplementation on growth performance,development of intestinal tract,antioxidant status and plasma non-specific immunity of young mirror carp (Cyprinus carpio L.)[J].Journal of Northeast Agricultural University,2014,21(4):37-46.

[3] CRUZAT V F,TIRAPEGUI J.Effects of oral supplementation with glutamine and alanyl-glutamine on glutamine,glutamate,and glutathione status in trained rats and subjected to long-duration exercise[J].Nutrition,2009,25(4):428-435.

[4] CURI R,LAGRANHA C J,DOI S Q,et al.Molecular mechanisms of glutamine action[J].Journal of Cellular Physiology,2005,204(2):392-401.

[5] SCHRÖDER J,WARDELMANN E,WINKLER W,et al.Glutamine dipeptide-supplemented parenteral nutrition reverses gut atrophy,disaccharidase enzyme activity,and absorption in rats[J].Journal of Parenteral and Enteral Nutrition,1995,19(6):502-506.

[6] RENNIE M J,BOWTELL J L,BRUCE M,et al.Interaction between glutamine availability and metabolism of glycogen,tricarboxylic acid cycle intermediates and glutathione[J].The Journal of Nutrition,2001,131(9):2488S-2490S.

[7] LIN Y,XIAO Q Z.Dietary glutamine supplementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture,2006,256(1/2/3/4):389-394.

[8] CHENG Z Y,BUENTELLO A,GATLIN Ⅲ D M.Effects of dietary arginine and glutamine on growth performance,immune responses and intestinal structure of red drum,Sciaenops ocellatus[J].Aquaculture,2011,319(1/2):247-252.

[9] QIYOU X,QING Z,HONG X,et al.Dietary glutamine supplementation improves growth performance and intestinal digestion/absorption ability in young hybrid sturgeon (Acipenser schrenckii ♀×Huso dauricus)[J].Journal of Applied Ichthyology,2011,27(2):721-726.

[10] POHLENZ C,BUENTELLO A,CRISCITIELLO M F,et al.Synergies between vaccination and dietary arginine and glutamine supplementation improve the immune response of channel catfish against Edwardsiella ictaluri[J].Fish & Shellfish Immunology,2012,33(3):543-551.

[11] ZHANG K K,MAI K S,XU W,et al.Effects of dietary arginine and glutamine on growth performance,nonspecific immunity,and disease resistance in relation to arginine catabolism in juvenile turbot (Scophthalmus maximus L.)[J].Aquaculture,2017,468:246-254.

[12] BRITO G A C,CARNEIRO-FILHO B,ORIÁ R B,et al.Clostridium difficile toxin A induces intestinal epithelial cell apoptosis and damage:role of Gln and Ala-Gln in toxin A effects[J].Digestive Diseases and Sciences,2005,50(7):1271-1278.

[13] 农业部渔业渔政管理局.2017中国渔业统计年鉴[M].北京:中国农业出版社,2017.

[14] JIANG J,XU S X,FENG L,et al.Lysine and methionine supplementation ameliorates high inclusion of soybean meal inducing intestinal oxidative injury and digestive and antioxidant capacity decrease of yellow catfish[J].Fish Physiology and Biochemistry,2018,44(1):319-328.

[15] SONG Y F,LUO Z,PAN Y X,et al.Three unsaturated fatty acid biosynthesis-related genes in yellow catfish Pelteobagrus fulvidraco:molecular characterization,tissue expression and transcriptional regulation by leptin[J].Gene,2015,563(1):1-9.

[16] LUO Z,WEI C C,YE H M.Effect of dietary choline levels on growth performance,lipid deposition and metabolism in juvenile yellow catfish Pelteobagrus fulvidraco[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2016,202:1-7.

[17] CHEN G H,HOGSTRAND C,LUO Z,et al.Dietary zinc addition influenced zinc and lipid deposition in the fore-and mid-intestine of juvenile yellow catfish Pelteobagrus fulvidraco[J].British Journal of Nurtrition,2017,118(8):570-579.

[18] LI M,CHEN L Q,QIN J G,et al.Growth performance,antioxidant status and immune response in darkbarbel catfish Pelteobagrus vachelli fed different PUFA/vitamin E dietary levels and exposed to high or low ammonia[J].Aquaculture,2013,406-407:18-27.

[19] AOAC.Official methods of analysis of AOAC International[S].Gaithersburg,Maryland:Association of Official Analytical Chemists,2000.

[20] 叶应妩,王毓三,申子瑜.全国临床检验操作规程[M].3版.南京:东南大学出版社,2006:595-606.

[21] BERGMEYER H U.Methods of enzymatic analysis[M].New York:Academic Press,1985:454-461.

