In vitro Study on Effects of Different Proportions of Hops Pellets and Cerium Chloride and Its Combination on Rumen Fermentation

CHEN Panliang; LI Yan; CAO Yufeng; LI Qiufeng; GAO Yanxia; LI Jianguo

doi:10.3969/j.issn.1006-267x.2019.09.033

2019 , Vol. 31 >Issue 9: 4200 - 4207

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.033

In vitro Study on Effects of Different Proportions of Hops Pellets and Cerium Chloride and Its Combination on Rumen Fermentation

CHEN Panliang ,
LI Yan ,
CAO Yufeng ,
LI Qiufeng ,
GAO Yanxia ,
LI Jianguo

Expand

1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, China;
2. College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China;
3. Embryo Engineering and Technological Center of Cattle and Sheep of Hebei, Baoding 071001, China

Received date: 2019-03-07

Online published: 2019-09-17

Fold

Abstract

The purpose of this experiment was to investigate the effects of different proportions of hops pellets and cerium chloride and its combination on rumen fermentation through a in vitro study. The total mixed ration (TMR) and rumen fluid of high-yield dairy cows were used as fermentation substrate and fermentation microflora. A 4×4 two-factor experiment design was adopted, the hops pellets were set 4 concentrations of blank (0 mg/L), low (L, 3.34 mg/L), middle (M, 6.67 mg/L) and high (H, 10.00 mg/L), respectively; the cerium chloride were set 4 concentrations of blank (0 mg/L), low (l, 1.33 mg/L), middle (m, 1.60 mg/L) and high (h, 1.87 mg/L), respectively, to meet the hops pellets contents of dry matter fermentation substrate were 0, 0.5, 1.0 and 1.5 g/kg, respectively; and the cerium chloride contents were 0, 0.20, 0.24 and 0.28 g/kg, respectively. According to different combinations of hops pellets and cerium chloride, 16 groups (5 replicates per group) were divided into C (blank control), l, m, h, L, Ll, Lm, Lh, M, Ml, Mm, Mh,H, Hl, Hm and Hh. Anaerobic fermentation was carried out for 24 h using the American ANKOM RFS gas measurement system, and the pH, ammonia nitrogen (NH₃-N) concentration, microbial crude protein (MCP) yield, dry matter digestibility (DMD), gas production (GP) and the concentrations of acetic acid, propionic acid and total volatile fat acids (TVFA) were detected when fermentation stopped. The results showed as follows:1) among the groups adding hops pellets alone, the NH₃-N concentration in groups L and M were significantly decreased than that in group C (P<0.05), the MCP yield in group M was significantly increased than that in group C (P<0.05). 2) Among the groups adding cerium chloride alone, the MCP yield in groups l, m and h was significantly increased than that in group C (P<0.05 or P<0.01), the DMD, GP and concentrations of acetic acid, propionic acid and TVFA in group l were significantly increased than those in group C (P<0.05). 3) The combination of hops pellets and cerium chloride had significant interactive effects on the NH₃-N, acetic acid, propionic acid and TVFA concentrations and MCP yield (P<0.05). The NH₃-N concentration in all groups except groups Hh and Lh was significantly decreased that in group C (P<0.01), and that in groups Hl and Ll was lower; the MCP yield in all groups except group Lh was significantly increased that in group C (P<0.05 or (P<0.01), and that in groups Lm, Ll, Ml was higher; and the TVFA concentration in groups Mh, Lh and Ll was significantly increased that in group C (P<0.05). According to the comprehensive analysis of each index, groups M, l and Ll are the optimal group among hops pellets and cerium chloride alone and its combination groups, the rumen fermentation performance effect can be improved in the order of group Ll > group l > group M.

Key words： hops pellets; cerium chloride; in vitro study; rumen fermentation

Cite this article

CHEN Panliang , LI Yan , CAO Yufeng , LI Qiufeng , GAO Yanxia , LI Jianguo . In vitro Study on Effects of Different Proportions of Hops Pellets and Cerium Chloride and Its Combination on Rumen Fermentation[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4200 -4207 . DOI: 10.3969/j.issn.1006-267x.2019.09.033

References

[1] KOHN R A,DINNEEN M M,RUSSEK-COHEN E.Using blood urea nitrogen to predict nitrogen excretion and efficiency of nitrogen utilization in cattle,sheep,goats,horses,pigs,and rats[J].Journal of Animal Science,2005,83(4):879-889.

[2] HUHTANEN P,HRISTOV A N.A meta-analysis of the effects of dietary protein concentration and degradability on milk protein yield and milk N efficiency in dairy cows[J].Journal of Dairy Science,2009,92(7):3222-3232.

[3] OSHUGI M,BASNET P,KADOTA S,et al.Antibacterial activity of traditional medicines and an active constituent lupulone from Humulus lupulus against Helicobacter pylori[J].Journal of Traditional Chinese Medicine,1997,14(3):186-191.
[4] MOIR M.Hops-a millennium review[J].Journal of the American Society of Brewing Chemists,2000,58(4):131-146.

