Effects of Glutamine Dipeptide On Growth, Serum Biochemistry, Immunity Indexes and Intestinal Morphology of Juvenile Grass Carp (Ctenopharyngodon idellus)

WU Tongqiang; ZHONG Lei; LIU Zhuangpeng; HU Yi; LIU Zhen; LU Shuangqing

doi:10.3969/j.issn.1006-267x.2019.08.029

2019 , Vol. 31 >Issue 8: 3682 - 3689

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.029

Effects of Glutamine Dipeptide On Growth, Serum Biochemistry, Immunity Indexes and Intestinal Morphology of Juvenile Grass Carp (Ctenopharyngodon idellus)

WU Tongqiang ,
ZHONG Lei ,
LIU Zhuangpeng ,
HU Yi ,
LIU Zhen ,
LU Shuangqing

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1. College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China;
2. Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China;
3. Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Department of Biological and Environmental Engineering, Changsha University, Changsha 410022, China

Received date: 2019-01-21

Online published: 2019-08-19

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Abstract

The aim of this experiment was to study the effects of glutamine dipeptide (Gly-Gln) on the growth, serum biochemistry, immunity indexes and intestinal morphology of juvenile grass carp. A total of 750 fish with an average body weight of (7.16±0.10) g were selected and divided into 5 groups with 3 replicates per group and 50 fish per replicate. Fish were fed diets containing 0 (control group) and 0.25%, 0.50%, 0.75% and 1.00% glutamine dipeptide, respectively. The feeding test lasted for 8 weeks. The results showed as follows:1) compared with the control group, the weight gain rate and protein efficiency of juvenile grass carp increased significantly (P<0.05) and the feed coefficient decreased significantly (P<0.05) in the glutamine dipeptide supplementation groups. Dietary supplementation of 0.25% and 0.50% glutamine dipeptide significantly increased intestinal lipase and trypsin activities (P<0.05). 2) Compared with the control group, the serum glucose content in 0.75% group significantly increased (P<0.05), and the serum urea nitrogen content in each glutamine dipeptide supplementation group significantly increased (P<0.05). The addition of glutamine dipeptide in the feed made the serum triglyceride and cholesterol contents decrease, and the cholesterol content in 0.50% group significantly decreased compared with the control group (P<0.05), serum triglyceride content in each supplementation group significantly decreased compared with the control group (P<0.05). The activities of glutamic oxalacetic transaminase and alanine aminotransferase significantly increased when the dosage was more than 0.50% (P<0.05). 3) Compared with the control group, the serum lysozyme activity in 0.50% group increased significantly (P<0.05). Serum complement 4 content decreased significantly in 0.75% and 1.00% groups (P<0.05), while the cortisol content increased significantly (P<0.05). The serum glutathione content and total antioxidant capacity significantly increased in each supplementation group (P<0.05). 4) Compared with the control group, the intestinal crypt depth significantly reduced in 0.5% group (P<0.05), the intestinal mucosal thickness significantly increased in the 0.75% and 1.00% groups (P<0.05), and the number of goblet cells significantly increased in the 0.50% and 1.00% groups (P<0.05). In summary, the addition of 0.50% glutamine dipeptide to the feed can promote nutrient metabolism, immunity and intestinal development of grass carp juveniles, thereby promoting growth.

Key words： glutamine dipeptide; juvenile grass carp; growth performance; immunity; intestinal morphology

Cite this article

WU Tongqiang , ZHONG Lei , LIU Zhuangpeng , HU Yi , LIU Zhen , LU Shuangqing . Effects of Glutamine Dipeptide On Growth, Serum Biochemistry, Immunity Indexes and Intestinal Morphology of Juvenile Grass Carp (Ctenopharyngodon idellus)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(8) : 3682 -3689 . DOI: 10.3969/j.issn.1006-267x.2019.08.029

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