Effects of Astaxanthin Supplementation on Growth, Body Color, Antioxidant Capability and Immunity of Kio Carp (Cyprinus carpio L.)

WANG Junhui; XIONG Jianli; ZHANG Dongyang; REN Hongtao; ZHANG Chunnuan

doi:10.3969/j.issn.1006-267x.2019.09.026

2019 , Vol. 31 >Issue 9: 4144 - 4151

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.026

Effects of Astaxanthin Supplementation on Growth, Body Color, Antioxidant Capability and Immunity of Kio Carp (Cyprinus carpio L.)

WANG Junhui ,
XIONG Jianli ,
ZHANG Dongyang ,
REN Hongtao ,
ZHANG Chunnuan

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College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, China

Received date: 2019-02-14

Online published: 2019-09-17

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Abstract

The aim of the present study was to investigate the effects of different supplemental levels of astaxanthin on growth, body color, antioxidant capability and immunity of kio carp (Cyprinus carpio L.). A total of 540 red-white koi carp were randomly distributed into 6 groups with 3 replicates per group and 30 fish per replicate. Fish in the six groups were fed diets containing 0 (control), 200, 400, 600, 800 and 1 000 mg/kg astaxanthin for 8 weeks, respectively. The results showed that the weight gain rate (WGR) and specific growth rate (SGR) increased firstly and then decreased with the supplemental level of astaxanthin increasing, while the feed conversion rate (FCR) showed the opposite trend. Fish fed the diet containing 400 mg/kg astaxanthin showed the lowest FCR and highest WGR and SGR, which were significantly higher than those in control group (P<0.05). The redness (a^*) value of body color in 200 and 400 mg/kg groups was significantly higher than that in control group and 800 mg/kg group (P<0.05). However, there was no significant difference between control group and other supplementation groups (P>0.05). The yellowness (b^*) value of body color and the content of carotenoid in skin were firstly increased and then decreased with the supplemental level of astaxanthin increasing, the 400 mg/kg group showed the highest value and it was significantly higher than that in control group (P<0.05). There was no significant difference in the lightness (L^*) value of body color among groups (P>0.05). Fish fed 400 mg/kg astaxanthin had the highest values of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in liver and lysozyme (LZM), acid phosphatase (ACP), alkaline phosphatase (AKP) activities and complement 3 (C3), complement 4 (C4) contents in serum, which were significantly higher than those in control group and 1 000 mg/kg group (except of ACP activity) (P<0.05). Liver total antioxidant capacity (T-AOC) in 400 and 600 mg/kg groups was significantly higher than that in control group and 800, 1 000 mg/kg groups (P<0.05). However, the liver malondialdehyde (MDA) content showed the lowest value in 400 mg/kg group, and it in 200, 400 and 600 mg/kg groups was significantly lower than that in control group (P<0.05). Those results demonstrate that the diet supplemented with appropriate amount of astaxanthin can increase the growth performance, effectively improve the body color, and enhance the liver antioxidant capability and immunity of kio carp. By comprehensive comparison, the astaxanthin supplemental level of 400 mg/kg is recommended in the diet of kio carp.

Key words： astaxanthin; kio carp (Cyprinus carpio L.); body color; immune; antioxidant

Cite this article

WANG Junhui , XIONG Jianli , ZHANG Dongyang , REN Hongtao , ZHANG Chunnuan . Effects of Astaxanthin Supplementation on Growth, Body Color, Antioxidant Capability and Immunity of Kio Carp (Cyprinus carpio L.)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4144 -4151 . DOI: 10.3969/j.issn.1006-267x.2019.09.026

References

[1] 崔培,姜志强,王雪,等.不同蛋白水平的虾青素饲料对锦鲤体色、生长及免疫的影响[J].上海海洋大学学报,2012,21(3):382-388.
[2] PAGE G,DAVIES S.Tisuue axtaxanthin and canthaxanthin distribution in rainbow trout (Oncorhynchus mykiss) and atlantic salmon (Salmo salar)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2006,143(1):125-132.
[3] 朱艺峰,麦康森.鱼饲料着色剂类胡萝卜素研究进展[J].水生生物学报,2003,27(2):196-200.
[4] 张晓红,吴锐全,王海英,等.虾青素与螺旋藻对血鹦鹉体色的影响[J].大连水产学院学报,2009,24(1):79-82.
[5] BOONYAPAKDEE A,POOTANGON Y,LAUDADIO V,et al.Astaxanthin extraction from golden apple snail (Pomacea canaliculata) eggs to enhance colours in fancy carp (Cyprinus carpio)[J].Journal of Applied Animal Research,2015,43(3):291-294.

[6] WANG W L,ISHIKAWA M,KOSHIO S,et al.Effects of dietary astaxanthin and vitamin E and their interactions on the growth performance,pigmentation,digestive enzyme activity of kuruma shrimp (Marsupenaeus japonicus)[J].Aquaculture Research,2019,50(4):1186-1197.

