Differences of Bacterial Structure and Composition in Rumen of Goats with Different Nitrogen Utilization

  • LI Qiong ,
  • WANG Lizhi ,
  • WANG Zhisheng ,
  • XUE Bai ,
  • PENG Quanhui
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  • Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2019-02-18

  Online published: 2019-08-19

Abstract

The objective of this study was to compare the differences in the bacterial structure and composition in rumen of goats with different nitrogen utilization. Thirty goats were chosen as the experimental animals, according to the data of metabolic test, goats were chosen as the high nitrogen utilization (HNU) group and low nitrogen utilization (LNU) group. Rumen contents of each goat in 2 groups were collected after slaughter and the differences of their bacterial structure and composition were analyzed and compared by high-throughput sequencing technique, and 7 proteolytic bacteria were quantitatively analyzed and compared by real-time PCR. The results showed as follows:1) there were obvious individual differences in the nitrogen utilization in 30 goats, the average nitrogen utilization of HNU group and LNU group was (56.68±5.54)% and (34.25±4.21)%, and there was significant difference between groups (P<0.01). 2) The differences of rumen microorganisms structure in HNU group and LNU group were different at the genus level. The relative abundances of Prevotella 1, Succiniclasticum, p-1088-a5_gut_group in HNU group were significantly lower than those in LNU group (P<0.05), and the relative abundances of Lachnospiraceae_XPB_1014_group, Ruminococcus 1, Ruminococcaceae_UCG_004, Ruminobacter, Family_ⅩⅢ_UCG_002 and Anaeroplasma groups were significantly higher than those in LNU group (P<0.05). 3) The further analysis showed that the relative abundance of Ruminococcus 1, Ruminobacter and Anaeroplasma had a positive relationship with the host nitrogen utilization (P<0.05). 4) The numbers of Butyrivibrio fibrisolvens, Fibrobacter succinogenes and Ruminococcus_sp._HUN007 in HNU group were significantly higher than those in LNU group (P<0.05), and the numbers of Prevotella ruminicola, Streptococcus bovis, Succinivibrio dextrinisolvens and Ruminococcus flavefaciens had no differences between two groups (P>0.05). In summary, there are significant differences on the bacterial structure and composition in rumen of goats with different nitrogen utilization.

Cite this article

LI Qiong , WANG Lizhi , WANG Zhisheng , XUE Bai , PENG Quanhui . Differences of Bacterial Structure and Composition in Rumen of Goats with Different Nitrogen Utilization[J]. Chinese Journal of Animal Nutrition, 2019 , 31(8) : 3651 -3663 . DOI: 10.3969/j.issn.1006-267x.2019.08.026

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