Molecular Nutrition

Effects of Bacillus subtilis on Plasma Biochemical and Immune Indices and Fecal Microflora of Finishing Pigs

Expand
  • 1. Hunan Co-Innovation Center of Animal Production Safety, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Asahi Calpis Wellness(Shanghai) Co., Ltd., Shanghai 200233, China;
    3. Shanghai Naseco Products Company, Shanghai 201700, China

Received date: 2018-12-04

  Online published: 2019-07-19

Abstract

The aim of this study was to investigate the effects of Bacillus subtilis (BS) on plasma biochemical and immune indices and fecal microflora of finishing pigs. A total of 120 Duroc×Landrace×Yorkshire crossbred finishing pigs with an average body weight of (62.90±2.37) kg were randomly divided into 3 groups with 5 replicates per group and 8 pigs per replicate. The control group (group A) was fed a basal diet, and the experimental groups (groups B and C) were fed the basal diets supplemented with 100 and 200 mg/kg BS, receptivity. The preparation period was 7 days and formal experiment period was 63 days. The results showed as follows:1) compared with the control group, the plasma alkaline phosphatase activity of finishing pigs in groups B and C was extremely significantly decreased (P<0.01); the plasma globulin content of finishing pigs in group B was significantly decreased (P<0.05) and the plasma albumin content was significantly increased (P<0.05). 2) The plasma immunoglobulin A content and porcine circovirus antibody level of finishing pigs in group C were extremely significantly higher than those in control group (P<0.01). 3) The addition of Bacillus subtilis in diets had no significant effect on the endotoxin content in plasma and muscle of finishing pigs (P>0.05). 4) Compared with the control group, the relative content of Bifidobacteria in faeces of finishing pigs in groups B and C was significantly increased (P<0.05), the relative content of Bacillus megaterium in faeces of finishing pigs in group B was significantly increased (P<0.05), and the relative content of Clostridium perfringens in faeces of finishing pigs in group C was extremely significantly decreased (P<0.01). It can be seen that adding BS to diet can improve metabolism, enhance immune function and optimize intestinal flora of finishing pigs to a certain extent.

Cite this article

HE Changqing, ZHU Jin, KUANG Youhua, CHEN Jifa, QU Xiangyong, GUO Songchang, JINLIN Kuanhe, ZHOU Xuebin . Effects of Bacillus subtilis on Plasma Biochemical and Immune Indices and Fecal Microflora of Finishing Pigs[J]. Chinese Journal of Animal Nutrition, 2019 , 31(7) : 3260 -3267 . DOI: 10.3969/j.issn.1006-267x.2019.07.038

References

[1] BARTON M D.Impact of antibiotic use in the swine industry[J].Current Opinion in Microbiology,2014,19:9-15.

[2] 王晓阁.枯草芽孢杆菌研究进展与展望[J].中山大学研究生学刊(自然科学、医学版),2012,33(3):14-23.

[3] 苏海燕.枯草芽孢杆菌制剂对仔猪生长性能的影响[D].硕士学位论文.长沙:湖南农业大学,2010.

[4] GUO J R,DOND X F,LIU S,et al.Effects of long-term Bacillus subtilis CGMCC 1.921 supplementation on performance,egg quality,and fecal and cecal microbiota of laying hens[J].Poultry Science,2017,96(5):1280-1289.

[5] AFRC R F.Probiotics in man and animals[J].Journal of Applied Bacteriology,2010,66(5):365-378.

[6] OH J K,PAJARILLO E A B,CHAE J P,et al.Protective effects of Bacillus subtilis against Salmonella infection in the microbiome of Hy-Line brown layers[J].Asian-Australasian Journal of Animal Sciences,2017,30(9):1332-1339.  

[7] 陈继发,匡佑华,康克浪,等.蒙脱石、枯草芽孢杆菌及其互作对蛋鸡血浆激素含量、卵巢繁殖相关基因表达和肠道微生物的影响[J].动物营养学报,2019,31(1):294-303.

[8] CHEN X L,WANG J K,WU Y M,et al.Effects of chemical treatments of rice straw on rumen fermentation characteristics,fibrolytic enzyme activities and populations of liquid-and solid-associated ruminal microbes in vitro[J].Animal Feed Science and Technology,2008,141(1/2):1-14.

[9] 杜恩存.百里香酚和香芹酚对肉仔鸡肠上皮屏障和免疫功能的调节作用[D].博士学位论文.北京:中国农业大学,2016.

[10] 尹清强,李小飞,常娟,等.微生态制剂对哺乳和断奶仔猪生产性能的影响及作用机理研究[J].动物营养学报,2011,23(4):622-630.

[11] FORTE C,MOSCATI L,ACUTI G,et al.Effects of dietary Lactobacillus acidophilus and Bacillus subtilis on laying performance,egg quality,blood biochemistry and immune response of organic laying hens[J].Journal of Animal Physiology and Animal Nutrition,2016,100(5):977-987.  

[12] LI W F,RAJPUT I R,XU X,et al.Effects of probiotic (Bacillus subtilis) on laying performance,blood biochemical properties and intestinal microflora of Shaoxing duck[J].International Journal of Poultry Science,2011,10(8):583-589.  

