Effects of Supplemented with Different Levels of Equol on Growth Performance, Plasma Equol Content and Reproductive Hormone Levels and Antioxidant Capacity of Female Sheep

WANG Chenchen; ZHANG Wenjie; HOU Chuanxiang; LI Xiaobin; ZHAO Guodong; YANG Kailun

doi:10.3969/j.issn.1006-267x.2019.08.042

2019 , Vol. 31 >Issue 8: 3801 - 3809

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.042

Effects of Supplemented with Different Levels of Equol on Growth Performance, Plasma Equol Content and Reproductive Hormone Levels and Antioxidant Capacity of Female Sheep

WANG Chenchen ,
ZHANG Wenjie ,
HOU Chuanxiang ,
LI Xiaobin ,
ZHAO Guodong ,
YANG Kailun

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Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2019-01-21

Online published: 2019-08-19

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Abstract

This experiment was conducted to investigate the effects of supplemented with different levels of equol on growth performance, plasma equol content and reproductive hormone levels and antioxidant capacity of female sheep. Twenty Kazakh female sheep with an average body weight of (36.73±2.42) kg, aged 2 years old, were randomly divided into 4 groups (5 sheep in each group), being control group, trial group Ⅰ, trial group Ⅱ and trial group Ⅲ. Each sheep in all group was fed under the same feeding condition, additionally, sheep in trial group Ⅰ, trial group Ⅱ and trial group Ⅲ were fed with 10, 25 and 50 mg equol at 08:00 every day per sheep, respectively. The supplementary feeding experiment lasted for 14 days. The results showed as follows:1) compared with control group, supplementation of 50 mg/(d·sheep) equol can significantly improve the average daily gain and average daily feed intake (P<0.05 or P<0.01), while reduce the feed/gain (P<0.05). 2) After different dosages of equol were supplementary fed, the plasma equol content tendency rising up at the beginning and declining in late, and it peaked around 2.0 to 3.0 h. 3) The plasma testosterone (T) level in trial group Ⅰ was significantly higher than that in control group (P<0.01) and trial group Ⅲ (P<0.05), and that in trial group Ⅱ was also significantly higher than that in control group (P<0.05), but there was no significant difference between trial group Ⅰ and trial group Ⅲ (P>0.05). The plasma dihydrotestosterone (DHT) level in trial group Ⅰ and trial group Ⅱ was significantly higher than that in control group and trial group Ⅲ (P<0.05). 4) The plasma total antioxidant capacity (T-AOC) and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in trial group Ⅱ had the highest values, while the malondialdehyde (MDA) content had the lowest value, and the differences in above indices were significant compared with control group (P<0.05 or P<0.01). Therefore, supplementation of 50 mg/(d·sheep) equol can significantly improve the growth performance of sheep; plasma equol content reaches its peak at 2.0 to 3.0 h after supplementary feeding equol, and with the supplemental dosage of equol increasing, the high equol content (i.e. duration time of high plasma equol content) prolongs in cross-peak time, too. Supplementation of 10 and 25 mg/(d·sheep) equol can significantly elevate the plasma T and DHT levels of female sheep. Supplementation of 25 mg/(d·sheep) equol can significantly enhance the antioxidant capacity of female sheep, and the effect is better than 10 and 50 mg/(d·sheep).

Key words： sheep; equol; growth performance; reproductive hormones; antioxidant capacity

Cite this article

WANG Chenchen , ZHANG Wenjie , HOU Chuanxiang , LI Xiaobin , ZHAO Guodong , YANG Kailun . Effects of Supplemented with Different Levels of Equol on Growth Performance, Plasma Equol Content and Reproductive Hormone Levels and Antioxidant Capacity of Female Sheep[J]. Chinese Journal of Animal Nutrition, 2019 , 31(8) : 3801 -3809 . DOI: 10.3969/j.issn.1006-267x.2019.08.042

References

[1] 郑庆虎,周辉,陈祯祥,等.雌马酚的生物学作用及其在医学领域的研究进展[J].中国皮肤性病学杂志,2018(1):105-109.
[2] SETCHELL K D R,BROWN N M,LYDEKING-OLSEN E.The clinical importance of the metabolite equol-a clue to the effectiveness of soy and its isoflavones[J].The Journal of Nutrition,2002,132(12):3577-3584.

[3] SHUTT D A,COX R I.Steroid and phyto-oestrogen binding to sheep uterine receptors in vitro[J].Journal of Endocrinology,1972,52(2):299-310.

[4] SETCHELL K D R,CLERICI C,LEPHART E D,et al.S-equol,a potent ligand for estrogen receptor β,is the exclusive enantiomeric form of the soy isoflavone metabolite produced by human intestinal bacterial flora[J].The American Journal of Clinical Nutrition,2005,81(5):1072-1079.

[5] LEGETTE L L,MARTIN B R,SHAHNAZARI M,et al.Supplemental dietary racemic equol has modest benefits to bone but has mild uterotropic activity in ovariectomized rats[J].The Journal of Nutrition,2009,139(10):1908-1913.

[6] ARORA A,NAIR M G,STRASBURG G M.Antioxidant activities of isoflavones and their biological metabolites in a liposomal system[J].Archives of Biochemistry and Biophysics,1998,356(2):133-141.

