Phosphorus Absorption and Related Transporter Expression under Low Phosphorus Concentrations in Primarily Cultured Duodenal Epithelial Cells of Broiler Embryos

LYU Na; LYU Lin; LIAO Xiudong; ZAHNG Liyang; LUO Xugang

doi:10.3969/j.issn.1006-267x.2019.09.031

2019 , Vol. 31 >Issue 9: 4186 - 4193

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.031

Phosphorus Absorption and Related Transporter Expression under Low Phosphorus Concentrations in Primarily Cultured Duodenal Epithelial Cells of Broiler Embryos

LYU Na ,
LYU Lin ,
LIAO Xiudong ,
ZAHNG Liyang ,
LUO Xugang

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Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2019-03-06

Online published: 2019-09-17

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Abstract

The aim of the study was to investigate the phosphorus absorption and related transporter expression under low phosphorus concentrations in primarily cultured duodenal epithelial cells of broiler embryos. A single-factor completely randomized design was used in this experiment, the cells were randomly assigned to 5 groups with 6 replicates per group, and then treated with 0 (control group), 0.5, 1.0, 2.0 and 4.0 mmol/L phosphorus for 87 min, respectively. The results showed that different phosphorus concentrations had significant effect on phosphorus absorption percentage in duodenal epithelial cells (P<0.05), and the phosphorus absorption percentage was linear or quadratic increased with phosphorus concentration increased (P<0.05). Compared with the control group, the mRNA relative expressions of type Ⅱb sodium-phosphate cotransporter (NaPi-Ⅱb) and inorganic phosphate transporter 1 (PiT-1) in duodenal epithelial cells of 4.0 mmol/L group were significantly decreased (P<0.05), but the mRNA relative expression of inorganic phosphate transporter 2 (PiT-2) in duodenal epithelial cells of 4.0 mmol/L group had no significant change (P>0.05). Different phosphorus concentrations had no significant effects on the protein expressions of NaPi-Ⅱb, PiT-1 and PiT-2 in duodenal epithelial cells (P>0.05). In conclusion, with the incubation solution phosphorus concentration increased, the cell phosphorus absorption percentage increase; when the incubation solution phosphorus concentration is 4.0 mmol/L, the primarily cultured duodenal epithelial cells of broiler embryos can regulate the phosphorus absorption by reducing the mRNA expressions of NaPi-Ⅱb and PiT-1.

Key words： duodenal epithelial cells; broiler embryos; phosphorus; absorption

Cite this article

LYU Na , LYU Lin , LIAO Xiudong , ZAHNG Liyang , LUO Xugang . Phosphorus Absorption and Related Transporter Expression under Low Phosphorus Concentrations in Primarily Cultured Duodenal Epithelial Cells of Broiler Embryos[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4186 -4193 . DOI: 10.3969/j.issn.1006-267x.2019.09.031

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