Molecular Nutrition

Effects of Three Species of Live Food on Growth Performance, Digestive Enzyme Activities and Immunity of Whitmania pigra Larvae

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  • 1. Agricultural Science Institute of Jiangsu Lixiahe District, Yangzhou 225007, China;
    2. Research Center for Eco-Agricultural Engineering and Technology of Jiangsu Province, Yangzhou 225009, China

Received date: 2018-12-11

  Online published: 2019-07-19

Abstract

The effects of three species of live food on the growth performance, digestive enzyme activities and immunity of Whitmania pigra larvae were analyzed by experimental ecology method in this experiment. A total of 540 Whitmania pigra larvae with an average body weight of (0.027±0.010) g were randomly divided into 3 groups with 3 replicates in each group and 60 larvae in each replicate. The snail (control), screw and earthworm were used as live food. The results showed that the body weight, body length and body width of larvae in screw group were significantly higher than those in the other two groups (P<0.05). There was no significant difference in survival rate among three groups (P>0.05). The overall growth performance of larvae from high to low was screw group, snail group and earthworm group. The correlation between growth indexes and body weight of larvae from large to small was volume (R2=0.912 0), body length (R2=0.888 8) and body width (R2=0.771 7). There was no significant difference in protease activity among snail group, screw group and earthworm group (P>0.05). The amylase activity of larval leech in snail group was significantly higher than that in screw group and earthworm group (P<0.05). The lipase activity of larval leech in screw group was significantly higher than that in snail group and earthworm group (P<0.05). There was no significant difference in superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) among snail group, screw group and earthworm group (P>0.05). The alkaline phosphatase (AKP) and acid phosphatase (ACP) activities of larval leech in snail group were significantly higher than those in screw group and earthworm group (P<0.05). The catalase (CAT) and lysozyme (LZM) activities of larval leech in earthworm group were significantly higher than those in snail group and screw group (P<0.05), and there was no significant difference between snail group and screw group (P>0.05). Considered the growth and digestive enzyme activities, the screw is the best live food which can make leech larval acquire the best growth ability. Comprehensive analysis, the growth-promoting effect of screw is best for Whitmania pigra larvae. It is suggested that the nutritive standard of artificial feed for larval leech can be established according to the nutritive composition of screw, and earthworm can be used as the immune additive of artificial feed.

Cite this article

WU Leiming, HAN Guangming, KOU Xiangming, WANG Shouhong, ZHANG Jiahong, BI Jianhua, WANG Guiliang, TANG Hejun, XU Rong, ZHU Lingyu . Effects of Three Species of Live Food on Growth Performance, Digestive Enzyme Activities and Immunity of Whitmania pigra Larvae[J]. Chinese Journal of Animal Nutrition, 2019 , 31(7) : 3383 -3390 . DOI: 10.3969/j.issn.1006-267x.2019.07.052

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