Effects of Short Chain Fructo-Oligosaccharide on Intestinal Redox Status and Barrier Function of Weaning Piglets

LIU Jingbo; CAO Shanchuan; YANG Yong; LIU Ming; ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2019.08.048

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 8: 3863 - 3871

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.048

Effects of Short Chain Fructo-Oligosaccharide on Intestinal Redox Status and Barrier Function of Weaning Piglets

LIU Jingbo ,
CAO Shanchuan ,
YANG Yong ,
LIU Ming ,
ZHANG Hongfu

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1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Beijing China-Agri Hongke Bio-Technology Co., Ltd., Beijing 100193, China

Received date: 2019-01-18

Online published: 2019-08-19

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Abstract

The present study was conducted to determine the effects of short chain fructo-oligosaccharide (scFOS) on intestinal redox status and mucosal integrity of weaning piglets. Twelve 24-day-old weaned piglets[Duroc×(Landrace×Large White)] with the initial body weight of (7.02±0.04) kg were randomly assigned into control group (n=6) and scFOS group (n=6). Piglets in the control group were fed a basal diet, while piglets in the scFOS group were fed the basal diet and were given a fixed amount of scFOS at 1.2 g per day. The trial lasted for 14 days. The results showed as follows:1) the scFOS intake significantly increased average daily gain and significantly decreased feed to gain ratio (P<0.05). scFOS consumption significantly decreased plasma diamine oxidase activity and D-lactic acid content (P<0.05). 2) scFOS consumption significantly increased reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) activity and significantly decreased malonaldehyde (MDA) content in jejunal mucosa of weaning piglets (P<0.05). 3) Likely, the mRNA expression of GSH-Px and nuclear transcription factor NF-E2 related factor 2 (NRF2) in jejunal mucosa significantly up-regulated in response to scFOS intake (P<0.05), and the mRNA abundance of Keleh-like epichlorohydrin associated protein 1 (KEAP1) tended to increase in scFOS-fed piglets than their counterparts (P=0.08). 4) Moreover, scFOS consumption significantly increased tight junction protein 2 (ZO2) and occludin 2 (CLDN2) mRNA expression in jejunal mucosa of weaning piglets (P<0.05). There was a trend toward higher mRNA expression of claudin (OCLD) in jejunal mucosa of scFOS-fed piglets than that of control piglets (P=0.05). Collectively, short-term scFOS consumption significantly improves growth performance of weaning piglets, which may be associated with improved intestinal redox status and mucosa barrier function.

Key words： scFOS; redox status; mucosal integrity; growth performance; piglets

Cite this article

LIU Jingbo , CAO Shanchuan , YANG Yong , LIU Ming , ZHANG Hongfu . Effects of Short Chain Fructo-Oligosaccharide on Intestinal Redox Status and Barrier Function of Weaning Piglets[J]. Chinese Journal of Animal Nutrition, 2019 , 31(8) : 3863 -3871 . DOI: 10.3969/j.issn.1006-267x.2019.08.048

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