Effects of Dietary Neutral Detergent Fiber to Starch Ratio on Methane Production Using in Vitro Gas Production Technique

WANG Zenglin, SUN Xueli, LIU Taotao, LI Qiufeng, CAO Yufeng, NU Jiankang, WANG Meimei, CHEN Panliang, BAI Dayang

doi:10.3969/j.issn.1006-267x.2019.07.037

2019 , Vol. 31 >Issue 7: 3251 - 3259

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.037

Molecular Nutrition

Effects of Dietary Neutral Detergent Fiber to Starch Ratio on Methane Production Using in Vitro Gas Production Technique

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College of Animal Science and Technology, Hebei Agriculture University, Baoding 071001, China

Received date: 2018-12-23

Online published: 2019-07-19

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Abstract

This experiment was conducted to study the effects of dietary neutral detergent fiber to starch ratio (NDF/starch) on rumen methane production using in vitro gas production technique. The single factor experiment was screened for the optimal NDF/starch. The NDF/starch in 15 treatments were 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.2 and 2.3, with 5 replicates in each treatment, respectively. In vitro gas production method was performed to analyze gas production, methane concentration and methane production at 24 and 48 h. The results showed as follows:1) the gas production of dietary NDF/starch between 0.8 and 1.2 was higher, methane production was lower. The methane yield of dietary NDF/starch of 1.2 was the lowest. 2) Dietary NDF and starch at different ratios had significant effects on gas production, methane concentration and methane production (P<0.05 or P<0.01). 3) The linear regression analysis showed that significantly positive relationships were found between dietary NDF/starch and the methane concentration and production at 24 and 48 h (P<0.01), and significantly negative relationships were found between dietary NDF/starch and the gas production at 24 and 48 h (P<0.01). 4) The linear regression analysis showed that significantly positive relationships was found between the methane production at 24 h and acetic acid/propionic acid (P<0.05). It was showed that significantly positive relationships were found between the methane production at 24 and 48 h, the methane concentration at 48 h. Significantly negative relationships were found between dietary NDF/starch and ammonia nitrogen (NH₃-N) concentration (P<0.01). The methane production at 24 and 48 h, dietary NDF/starch were strongly related to parameters in vitro, regression coefficients were 0.942, 0.982 and 0.975, respectively. It is concluded that dietary NDF/starch between 0.8 and 1.2 can promote methane emission reduction, and the effect is the best when dietary NDF/starch is 1.2 under the present experimental conditions. The high regression coefficients indicate that in vitro methane production can be predicted merely based dietary NDF/starch.

Key words： in vitro gas production technique; dietary neutral detergent fiber to starch ratio; rumen; methane; fermentation parameters

Cite this article

WANG Zenglin, SUN Xueli, LIU Taotao, LI Qiufeng, CAO Yufeng, NU Jiankang, WANG Meimei, CHEN Panliang, BAI Dayang . Effects of Dietary Neutral Detergent Fiber to Starch Ratio on Methane Production Using in Vitro Gas Production Technique[J]. Chinese Journal of Animal Nutrition, 2019 , 31(7) : 3251 -3259 . DOI: 10.3969/j.issn.1006-267x.2019.07.037

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