Contribution of Digestive Enzymes in Simulated Digestion of Small Intestine to Digestibility of Gross Energy and Crude Protein of Feed for Pigs

WANG Ya, ZHAO Feng, ZHANG Hu, DANG Fangkun, GAO Qingtao, YU Yao, DU Zhongyuan, SA Renna

doi:10.3969/j.issn.1006-267x.2019.07.036

2019 , Vol. 31 >Issue 7: 3242 - 3250

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.036

Molecular Nutrition

Contribution of Digestive Enzymes in Simulated Digestion of Small Intestine to Digestibility of Gross Energy and Crude Protein of Feed for Pigs

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State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2018-12-19

Online published: 2019-07-19

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Abstract

This study was conducted to investigate the contribution of composition and activity of main digestive enzymes in simulated digestion of the small intestine to the digestibility of nutrients in feed for pig, which will provide a basis to improve the procedure of simulated digestion for determining nutrient digestibility of feed for pig. Five treatments consisted of gastric simulated digestion, gastric following amylase for small intestinal simulated digestion, gastric following amylase and chymotrypsin for intestinal simulated digestion, gastric following amylase, chymotrypsin and trypsin for intestinal simulated digestion, gastric following amylase, chymotrypsin, trypsin and supplemented digestive enzymes at 4 h for intestinal simulated digestion were arranged in a single factor completely randomized design. Each treatment contained 5 replicates with 1 digestion tube in each. The differences in the digestibility of dry matter, crude protein and gross energy and the contribution of each digestive enzyme to nutrient digestibility were investigated among various treatment conditions for each of corn, soybean meal, wheat bran and corn-soybean meal diet. The results showed as follows:1) amylase and chymotrypsin in simulated intestinal fluid could significantly increase the digestibilityof dry matter, crude protein and gross energy of corn, soybean meal, wheat bran and corn-soybean meal diet digested by simulated gastric fluid (P<0.05). Trypsin could further increase the digestibility of dry matter, crude protein and gross energy of soybean meal and wheat bran digested by simulated gastric fluid (P<0.05). The supplement of 3 digestive enzymes at 4 h of intestinal digestion could increase the crude protein digestibility of wheat bran, and the digestibility of dry matter, crude protein and gross energy of corn-soybean meal (P<0.05). 2) The contribution of digestive enzymes in the small intestinal fluid to the digestibility of feed nutrients varied depending on the feed substrate. Amylase and chymotrypsin had relative greater contribution to the digestibility of gross energy and crude protein contrast to trypsin and supplemented digestive enzymes. However, trypsin and supplemented digestive enzymes also significantly increased the nutrient digestibility (P<0.05). In the simulation of small intestine digestion for pigs, amylase, chymotrypsin, trypsin and supplemental digestive enzymes have significant contribution to the digestion of feed nutrients.

Key words： pig; simulated digestion; digestive enzyme; nutrient digestibility

Cite this article

WANG Ya, ZHAO Feng, ZHANG Hu, DANG Fangkun, GAO Qingtao, YU Yao, DU Zhongyuan, SA Renna . Contribution of Digestive Enzymes in Simulated Digestion of Small Intestine to Digestibility of Gross Energy and Crude Protein of Feed for Pigs[J]. Chinese Journal of Animal Nutrition, 2019 , 31(7) : 3242 -3250 . DOI: 10.3969/j.issn.1006-267x.2019.07.036

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