This experiment was conducted to study the effects of dietary fat level on nutrient digestibility, serum biochemical indexes and volatile fatty acid composition in rumen fluid of 3-year-old sika deer during velvet antler growth period. Twelve 3-year-old and healthy male sika deer with the average body weight of (115±23) kg were randomly divided into 4 groups with 4 replicates per group and 1 deer per replicate, and they were fed experimental diets containing different fat levels. The diet fat levels were 2% (group Ⅰ), 4% (group Ⅱ) and 6% (group Ⅲ), respectively, and other nutrient levels were basically the same. The experiment was 7 days for adaption and 63 days for trial period. The results showed as follows:1) the digestibility of crude protein in group Ⅰ was significantly higher than that in group Ⅲ (P<0.05), but there was no significant difference among other groups (P>0.05). With the increase of dietary fat level, the digestibility of ether extract, calcium and phosphorus decreased significantly (P<0.01). Dietary fat level had no significant effect on dry matter digestibility (P>0.05). 2) Serum glucose (GLU) content in groups Ⅰ and Ⅲ was significantly higher than that in group Ⅱ (P<0.01), while no significant difference was observed between groups Ⅰ and Ⅲ (P>0.05). Dietary fat level had no significant effects on serum triglyceride (TG), cholesterol (CHO), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) contents (P>0.05). 3) Serum complement 3 (C3) content in group Ⅰ was significantly higher than that in groups Ⅱ and Ⅲ (P<0.05), while no significant difference was observed between groups Ⅱ and Ⅲ (P>0.05). Serum immunoglobulin A (IgA) content in group Ⅰ was significantly higher than that in group Ⅲ (P<0.05), but no significant difference was observed between groups Ⅰ and Ⅱ (P>0.05). Serum immunoglobulin M (IgM), immunoglobulin G (IgG), complement 4 (C4) and insulin (INS) contents were not significantly affected by dietary fat level (P>0.05). 4) Serum total protein (TP) content in group Ⅱ was significantly higher than that in group Ⅲ (P<0.05), while no significant difference was found between groups Ⅰ and Ⅱ (P>0.05). Serum albumin (ALB), globulin (GLB) and urea nitrogen (UN) contents were not significantly affected by dietary fat level (P>0.05). 5) The concentrations of acetic acid, butyric acid and total volatile fatty acids in groups Ⅰ and Ⅱ were significantly higher than those in group Ⅲ (P<0.01), and the difference between groups Ⅰ and Ⅱ was not significant (P>0.05). The concentrations of propionic acid and valproic acid in group Ⅱ were significantly higher than those in group Ⅲ (P<0.05), and the differences between groups Ⅰ and Ⅱ were not significant (P>0.05). There were no significant effects on the concentrations of isobutyric acid and isovaleric acid among groups (P>0.05). From the perspective of saving feeding cost and improving nutrient efficiency, feeding a diet with a fat level of 2% is beneficial for 3-year-old sika deer to meet the healthy production demand during velvet antler growth period.
ZHONG Wei
,
BAO Kun
,
WANG Xiaoxu
,
SUN Weili
,
LIU Hanlu
,
CUI Xuezhe
,
NING Wenchun
,
LI Guangyu
. Effects of Dietary Fat Level on Nutrient Digestibility, Serum Biochemical Indexes and Volatile Fatty Acid Composition in Rumen Fluid of 3-Year-Old Sika Deer during Velvet Antler Growth Period[J]. Chinese Journal of Animal Nutrition, 2019
, 31(9)
: 4162
-4169
.
DOI: 10.3969/j.issn.1006-267x.2019.09.028
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