Effects of Lactobacillus paracasei-Fermented Feed on Growth Performance, Fecal Bacteria Populations and Volatile Fatty Acid Contents, and Serum Biochemical and Immune Indexes of Growing Pigs

  • LIU Hui ,
  • JI Haifeng ,
  • WANG Sixin ,
  • ZHANG Dongyan ,
  • WANG Jing ,
  • ZHANG Wei ,
  • WANG Yamin
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  • Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received date: 2019-01-16

  Online published: 2019-08-19

Abstract

This experiment was conducted to evaluate the effects of Lactobacillus paracasei-fermented feed on growth performance, fecal bacteria populations and volatile fatty acid contents, and serum biochemical and immune indexes of growing pigs. A total of 140 Landrace×Yorkshire crossbred growing pigs with an average body weight of (20.86±0.62) kg were divided into 2 groups:control group and experimental group. Each group had 5 replicates with 14 pigs per replicate. Pigs in the control group were fed a basal diet, and pigs in experimental group were fed an experimental diet which consisted of basal diet (95%) and Lactobacillus paracasei-fermented feed (5%). The pre-experimental period was 5 days, and the experimental period was 31 days. The results showed that, compared with the control group:1) the final weight and average daily gain were increased by 6.32% (P<0.05) and 12.23% (P<0.05), respectively, and the feed/gain was decreased by 8.58% (P<0.05) in the experimental group; 2) the population of fecal lactic acid bacteria was significantly increased (P<0.05), and the populations of Escherichia coli and Staphylococcus aureus were significantly decreased (P<0.05) in the experimental group; 3) the contents of acetic acid, butyric acid and total volatile fatty acid in feces in the experimental group were increased by 13.44% (P<0.05), 20.51% (P<0.05) and 11.01% (P<0.05), respectively; 4) the contents of serum total protein, globulin, immunoglobulin G and immunoglobulin A were increased by 18.85% (P<0.05), 33.31% (P<0.05), 15.85% (P<0.05) and 45.86% (P<0.05), and the contents of serum urea nitrogen and haptoglobin were decreased by 19.29% (P<0.05) and 52.72% (P<0.05) in the experimental group, respectively. The results indicate that Lactobacillus paracasei-fermented feed can improve the growth performance, regulate the intestinal microbial balance, enhance the immune function and increase the contents of volatile fatty acids in feces of growing pigs.

Cite this article

LIU Hui , JI Haifeng , WANG Sixin , ZHANG Dongyan , WANG Jing , ZHANG Wei , WANG Yamin . Effects of Lactobacillus paracasei-Fermented Feed on Growth Performance, Fecal Bacteria Populations and Volatile Fatty Acid Contents, and Serum Biochemical and Immune Indexes of Growing Pigs[J]. Chinese Journal of Animal Nutrition, 2019 , 31(8) : 3747 -3754 . DOI: 10.3969/j.issn.1006-267x.2019.08.036