[22] IP Y K,LEONG M W,SIM M Y,et al.Chronic and acute ammonia toxicity in mudskippers,Periophthalmodon schlosseri and Boleophthalmus boddaerti:brain ammonia and glutamine contents,and effects of methionine sulfoximine and MK801[J].Journal of Experimental Biology,2005,208(10):1993-2004.

[23] MILLWARD D J,JEPSON M M,OMER A.Muscle glutamine concentration and protein turnover in vivo in malnutrition and in endotoxemia[J].Metabolism,1989,38(Suppl.1):6-13.

[24] CHEN X M,GUO G L,SUN L,et al.Effects of Ala-Gln feeding strategies on growth,metabolism,and crowding stress resistance of juvenile Cyprinus carpio var. Jian[J].Fish & Shellfish Immunology,2016,51:365-372.

[25] DING Z K,LI W F,HUANG J H,et al.Dietary alanyl-glutamine and vitamin E supplements could considerably promote the expression of GPx and PPARα genes,antioxidation,feed utilization,growth,and improve composition of juvenile cobia[J].Aquaculture,2017,470:95-102.

[26] ROGERO M M,TIRAPEGUI J,PEDROSA R G,et al.Effect of alanyl-glutamine supplementation on plasma and tissue glutamine concentrations in rats submitted to exhaustive exercise[J].Nutrition,2006,22(5):564-571.

[27] ANDERSON P M,BRODERIUS M A,FONG K C,et al.Glutamine synthetase expression in liver,muscle,stomach and intestine of Bostrichthys sinensis in response to exposure to a high exogenous ammonia concentration[J].Journal of Experimental Biology,2002,205:2053-2065.

[28] POHLENZ C,BUENTELLO A,BAKKE A M,et al.Free dietary glutamine improves intestinal morphology and increases enterocyte migration rates,but has limited effects on plasma amino acid profile and growth performance of channel catfish Ictalurus punctatus[J].Aquaculture,2012,370-371:32-39.

[29] HAN Y Z,KOSHIO S,JIANG Z Q,et al.Interactive effects of dietary taurine and glutamine on growth performance,blood parameters and oxidative status of Japanese flounder Paralichthys olivaceus[J].Aquaculture,2014,434:348-354.

[30] COUTINHO F,CASTRO C,RUFINO-PALOMARES E,et al.Dietary glutamine supplementation effects on amino acid metabolism,intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2016,191:9-17.

[31] GU M,BAI N,XU B Y,et al.Protective effect of glutamine and arginine against soybean meal-induced enteritis in the juvenile turbot (Scophthalmus maximus)[J].Fish & Shellfish Immunology,2017,70:95-105.

[32] LIU J W,MAI K S,XU W,et al.Effects of dietary glutamine on survival,growth performance,activities of digestive enzyme,antioxidant status and hypoxia stress resistance of half-smooth tongue sole (Cynoglossus semilaevis Günther) post larvae[J].Aquaculture,2015,446:48-56.

[33] CHEN J,ZHOU X Q,FENG L,et al.Effects of glutamine on hydrogen peroxide-induced oxidative damage in intestinal epithelial cells of Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture,2009,288(3/4):285-289.

[34] LUO M H,BAZARGAN N,GRIFFITH D P,et al.Metabolic effects of enteral versus parenteral alanyl-glutamine dipeptide administration in critically ill patients receiving enteral feeding:a pilot study[J].Clinical Nutrition,2008,27(2):297-306.

[35] DOGUET F,TAMION F,LE GUILLOU V,et al.Albumin limits mesenteric endothelial dysfunction and inflammatory response in cardiopulmonary bypass[J].Artificial Organs,2012,36(11):962-971.

[36] FLAJNIK M F,KASAHARA M.Origin and evolution of the adaptive immune system:genetic events and selective pressures[J].Nature Reviews Genetics,2010,11(1):47-59.

[37] CHEN H Y,MILLER P S,LEWIS A J,et al.Changes in plasma urea concentration can be used to determine protein requirements of two populations of pigs with different protein accretion rates[J].Journal of Animal Science,1995,73(9):2631-2639.

[38] SAURABH S,SAHOO P K.Lysozyme:an important defence molecule of fish innate immune system[J].Aquaculture Research,2008,39(3):223-239.

[39] BARTELL S M,BATAL A B.The effect of supplemental glutamine on growth performance,development of the gastrointestinal tract,and humoral immune response of broilers[J].Poultry Science,2007,86(9):1940-1947.

[40] SHAH N J,NEEB H,KIRCHEIS G,et al.Quantitative cerebral water content mapping in hepatic encephalopathy[J].Neuroimage,2008,41(3):706-717.

[41] ROSE C.Increased extracellular brain glutamate in acute liver failure:decreased uptake or increased release?[J].Metabolic Brain Disease,2002,17(4):251-261.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References