Effects of Chitosan on Arachidonic Acid Immune Regulating Pathway in Mid-Lactating Dairy Cows

LI Tiyu; YAN Sumei; SHI Binlin; ZHANG Pengfei

doi:10.3969/j.issn.1006-267x.2019.09.035

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 9: 4218 - 4225

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.035

Effects of Chitosan on Arachidonic Acid Immune Regulating Pathway in Mid-Lactating Dairy Cows

LI Tiyu ,
YAN Sumei ,
SHI Binlin ,
ZHANG Pengfei

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2019-02-21

Online published: 2019-09-17

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Abstract

This experiment was conducted to study the effects of chitosan on arachidonic acid immune regulating pathway in mid-lactating dairy cows. A total of forty healthy mid-lactating Holstein cows with similar milk yield, days in lactation and parity were randomly divided into five groups with eight cows per group. The five experimental diets contained 0 (control group), 500, 1 000, 1 500 and 2 000 mg/kg chitosan, respectively. Feeding trial lasted for 60 days, and was divided two phases:prophase (day 1 to 30) and anaphase (day 31 to 60). Cows had free access to feed and water during the feeding trial. The results showed as follows:with the of chitosan supplemental level increasing, the concentrations of arachidonic acid (AA) and leukotriene B₄ (LTB₄), the activity of 5-lipoxygenase (5-LOX) in serum and the gene expression levels of cytosolic phospholipase A₂ (cPLA₂) and 5-LOX in peripheral blood lymphocytes (PBLs) showed significant linear increasing effects on anaphase (day 60) (P<0.05). The concentrations of prostaglandin E₂ (PGE₂) and LTB₄, the activities of cPLA₂ and cyclooxygenase-2 (COX-2) in serum and the gene expression levels of COX-2 and 5-LOX in PBLs exhibited significant quadratic increasing effects on anaphase (day 60) (P<0.05). The gene expression level of cPLA₂ in PBLs showed extremely significant linear and quadratic increasing effects on prophase (day 30) (P<0.01). The gene expression level of 5-LOX in PBLs exhibited significant quadratic increasing effects on prophase (day 30) (P<0.05). When the chitosan supplemental level was 1 500 mg/kg, all above indices got the best performance. The results indicate that chitosan can affect the immune function of mid-lactating dairy cows by increasing the gene expression levels of cPLA₂, COX-2, and 5-LOX, improving the activities of cPLA₂, COX-2 and 5-LOX, stimulating the releasing of main factors in AA metabolic pathway such as AA, PGE₂ and LTB₄ from immunological cells, and the dosage of 1 500 mg/kg chitosan has the best immunoregulatory effect.

Key words： chitosan; dairy cows; arachidonic acid; immune regulating

Cite this article

LI Tiyu , YAN Sumei , SHI Binlin , ZHANG Pengfei . Effects of Chitosan on Arachidonic Acid Immune Regulating Pathway in Mid-Lactating Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2019 , 31(9) : 4218 -4225 . DOI: 10.3969/j.issn.1006-267x.2019.09.035

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