Effects of Protease on Standard Ileum Amino Acid Digestibility of Rapeseed Meal in Meat Ducks

ZHANG Kaixin, ZHANG Yang, ZHANG Keying, DING Xuemei, BAI Shiping, WANG Jianping, XUAN Yue, SU Zhuowei, ZENG Qiufeng

doi:10.3969/j.issn.1006-267x.2019.07.041

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 7: 3285 - 3293

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.07.041

Molecular Nutrition

Effects of Protease on Standard Ileum Amino Acid Digestibility of Rapeseed Meal in Meat Ducks

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1. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China;
2. Sichuan Mianyang Habio Bioengineering Co., Ltd., Mianyang 621000, China

Received date: 2018-12-27

Online published: 2019-07-19

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Abstract

This study was conducted to investigate the effects of protease on standard ileum amino acid digestibility of rapeseed meal in meat ducks. The metabolic experiment was using a substitution method, selected five rapeseed meals from different sources, and the rapeseed meal replacement ratio was 15%. A total of 640 one-day-old male ducks were randomly divided into 13 treatments, 12 treatments (6 replicates per treatment, 8 ducks per replicate) included 6 treatments which were the basal diet and 5 kinds of rapeseed meals replace basal diets with no protease addition, and other 6 treatments were corresponding protease addition treatments (adding 200 mg/kg protease), and another set up a nitrogen-free diet treatment (8 replicates, 8 ducks per replicate). Meat ducks were fed experimental diets from 15 to 17 days of age, and the experiment lasted for 3 days. The results showed as follows:1) the average contents of ether extract (EE), crude protein (CP), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), glucosinolates (GS), isothiocyanates (ITC) and oxazolidine thione (OZT) in 5 kinds of rapeseed meal were 8.21%, 36.22%, 12.96%, 38.65%, 13.83%, 29.89 μmol/g, 2.74 mg/g and 0.45 mg/g, respectively. The total amino acid contents of 5 kinds of rapeseed meals were 32.97%, 37.77%, 28.88%, 36.08% and 36.29% respectively, and its coefficient of variation (CV) was 10.31%; the average contents of lysine (Lys), methionine (Met) and threonine (Thr) in rapeseed meal were 1.95%, 0.47% and 1.89%, respectively, and their CV were 30.49%, 23.69% and 30.65%, respectively. 2) Different kinds of rapeseed meals had significant effects on standard ileum amino acid digestibility of meat ducks (P<0.05), and the average standard ileal digestibility of Met, Lys and Thr was 91.04%, 66.19% and 73.54%, respectively. 3) Adding protease significantly improved the standard ileal digestibility of essential amino acids such as Thr (6.73%), isoleucine (4.47%), leucine (3.03%), valine (4.60%), and arginine (2.36%) of rapeseed meal (P<0.05), as well as non-essential amino acids such as aspartic acid (8.46%), serine (7.05%), glutamate (5.97%), glycine (9.38%), alanine (8.44%) and proline (3.94%) of rapeseed meal (P<0.05). In conclusion, the standard ileum amino acid digestibility of different sources of rapeseed meal in meat ducks present a wide range, and the Met standard ileum digestibility in rapeseed meal is the highest; the improvement degree of protease improve the standard ileum amino acid digestibility of rapeseed meal in meat ducks relate to the type of amino acid.

Key words： rapeseed meal; protease; standard ileum amino acid digestibility; meat ducks

Cite this article

ZHANG Kaixin, ZHANG Yang, ZHANG Keying, DING Xuemei, BAI Shiping, WANG Jianping, XUAN Yue, SU Zhuowei, ZENG Qiufeng . Effects of Protease on Standard Ileum Amino Acid Digestibility of Rapeseed Meal in Meat Ducks[J]. Chinese Journal of Animal Nutrition, 2019 , 31(7) : 3285 -3293 . DOI: 10.3969/j.issn.1006-267x.2019.07.041

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