Threonine Promotes Gut Mucosal Mucin Expressions in Weaned Piglets via Regulating Protein Kinase B/Mammalian Target of Rapamycin Signaling Pathway

MAO Xiangbing; CHEN Daiwen; YU Bing; HE Jun; YU Jie; ZHENG Ping; LUO Junqiu; LUO Yuheng; WANG Quyuan; WANG Huifen

doi:10.3969/j.issn.1006-267x.2019.08.031

2019 , Vol. 31 >Issue 8: 3699 - 3705

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.031

Threonine Promotes Gut Mucosal Mucin Expressions in Weaned Piglets via Regulating Protein Kinase B/Mammalian Target of Rapamycin Signaling Pathway

MAO Xiangbing ,
CHEN Daiwen ,
YU Bing ,
HE Jun ,
YU Jie ,
ZHENG Ping ,
LUO Junqiu ,
LUO Yuheng ,
WANG Quyuan ,
WANG Huifen

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Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2019-02-03

Online published: 2019-08-19

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Abstract

This experiment was conducted to determine the possible mechanism of dietary threonine supplementation increasing mucin expressions in gut mucosa. Ten healthy crossbred (Duroc×Landrace×Yorkshire) piglets weaned at 21 days of age were randomly allotted into 2 groups (n=5) and fed diets contained 0.74% (the basal diet) and 0.89% (the basal diet supplemented threonine) true ileal digestible threonine. The trial duration was 21 days. The results showed that dietary threonine supplementation did not significantly affect average daily gain and average daily feed intake (P>0.05), significantly reduced feed/gain (P<0.05), significantly increased the contents and mRNA relative expression levels of mucin 1 and 2 in jejunal and ileal mucosa (P<0.05), and significantly stimulated the phosphorylation of protein kinase B, mammalian target of rapamycin, ribosomal protein S6 kinase and eukaryotic translation initiation factor 4E-binding protein 1 in jejunal and ileal mucosa of weaned piglets (P<0.05). These results suggest that dietary threonine supplementation (0.89% true ileal digestible threonine) promotes the mucin production of gut mucosa in weaned piglets, which may be derived from both the transcription of genes and the activation of protein kinase B/mammalian target of rapamycin signaling pathway.

Key words： threonine; weaned piglets; gut mucins; Akt/mTOR signaling pathway

Cite this article

MAO Xiangbing , CHEN Daiwen , YU Bing , HE Jun , YU Jie , ZHENG Ping , LUO Junqiu , LUO Yuheng , WANG Quyuan , WANG Huifen . Threonine Promotes Gut Mucosal Mucin Expressions in Weaned Piglets via Regulating Protein Kinase B/Mammalian Target of Rapamycin Signaling Pathway[J]. Chinese Journal of Animal Nutrition, 2019 , 31(8) : 3699 -3705 . DOI: 10.3969/j.issn.1006-267x.2019.08.031

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