[13] MA Q G,GAO X,ZHOU T,et al.Protective effect of Bacillus subtilis ANSB060 on egg quality,biochemical and histopathological changes in layers exposed to aflatoxin B1[J].Poultry Science,2012,91(11):2852-2857.  

[14] 吕尊周,袁肖笑,郝柱,等.芽孢杆菌制剂对蛋鸡产蛋性能、血清相关指标及肠道菌群的影响[J].中国兽医学报,2012,32(7):1068-1072.

[15] 杨锋.枯草芽孢杆菌的抗逆特性及其对仔猪生化指标和氨气排放的影响[D].硕士学位论文.杭州:浙江工商大学,2011.

[16] 杨清丽,彭豫东,曲湘勇,等.纳米硒对产蛋鸡生产性能、血清免疫和生化指标及蛋黄中硒含量的影响[J].动物营养学报,2017,29(1):280-289.

[17] 易丹.枯草芽孢杆菌与三肽囊素对肉鸡生长性能、血液理化、免疫功能及肠道菌群的影响[D].硕士学位论文.雅安:四川农业大学,2014.

[18] SUN P,WANG J Q,ZHANG H T.Effects of Bacillus subtilis natto on performance and immune function of preweaning calves[J].Journal of Dairy Science,2010,93(12):5851-5855.  

[19] VITIÑI E,ALVAREZ S,MEDINA M,et al.Gut mucosal immunostimulation by lactic acid bacteria[J].Biocell,2000,24(3):223-232.

[20] 陈继发,朱瑾,曲湘勇.枯草芽孢杆菌的作用机制及其在家禽生产中的应用[J/OL].经济动物学报,1-7.(2018-09-14)[2018-12-25].https://doi.org/10.13326/j.jea.2018.1284.

[21] 张超.复合微生态制剂对断乳仔猪生长速度及猪瘟抗体水平影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2012.

[22] LIU X,PENG C Y,QU X Y,et al.Effects of Bacillus subtilis C-3102 on production,hatching performance,egg quality,serum antioxidant capacity and immune response of laying breeders[J].Journal of Animal Physiology Animal Nutrition,2019,103(1):182-190.  

[23] 郭利伟.中药多糖复方免疫增强剂AEI及其作用机理研究[D].博士学位论文.南京:南京农业大学,2012.

[24] 于涟,李建荣,黄耀伟,等.鸡白细胞介素2增强传染性法氏囊病病毒多聚蛋白DNA疫苗免疫原性的研究[J].生物工程学报,2001,17(6):652-657.

[25] 汪志,董国忠,吴剑波.内毒素对猪的危害及其控制[J].动物营养学报,2017,29(2):397-402.

[26] LUDIDI S,JONKERS D,ELAMIN E,et al.The intestinal barrier in irritable bowel syndrome:subtype-specific effects of the systemic compartment in an in vitro model[J].PLoS One,2015,10(5):e0123498.

[27] LI F Z,LI S W,LIU X F,et al.The structure of WbnH in a near active state[J].Protein & Cell,2015,6(8):615-618.  

[28] 李先根,涂治骁,王树辉,等.亚麻籽油对脂多糖刺激断奶仔猪肠黏膜结构和免疫细胞的影响[J].动物营养学报,2018,30(2):515-523.

[29] 余嘉瑶,李毅,王恬,等.日粮中添加姜黄素对免疫应激断奶仔猪肠道抗氧化能力及糖代谢的影响[J].畜牧与兽医,2018,50(5):51-55.

[30] 赵迪.益生菌对脂多糖刺激仔猪组织中氧化相关指标和有关基因表达的影响[D].硕士学位论文.武汉:武汉轻工大学,2014.

[31] JAYARAMAN S,THANGAVEL G,KURIAN H,et al.Bacillus subtilis PB6 improves intestinal health of broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis[J].Poultry Science,2013,92(2):370-374.  

[32] LI Z,WANG W,LV Z,et al.Bacillus subtilis and yeast cell wall improve the intestinal health of broilers challenged by Clostridium perfringens[J].British Poultry Science,2017,58(6):635-643.  

[33] 曾敏.可速必宁的耐药性能及对猪生长性能的影响研究[D].硕士学位论文.长沙:湖南农业大学,2015.

[34] GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science,2010,129(1/2/3):95-103.

[35] GUO X H,LI D F,LU W Q,et al.Screening of bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs[J].Antonie van Leeuwenhoek,2006,90(2):139-146.  

[36] MANEEWAN C,YAMAUCHI K E,THIRABUNYANON M,et al.Development of Bacillus subtilis MP and effective utilization on productivity and microorganisms in feces of suckling piglets[J].Journal of Applied Research in Veterinary Medicine,2009,9(4):382-387.

[37] 曹廷富.微生态制剂对生长猪生产指标和粪中微生物影响的研究[D].硕士学位论文.长沙:湖南农业大学,2011.

[38] 高环,罗彬,黄兴国.微生态制剂对生长猪生产性能、养分消化率及粪中微生物的影响[J].中国饲料,2015(15):15-18.

[39] LEE S H,INGALE S L,KIM J S,et al.Effects of dietary supplementation with Bacillus subtilis LS 1-2 fermentation biomass on growth performance,nutrient digestibility,cecal microbiota and intestinal morphology of weanling pig[J].Animal Feed Science and Technology,2014,188:102-110.
Outlines

/