References

[1] 李永凯,毛胜勇,朱伟云.益生菌发酵饲料研究及应用现状[J].畜牧与兽医,2009,41(3):90-93.
[2] 李慧芬,曾亚均.乳酸菌在固态发酵饲料中的应用[J].中国饲料,2018(17):86-89.
[3] CANIBE N,HØJBERG O,BADSBERG J H,et al.Effect of feeding fermented liquid feed and fermented grain on gastrointestinal ecology and growth performance in piglets[J].Journal of Animal Science,2007,85(11):2959-2971.  
[4] MISSOTTEN J A M,MICHIELS J,DEGROOTE J,et al.Fermented liquid feed for pigs:an ancient technique for the future[J].Journal of Animal Science and Biotechnology,2015,6(1):4.
[5] 张秀林,魏小兵,欧长波,等.益生菌发酵饲料对仔猪生长和免疫功能影响的研究进展[J].中国畜牧兽医,2017,44(2):476-481.
[6] 魏爱彬,于洁,王鑫,等.益生乳酸菌Lactobacillus casei Zhang和Lactobacillus plantarum P8对全价饲料pH及微生物类群变化的研究[J].微生物学杂志,2012,32(2):1-8.
[7] 涂小丽,钟芳,邓伏清,等.微生物发酵饲料对生长猪生产性能和营养物质利用率的影响[J].饲料工业,2015,36(4):36-38.
[8] WANG C,SHI C Y,ZHANG Y,et al.Microbiota in fermented feed and swine gut[J].Applied Microbiology and Biotechnology,2018,102(7):2941-2948.  
[9] HU J K,LU W Q,WANG C L,et al.Characteristics of solid-state fermented feed and its effects on performance and nutrient digestibility in growing-finishing pigs[J].Asian-Australasian Journal of Animal Sciences,2018,21(11):1635-1641.
[10] CANANI R B,CORSELLO G,NOCERINO R,et al.Effect of fermented milk with Lactobacillus paracasei CBA L74 on gastrointestinal and respiratory infections in children:multicenter randomized controlled trial[J].Digestive and Liver Disease,2016,48(Suppl.4):e280-e281.
[11] FRANKO B,VAILLANT M,RECULE C,et al.Lactobacillus paracasei endocarditis in a consumer of probiotics[J].Médecine et Maladies Infectieuses,2013,43(4):171-173.  
[12] QIAO J Y,LI H H,WANG Z X,et al.Effects of Lactobacillus acidophilus dietary supplementation on the performance,intestinal barrier function,rectal microflora and serum immune function in weaned piglets challenged with Escherichia coli lipopolysaccharide[J].Antonie van Leeuwenhoek,2015,107(4):883-891.  
[13] CANIBE N,JENSEN B B.Fermented and nonfermented liquid feed to growing pigs:effect on aspects of gastrointestinal ecology and growth performance[J].Journal of Animal Science,2003,81(8):2019-2031.  
[14] 王志祥,乔家运,王自恒,等.乳酸杆菌对断奶仔猪生长性能、养分表观消化率和消化酶活性的影响[J].西北农林科技大学学报(自然科学版),2006,34(4):23-27.
[15] 何谦,吴同山,胡文锋,等.发酵饲料对规模化猪场断奶仔猪生产性能的影响[J].畜牧与兽医,2008,40(6):62-64.
[16] 张铮,石青松,朱伟云,等.乳酸菌发酵饲料对断奶仔猪生长性能和肠道健康的影响[J].江苏农业科学,2018,46(19):170-173.
[17] RUSSELL P J,GEARY T M,BROOKS P H,et al.Performance,water use and effluent output of weaner pigs fed ad libitum with either dry pellets or liquid feed and the role of microbial activity in the liquid feed[J].Journal of the Science of Food and Agriculture,1996,72(1):8-16.  
[18] JENSEN B B,MIKKELSEN L L.Feeding liquid diets to pigs[M]//GARNSWORTHY P C,WISEMAN J.Recent advance in animal nutrition.Nottingham:Nottingham University Press,1998:107-126.
[19] LESCHEID D W.Probiotics as regulators of inflammation:a review[J].Functional Foods in Health and Disease,2014,4(7):299-311.  
[20] VAN WINSEN R L,KEUZENKAMP D,URLINGS B A P,et al.Effect of fermented feed on shedding of Enterobacteriaceae by fattening pigs[J].Veterinary Microbiology,2002,87(3):267-276.  
[21] 刘金萍.植物乳杆菌A6(Lactobacillus plantarum A6)生物学特性及在发酵饲料中应用的研究[D].硕士学位论文.南宁:广西大学,2004.
[22] YIN F G,FARZAN A,WANG Q,et al.Reduction of Salmonella enterica serovar typhimurium DT104 infection in experimentally challenged weaned pigs fed a Lactobacillus-fermented feed[J].Foodborne Pathogens and Disease,2014,11(8):628-634.  