[7] 马得莹,单安山,杜娟.大豆黄酮对动物生产性能、免疫功能与抗氧化作用的影响[J].东北农业大学学报,2004,35(4):505-508.
[8] 林厦菁,蒋守群,林哲敏,等.大豆异黄酮和抗生素对文昌鸡生长性能、肉品质和血浆抗氧化指标的影响[J].华南农业大学学报,2018,39(1):1-6.
[9] 周玲玲,徐建雄.高活性大豆异黄酮发酵豆粕对断奶仔猪生长性能、抗氧化能力、肝功能指标的影响[J].饲料工业,2017,38(20):34-38.
[10] JÖEHLE W,LAMOND D R.Control of reproductive functions in domestic animals[M].London:Martinus Niihoff,1980,28N49.
[11] FORBES J M.The effects of sex hormones,pregnancy and lactation on digestion,metabolism and voluntary food intake[M]//MILLIGAN L P,GROVUM W L,DOBSON A.Control of digestion and metabolism in ruminants.New Jersey:Prentice-Hall,1986.
[12] 李海亮,邓颖,王欣,等.肠道微生物代谢产物——S-雌马酚与人类健康关系研究进展[J].中国微生态学杂志,2018,30(3):362-367.
[13] SETCHELL K D R,ZHAO X H,JHA P,et al.The pharmacokinetic behavior of the soy isoflavone metabolite S-(-)equol and its diastereoisomer R-(+)equol in healthy adults determined by using stable-isotope-labeled tracers[J].The American Journal of Clinical Nutrition,2009,90(4):1029-1037.

[14] 王洋,张玉梅.雌马酚人体代谢的研究进展[J].卫生研究,2010,39(6):787-789.
[15] CHOI E J.Evaluation of equol function on anti-or prooxidant status in vivo[J].Journal of Food Science,2009,74(4):64-71.
[16] WALSH K R,FAILLA M L.Transport and metabolism of equol by Caco-2 human intestinal cells[J].Journal of Agricultural and Food Chemistry,2009,57(18):8297-8302.

[17] SETCHELL K D R,ZHAO X H,SHOAF S E,et al.The pharmacokinetics of S-(-)equol administered as SE5-OH tablets to healthy postmenopausal women[J].The Journal of Nutrition,2009,139(11):2037-2043.

[18] SFAKIANOS J,COWARD L,KIRK M,et al.Intestinal uptake and biliary excretion of the isoflavone genistein in rats[J].The Journal of Nutrition,1997,127(7):1260-1268.

[19] MORITO K,HIROSE T,KINJO J,et al.Interaction of phytoestrogens with estrogen receptors α and β[J].Biological and Pharmaceutical Bulletin,2001,24(4):351-356.

[20] LUND T D,MUNSON D J,HALDY M E,et al.Equol is a novel anti-androgen that inhibits prostate growth and hormone feedback[J].Biology of Reproduction,2004,70(4):1188-1195.

[21] 张晓娜,杨镒峰,常彤,等.雌激素、雄激素在卵泡发育中的作用及应用[J].特产研究,2017,39(3):50-54.
[22] 王镜岩.生物化学(上册)[M].3版.北京:高等教育出版社,2002:556-583.
[23] 吴彬,张勇,陈明亮,等.雌马酚对去卵巢后大鼠骨质疏松症的影响[J].第三军医大学学报,2015,37(3):256-260.
[24] 李琮,陈俊宏,刘亚伟,等.大豆黄酮、芒柄花素及其组合对奶牛产奶性能、血浆和乳中激素含量的影响[J].动物营养学报,2017,29(1):309-316.
[25] ADLERCREUTZ H,FOTSIS T,BANNWART C,et al.Determination of urinary lignans and phytoestrogen metabolites, potential antiestrogens and anticarcinogens, in urine of women on various habitual diets[J].Journal of Steroid Biochemistry,1986,25(5):791-797.

[26] BELL A W.Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation[J].Journal of Animal Science,1995,73(9):2804-2819.

[27] DIBNER J J,KITCHELL M L,ATWELL C A,et al.The effect of dietary ingredients and age on the microscopic structure of the gastrointestinal tract in poultry[J].The Journal of Applied Poultry Research,1996(5):70-77.
[28] KLADNA A,BERCZYNSKI P,KRUK I,et al.Studies on the antioxidant properties of some phytoestrogens[J].Luminescence,2016,31(6):1201-1206.

[29] 李琮,臧长江,刘亚伟,等.添喂两种植物雌激素对奶牛乳成分、血液生化指标和抗氧化性能的影响[J].中国畜牧兽医,2017(6):126-130.
[30] HODGSON J M,CROFT K D,PUDDEY I B,et al.Soybean isoflavonoids and their metabolic products inhibit in vitro lipoprotein oxidation in serum[J].The Journal of Nutritional Biochemistry,1996,7(12):664-669.

[31] MITCHELL J H,GARDNER P T,MCPHAIL D B,et al.Antioxidant efficacy of phytoestrogens in chemical and biological model systems[J].Archives of Biochemistry and Biophysics,1998,360(1):142-148.

[32] CHOI E J.evaluation of equol function on anti-or prooxidant status in vivo[J].Journal of Food Science,2009,74(2):H65-H71.
[33] 苟钟勇,蒋守群,郑春田,等.雌马酚对鸡巨噬细胞抗氧化保护和免疫调控作用的研究[C]//全国动物生理生化第七届全国代表大会暨第十三次学术交流会论文集.沈阳:中国畜牧兽医学会,2014:193.
[34] 林厦菁,蒋守群.雌马酚对鸡小肠上皮细胞氧化应激模型的影响[C]//全国动物生理生化第七届全国代表大会暨第十三次学术交流会论文集.沈阳:中国畜牧兽医学会,2014:184-185.

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