Effects of Dehulled Soybean Meal Replacing Fish Meal on Growth Performance, Intestinal Digestive Enzyme Activities and Live Immune Indexes of Sillago sihama Forskal

YAN Xiaobo; HE Haolun; TAN Beiping; DU Tao; CHI Shuyan; YANG Qihui; YANG Yuanzhi; DONG Xiaohui

doi:10.3969/j.issn.1006-267x.2019.09.024

2019 , Vol. 31 >Issue 9: 4118 - 4130

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.024

Effects of Dehulled Soybean Meal Replacing Fish Meal on Growth Performance, Intestinal Digestive Enzyme Activities and Live Immune Indexes of Sillago sihama Forskal

YAN Xiaobo ,
HE Haolun ,
TAN Beiping ,
DU Tao ,
CHI Shuyan ,
YANG Qihui ,
YANG Yuanzhi ,
DONG Xiaohui

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1. Guangdong Ocean university College of Fisheries, Zhanjiang 524088, China;
2. Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China

Received date: 2019-03-07

Online published: 2019-09-17

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Abstract

This study was aimed to investigate the dietary dehulled soybean meal (DSM) replacing fish meal on growth performance, liver immune indexes and intestinal digestive enzyme activities of Sillago sihama. A total of 540 fish with an average body weight of (6.0±0.6) g were randomly divided into 6 groups (DSM0, DSM8, DSM16, DSM24, DSM32 and DSM40 groups) with 3 replicates per group and 30 fish per replicate, and fed isonitrogenous and isoenergetic test diets with 0, 8%, 16%, 24%, 32% and 40% DSM replacing fish meal, respectively. Breeding period was 8 weeks. The experimental results showed as follows:1) with the increase of the DSM replacement level, the weight gain rate and specific growth rate increased first and then decreased, and it had the maximum value in DSM24 group. Feed conversion ratio and hepatosomatic index decreased first and then increased, obtained minimum values in DSM24 and DSM16 groups, respectively. There were no significant differences in survival rate and condition factor (P>0.05). 2) Except DSM24 group, DSM replacing fish meal significantly decreased body crude protein and ether extract contents (P<0.05), but had no significant effect on ash content (P>0.05). 3) The activities of superoxide dismutase, alkaline phosphatase and catalase in liver increased first and then decreased with the increased of the levels of DSM replacing fish meal; there was a maximum at the replacement levels of 16% to 24%; the activity of alkaline phosphatase in DSM40 group was significantly lower than that in DSM0 group(P<0.05); the content of malondialdehyde in liver increased gradually, when the replacement level was higher than 32%, the content of malondialdehyde was significantly higher than that in DSM0 group(P<0.05); liver glutamic oxaloacetic transaminase and glutamic-pyruvic transaminase activities showed a steady and rising trend, when the replacement levels were 0 to 32%, there was no significant difference between groups (P>0.05),glutamic oxaloacetic transaminase activity in DSM40 group was significantly higher than that in other groups (P<0.05). 4) DSM did not affect the intestinal lipase activity in the foregut, midgut and hindgut and protease activity in the midgut (P>0.05); and amylase activity in the foregut decreased first and then increased, amylase activity in the midgut, hindgut and protease activity in the foregut and hindgut increased first and then decreased; there was the largest amylase activity in the foregut, the smallest amylase activity in the midgut and hindgut, and the smallest protease activity in the foregut and hindgut, and all were significantly higher than those in DSM0 group (P<0.05). According to the analysis weight gain rate and specific growth rate, the dietary optimal DSM replacement level of fish meal is 29.1% of Sillago sihama.

Key words： DSM; Sillago sihama; growth performance; immune indexes; digestive enzyme

Cite this article

YAN Xiaobo , HE Haolun , TAN Beiping , DU Tao , CHI Shuyan , YANG Qihui , YANG Yuanzhi , DONG Xiaohui . Effects of Dehulled Soybean Meal Replacing Fish Meal on Growth Performance, Intestinal Digestive Enzyme Activities and Live Immune Indexes of Sillago sihama Forskal[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4118 -4130 . DOI: 10.3969/j.issn.1006-267x.2019.09.024

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