[7] HAN T,LI X Y,WANG J T,et al.Effects of dietary astaxanthin (AX) supplementation on pigmentation,antioxidant capacity and nutritional value of swimming crab,Portunus trituberculatus[J].Aquaculture,2018,490:169-177.
[8] 裴素蕊,管越强,马云婷.饲料中添加虾青素对凡纳滨对虾生长、存活和抗氧化能力的影响[J].水产科学,2009,28(3):126-129.
[9] YI X W,XU W,ZHOU H H,et al.Effects of dietary astaxanthin and xanthophylls on the growth and skin pigmentation of large yellow croaker Larimichthys croceus[J].Aquaculture,2014,433:377-383.
[10] 张春兰,赵文,徐立蒲,等.微生态制剂和着色剂对锦鲤体色的影响[J].大连海洋大学学报,2011,26(4):328-332.
[11] 陈晓明,徐学明,金征宇.富含虾青素的法夫酵母对金鱼体色的影响[J].中国水产科学,2004,11(1):70-73.
[12] AGATSUMA Y.Aquaculture of the sea urchin (Strongylocentrotus nudus) transplanted from coralline flats in Hokkaido,Japan[J].Journal of Shellfish Research,1998,17(5):1541-1547.
[13] CHRISTIANSEN R,TORRISSEN O J.Growth and survival of Atlantic salmon,Salmo salar L. fed different dietary levels of astaxanthin. Juveniles[J].Aquaculture Nutrition,1996,2(1):55-62.

[14] ZA?KOVÁ I,SERGEJEVOVÁ M,URBAN J,et al.Carotenoid-enriched microalgal biomass as feed supplement for freshwater ornamentals:albinic form of wels catfish (Silurus glanis)[J].Aquaculture Nutrition,2011,17(3):278-286.

[15] 温为庚,林黑着,吴开畅,等.饲料中添加虾青素对斑节对虾生长和免疫指标的影响[J].中山大学学报(自然科学版),2011,50(3):144-146.
[16] 罗刚.鱼类维生素营养研究概况[J].贵州畜牧兽医,2009,33(2):18-19.
[17] YI X,SHEN H,LI J,et al.Effects of dietary vitamin E and astaxanthin on growth,skin colour and antioxidative capacity of large yellow croaker Larimichthys crocea[J].Aquaculture Nutrition,2018,24(1):472-480.

[18] 王锐,费小红.四种增色剂在观赏鱼中应用的研究[J].北京水产,2005(4):37-38.
[19] 崔培,周文礼,刘芳,等.虾青素对红白锦鲤体色、生长的影响[J].水产科技情报,2013,40(1):37-40.
[20] 冷向军,李小勤.水产动物着色的研究进展[J].水产学报,2006,30(1):138-143.
[21] 崔惟东,冷向军,李小勤,等.虾青素和角黄素对虹鳟肌肉着色和肝脏总抗氧化能力的影响[J].水产学报,2009,33(6):987-995.
[22] WANG Y J,CHEN Y H,PAN C H.Effects of dietary supplementation of carotenoids on survival,growth,pigmentation,and antioxidant capacity of characins,Hyphessobrycon callistus[J].Aquaculture,2006,261(2):641-648.

[23] 李建光,胡世然,刘霆,等.四种增色剂对锦鲤的生长、形体、体色和抗氧化能力的影响[J].中国饲料,2009(16):33-36,40.
[24] LEE S H,MIN D B.Eeffets,quenching mechanisms,and kinetics of carotenoids in chlorophyll-sensitized photooxidation of soybean oil[J].Journal of Agricultural and Food Chemistry,1990,38(8):1630-1634.

[25] GRINDE B,LIE Ø,POPPE T,et al.Species and individual variation in lysozyme activity in fish of interest in aquaculture[J].Aquaculture,1988,68(4):299-304.

[26] 崔培,刘芳,杨广,等.虾青素对锦鲤血液及抗氧化指标的影响[J].东北农业大学学报,2013,44(3):89-94.
[27] 曲木,张宝龙,于恩浩,等.虾青素、万寿菊粉对狮子头金鱼生长、血液指标及免疫力的影响[J].生物化工,2018,4(5):45-50.
[28] 麻楠,龙晓文,赵磊,等.饲料中添加合成虾青素对中华绒螯蟹成体雌蟹性腺发育、色泽和抗氧化能力的影响[J].水生生物学报,2017,41(4):755-765.
[29] OKAI Y,HIGASHI-OKAI K.Possible immunomodulating activities of carotenoids in in vitro cell culture experiments[J].International Journal of Immunopharmacology,1996,18(12):753-758.

[30] HILLGARTNER F B,MORIN D,HANSEN R J.Effect of excessive vitamin A intake on muscle protein turnover in the rat[J].The Biochemical Journal,1982,202(2):499-508.

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