[23] VAN WINSEN R L,URLINGS B A P,LIPMAN L J A,et al.Effect of fermented feed on the microbial population of the gastrointestinal tracts of pigs[J].Applied and Environmental Microbiology,2001,67(7):3071-3076.  
[24] 陈鲜鑫,王金全,王春阳,等.乳酸菌发酵液体饲料对生长猪生长性能和粪中微生物区系的影响[J].饲料工业,2010,31(4):40-42.
[25] HÖGBERG A,LINDBERG J E.The effect of level and type of cereal non-starch polysaccharides on the performance,nutrient utilization and gut environment of pigs around weaning[J].Animal Feed Science and Technology,2006,127(3/4):200-219.
[26] LOH T C,VAN THU T,FOO H L,et al.Effects of different levels of metabolite combination produced by Lactobacillus plantarum on growth performance,diarrhoea,gut environment and digestibility of postweaning piglets[J].Journal of Applied Animal Research,2013,41(2):200-207.  
[27] MISSOTTEN J A M,MICHIELS J,OVYN A,et al.Fermented liquid feed for pigs[J].Archives of Animal Nutrition,2010,64(6):437-466.  
[28] LIU X T,HOU C L,ZHANG J,et al.Fermentation conditions influence the fatty acid composition of the membranes of Lactobacillus reuteri I5007 and its survival following freeze-drying[J].Letters in Applied Microbiology,2014,59(4):398-403.  
[29] GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science,2010,129(1/2/3):95-103.
[30] 金三俊,董佳琦,任红立,等.复合微生态制剂对断奶仔猪生长性能、血清生化和免疫指标及粪便中挥发性脂肪酸含量的影响[J].动物营养学报,2017,29(12):4477-4484.
[31] 斯文森.家畜生理学[M].华北农业大学,译.北京:科学出版社,1978.
[32] 崔艳红,韩庆功,崔艺佳,等.益生菌复合发酵料对断奶仔猪消化环境、血清生化指标和代谢激素水平的影响[J].西北农业学报,2018,27(1):16-23.
[33] 张董燕,季海峰,王晶,等.猪源罗伊氏乳酸杆菌对断奶仔猪生长性能和血清指标的影响[J].动物营养学报,2011,23(9):1553-1559.
[34] 刘辉,季海峰,张董燕,等.饲粮添加短乳杆菌对生长猪生长性能和血清生化指标的影响[J].动物营养学报,2013,25(1):182-189.
[35] MALMOLF K.Amino acid in farm animal nutrition metabolism,partition and consequences of imbalance[J].Swedish Journal of Agricultural Research,1988,18(4):191-193.
[36] BORG B S,LIBAL G W,WAHLSTROM R C.Tryptophan and threonine requirements of young pigs and their effects on serum calcium,phosphorus and zinc concentrations[J].Journal of Animal Science,1987,64(4):1070-1078.  
[37] 胡新旭,周映华,卞巧,等.无抗发酵饲料对生长育肥猪生产性能、血液生化指标和肉品质的影响[J].华中农业大学学报,2015,34(1):72-77.
[38] PETERSEN H H,NIELSEN J P,HEEGAARD P M.Application of acute phase protein measurements in veterinary clinical chemistry[J].Veterinary Research,2004,35(2):163-187.  
[39] FRANCISCO C J,BANE D P,WEIGEL R M,et al.The influence of pen density,weaning age,and feeder space on serum haptoglobin concentration in young growing swine[J].Journal of Swine Health and Production,1996,4(2):67-71.
[40] AI W Q,YUE Y,XIONG S D,et al.Enhanced protection against pulmonary mycobacterial challenge by chitosan-formulated polyepitope gene vaccine is associated with increased pulmonary secretory IgA and gamma-interferon+ T cell responses[J].Microbiology and Immunology,2013,57(3):224-235.  
[41] KUNAVUE N,LIEN T F.Effects of fulvic acid and probiotic on growth performance,nutrient digestibility,blood parameters and immunity of pigs[J].Journal of Animal Science Advances,2012,2(8):711-721.
[42] MIZUMACHI K,AOKI R,OHMORI H,et al.Effect of fermented liquid diet prepared with Lactobacillus plantarum LQ80 on the immune response in weaning pigs[J].Animal,2009,3(5):670-676.  
[43] 王娟娟,王顺喜,陆文清,等.无抗生素微生物发酵饲料对仔猪免疫及抗氧化功能的影响[J].中国饲料,2011(16):25-27,30.
[44] SÁNCHEZ B,GUEIMONDE M,PEÑA A S,et al.Intestinal microbiota as modulators of the immune system[J].Journal of Immunology Research,2015,2015:159094.
[45] GILL H S.Stimulation of the immune system by lactic cultures[J].International Dairy Journal,1998,8(5/6):535-544.
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