[5] LAVREN ?I?A,LEVART A,KOŠIR I J,et al.Influence of two hop (Humulus lupulus L.) varieties on in vitro dry matter and crude protein degradability and digestibility in ruminants[J].Journal of the Science of Food and Agriculture,2014,94(6):1248-1252.

[6] FLYTHE M D,AIKEN G E,GELLIN G L,et al.Hops (Humulus lupulus) β-acid as an inhibitor of caprine rumen hyper-ammonia-producing bacteria in vitro[J].Agriculture,Food and Analytical Bacteriology,2015,5(1):29-36.
[7] FLYTHE M D.The antimicrobial effects of hops (Humulus lupulus L.) on ruminal hyper ammonia-producing bacteria[J].Letters in Applied Microbiology,2009,48(6):712-717.
[8] 刘江波,薛白,闫天海,等.不同氯化铈水平对南江黄羊瘤胃体外发酵的影响[J].中国畜牧杂志,2012,48(5):31-34.
[9] LIN S X,WEI C,ZHAO G Y,et al.Effects of supplementing rare earth element cerium on rumen fermentation,nutrient digestibility,nitrogen balance and plasma biochemical parameters in beef cattle[J].Journal of Animal Physiology and Animal Nutrition,2015,99(6):1047-1055.

[10] FLYTHE M D,KAGAN I A,WANG Y X,et al.Hops (Humulus lupulus L.) bitter acids:modulation of rumen fermentation and potential as an alternative growth promoter[J].Frontiers in Veterinary Science,2017,4:131.
[11] 张婷婷.稀土元素镧、铈、镨对体外瘤胃发酵、甲烷生成及微生物区系的影响[D].博士学位论文.北京:中国农业大学,2015.
[12] 吴丹丹,吕永艳,孙友德,等.小肽和稀土不同添加量组合对奶牛产奶性能及氮排泄的影响[J].中国畜牧杂志,2016,52(17):62-67.
[13] 梁欢,赵向辉,许兰娇,等.烟酸、果寡糖及柠檬酸稀土组合对肉牛瘤胃体外发酵特性及菌群结构的影响[J].动物营养学报,2018,30(5):1996-2005.
[14] 于静.添喂稀土对绵羊消化代谢的影响[D].硕士学位论文.乌鲁木齐:新疆农业大学,2009.
[15] GOERING H K,VAN SOEST P J.Forage fiber analysis:apparatus,reagents,procedures,and some applica-tions[M].Washington,D.C.:Agricultural Research Service,United States Department of Agriculture,1970:379.
[16] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,31(6/7):37.
[17] COTTA M A,RUSSELL J B.Effect of peptides and amino acids on efficiency of rumen bacterial protein synthesis in continuous culture[J].Journal of Dairy Science,1982,65(2):226-234.

[18] 中华人民共和国国家质量监督检验检疫总局.饲料中粗蛋白的测定[M].北京:中国标准出版社,2002.
[19] KHORASANI G R,OKINE E K,KENNELLY J J.Forage source alters nutrient supply to the intestine without influencing milk yield[J].Journal of Dairy Science,1996,79(5):862-872.

[20] 张文璐,李杰,吕元勋.体外产气法与尼龙袋法评定粗饲料干物质降解率的相关性分析[J].饲料工业,2009,30(7):30-32.
[21] 刘江波.稀土元素对南江黄羊瘤胃发酵、生长性能及血液指标的影响[D].硕士学位论文.雅安:四川农业大学,2012.
[22] OZUTSUMI Y,TAJIMA K,TAKENAKA A,et al.Real-time PCR detection of the effects of protozoa on rumen bacteria in cattle[J].Current Microbiology,2006,52(2):158-162.

[23] YANG W Z,LAARMAN A,HE M L,et al.Effect of rare earth elements on in vitro rumen microbial fermentation and feed digestion[J].Animal Feed Science and Technology,2009,148(2/3/4):227-240.
[24] 曹永范,张冬艳,王丽,等.稀土离子对纤维素酶活性的影响[J].内蒙古工业大学学报,2010,29(2):104-106.
[25] 余兴莲.稀土离子对纤维酶活性的影响及其机理[D].硕士学位论文.宁波:宁波大学,2008.
[26] NARVAEZ N,WANG Y X,XU Z,et al.Effects of hop varieties on ruminal fermentation and bacterial community in an artificial rumen (rusitec)[J].Journal of the Science of Food and Agriculture,2013,93(1):45-52.

[27] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.
[28] 张吉鹍,邹庆华,王金芬,等.稻草与多水平苜蓿混合瘤胃体外发酵组合效应的整体研究[J].饲料工业,2011,32(17):40-48.
[29] 王之盛,李胜利.反刍动物营养学[M].北京:中国农业出版社,2016.
[30] CALSAMIGLIA S,FERRET A,REYNOLDS C K,et al.Strategies for optimizing nitrogen use by ruminants[J].Animal,2010,4(7):1184-1196.

[31] BACH A,CALSAMIGLIA S,STERN M D.Nitrogen metabolism in the rumen[J].Journal of Dairy Science,2005,88(Suppl.1):E9-E21.
[32] 申军士.日粮能氮释放同步性对奶牛瘤胃代谢、生产效率与性能的影响研究[D].博士学位论文.杭州:浙江大学,2013.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References