Maternal Amino Acid Composition in Serum, Amniotic Fluid and Allantoic Fluid Affected by Nutrient Restriction of Goats during Early and Late Gestations

LI Xilin; LI Hengzhi; HE Zhixiong; TAN Zhiliang; YAN Qiongxian

doi:10.3969/j.issn.1006-267x.2019.09.022

2019 , Vol. 31 >Issue 9: 4099 - 4109

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.022

Maternal Amino Acid Composition in Serum, Amniotic Fluid and Allantoic Fluid Affected by Nutrient Restriction of Goats during Early and Late Gestations

LI Xilin ,
LI Hengzhi ,
HE Zhixiong ,
TAN Zhiliang ,
YAN Qiongxian

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1. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Research Center of Livestock & Poultry Sciences, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Hunan Provincial Key Laboratory of Nutritional Physiology and Metabolic Process, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
4. Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China;
5. Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China

Received date: 2019-03-06

Online published: 2019-09-17

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Abstract

This experiment was conducted to investigate the effects of nutrient restriction of goats during early and late gestations on maternal amino acid (AA) composition in serum, amniotic fluid. In early (the 26th to 65th days) and late gestation (the 96th to 135th days), twelve pregnant Xiangdong black goats with similar age[(2.0±0.3) years], parity (2 parities) and body weight were randomly assigned to control group (n=6, ad libitum) and feed intake restricted group (n=6, 40% feed intake restriction). The content of AA in maternal serum, amniotic fluid and allantoic fluid were measured on the 65th and 135th days of pregnancy. The results showed as follows:in early gestation, compared with the control group, the body weight, body length and thoracic circumference of fetuses in feed intake restricted group were significantly increased (P<0.05), the contents of valine (Val), aspartic acid (Asp), proline (Pro), total essential amino acids (EAA) and total non-essential amino acids (NEAA) in serum were significantly decreased (P<0.05), the contents of Val, tyrosine (Tyr) and lysine (Lys) in amniotic fluid were significantly decreased (P<0.05), and the contents of threonine (Thr), methionine (Met), serine (Ser) and glycine (Gly) in allantoic fluid were significantly increased (P<0.05). In late gestation, compared with the control group, the body weight of fetuses in feed intake restricted group was significantly decreased (P<0.05), the contents of Lys, Asp, glutamic acid (Glu) and Pro in serum were significantly decreased (P<0.05), the contents of total EAA, total NEAA and each AA in amniotic fluid were significantly decreased (P<0.05), the content of Glu in allantoic fluid was significantly decreased (P<0.05), and the content of Pro in allantoic fluid was significantly increased (P<0.05). In summary, feed intake restriction during early gestation promotes fetal growth, while inhibits fetal growth during late gestation; feed intake restriction during early and late gestations probably induces some extent variation in AA metabolism of maternal blood and uterus.

Key words： amino acids; feed intake restriction; fetal growth; amniotic fluid; allantoic fluid; goats

Cite this article

LI Xilin , LI Hengzhi , HE Zhixiong , TAN Zhiliang , YAN Qiongxian . Maternal Amino Acid Composition in Serum, Amniotic Fluid and Allantoic Fluid Affected by Nutrient Restriction of Goats during Early and Late Gestations[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4099 -4109 . DOI: 10.3969/j.issn.1006-267x.2019.09.022

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