Study on Gallic Acid from Galla chinensis by Ultrasonic-Assisted Tannin Enzymatic Method and Its Antioxygenation

LI Zhen; LI Ping

doi:10.3969/j.issn.1006-267x.2019.08.045

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 8: 3830 - 3842

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.045

Study on Gallic Acid from Galla chinensis by Ultrasonic-Assisted Tannin Enzymatic Method and Its Antioxygenation

LI Zhen ,
LI Ping

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1. College of Pharmacy, Linyi University, Linyi 276005, China;
2. Shandong Lunan Chinese Medicinal Materials Resources Development Engineering Technology Research Center, Linyi 276005, China;
3. Linyi People's Hospital, Linyi 276003, China

Received date: 2019-01-16

Online published: 2019-08-19

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Abstract

This study was conducted to explore the optimum extraction process conditions of gallic acid from Galla chinensist by ultrasonic-assisted tannin enzymatic method and its antioxygenation. A single factor design was adopted in the experiment, to study the effects of tannase enzyme addition amount, enzymolysis temperature, enzymolysis time, pH, ultrasonic power on yield of gallic acid, and the response surface method was used to optimize the extraction process condition of gallic acid from Galla chinensis. Forty healthy Kunming mice (half male and half female) with the body weight (BW) of (20±2) g were selected and divided into 4 groups with 10 mice per group. Mice in the blank control group were gavage 10 g BW 0.1 mL normal saline, and mice in the experimental groups were gavage 50 (low dose group), 75 (medium dose group) and 100 mg/kg BW (high dose group) gallic acid solution of the same volume, respectively, one times a day, and the experiment last for 21 days. The results showed that the optimum extraction process conditions of gallic acid were as follows:the tannase enzyme addition amount was 11 U/g, the enzymolysis temperature was 45℃,the enzymolysis time was 4.8 h, the pH was 6 and the ultrasonic power was 380 W. Under the above conditions, the yield of gallic acid was (64.73±1.61)%. The purity of gallic acid was (99.01±2.47)% which puritied by crystallization method and ion exchange resin method. On the mass concentration of 60 μg/mL, the reducing force was 0.85±0.04, and the scavenging rates of 1,1-diphenyl-2-picrylhydrazine radical (DPPH·) and superoxide anion radical (O₂^-·) were (92.11±2.38)% and (98.62±2.75)%, respectively. Compared with the blank control group, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in liver of medium and high dose groups were significantly increased (P<0.05 and P<0.01), and the liver malondialdehyde (MDA) content of low, medium and high dose groups was significantly decreased (P<0.05 and P<0.01). Therefore, the ultrasonic-assisted tannin enzymatic method can improve the yield of gallic acid, the gallic acid can scavenge free radical effectively and has good antioxygenation.

Key words： Galla chinensis; gallic acid; ultrasonic; tannase enzyme; response surface method; antioxidant

Cite this article

LI Zhen , LI Ping . Study on Gallic Acid from Galla chinensis by Ultrasonic-Assisted Tannin Enzymatic Method and Its Antioxygenation[J]. Chinese Journal of Animal Nutrition, 2019 , 31(8) : 3830 -3842 . DOI: 10.3969/j.issn.1006-267x.2019.08.045

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